Identifying critical states of hepatocellular carcinoma based on landscape dynamic network biomarkers

Hepatocellular carcinoma (HCC) is the major histological form of primary liver cancer. It has usually reached the disease state once the patient is diagnosed since there are no specific symptoms in the early stages of HCC. This fact increases the difficulty of curing HCC. Recently, quantities of evidence have shown that many mathematical methods (such as dynamic network biomarkers, DNB) can be used to detect critical states or tipping points of complex diseases. However, it is difficult to apply the DNB theory to the clinic since multiple samples are generally unavailable for individual patient. This paper constructs a novel method based on landscape dynamic network biomarkers (L-DNB), which aims to detect early warning signals from cirrhosis state to very advanced HCC state in individual patient. The selected dataset contains multiple samples for each HCC state. A score that indicates the disease characteristics is calculated for each sample by RNA-seq data, and several scores constitute a distribution in the same state. Quantifying the statistical characteristics of these distributions and determining that low-grade dysplastic and high-grade dysplastic are the critical states of HCC. These results can provide scientific advice for early warning indicators and optimal treatment time for HCC.     

Clinicopathological value and underlying molecular mechanism of annexin A2 in 992 cases of thyroid carcinoma

BackgroundThyroid carcinoma (THCA) is one of the most frequent endocrine cancers and has increasing morbidity. Annexin A2 (ANXA2) has been found to be highly expressed in various cancers; however, its expression level and potential mechanism in THCA remain unknown. This study investigated the clinicopathological value and primary molecular machinery of ANXA2 in THCA.Material and MethodsPublic RNA-sequencing and microarray data were obtained and analyzed with ANXA2 expression in THCA and corresponding non-cancerous thyroid tissue. A Pearson correlation coefficient calculation was used for the acquisition of ANXA2 coexpressed genes, while edgR, limma, and Robust Rank Aggregation were employed for differentially expressed gene (DEG) in THCA. The probable mechanism of ANXA2 in THCA was predicted by gene ontology and pathway enrichment. A dual-luciferase reporter assay was employed to confirm the targeting relationships between ANXA2 and its predicted microRNA (miRNA).ResultsExpression of ANXA2 was significantly upregulated in THCA tissues with a summarized standardized mean difference of 1.09 (P < 0.0001) based on 992 THCA cases and 589 cases of normal thyroid tissue. Expression of ANXA2 was related to pathologic stage. Subsequently, 1442 genes were obtained when overlapping 4542 ANXA2 coexpressed genes with 2248 DEGs in THCA; these genes were mostly enriched in pathways of extracellular matrix-receptor interaction, cell adhesion molecules, and complement and coagulation cascades. MiR-23b-3p was confirmed to target ANXA2 by dual-luciferase reporter assay.ConclusionsUpregulated expression of ANXA2 may promote the malignant biological behavior of THCA by affecting the involving pathways or being targeted by miR-23b-3p.     

Binding evaluation of isoform 1 fromCratylia mollis lectin to human mammary tissues

The phenomenon of altered carbohydrates in transformed cell surfaces has been studied through histochemical techniques using lectins. Specific binding patterns to normal and transformed mammary tissues were evaluated by Isoform 1 fromCratylia mollis lectin (Cra Iso 1). Protocols using a direct method, incubation of Cra Iso I conjugated to peroxidase (Cra Iso 1-Per) with mammary tissues, followed by diaminobenzidine and hydrogen peroxidase interaction, were performed. Neoplastic tissues, marked by Cra Iso 1, showed a higher intensity of staining than normal ones, in comparison withCanavalia ensiformis lectin, Concanavalin A (Con A), conjugated to peroxidase (Con A-Per). The assay with Cra Iso 1 also indicated a possible utilization of this lectin to characterize normal and transformed mammary cells.     

Numerical algorithms for a free boundary problem model of DCIS and a related inverse problem

ABSTRACT

The Ductal carcinoma in situ (DCIS) model has wide applications in the diagnosis of breast cancer, and has been attracted much attention in recent years. In this paper, an effective method has been provided for solving the direct problem of the DCIS model. Moreover, the uniqueness result is established for a kind of inverse problems of DCIS model. Based on the uniqueness theorem, an optimization method has been developed for dealing with the related inverse problem of the DCIS model. The numerical experiments show that the algorithms in this paper are efficient, accurate, robust against noise and fast.     

Molecular design and synthesis of new heterobivalent compounds based on chlorambucil and colchicine

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硒对不同水体致癌因子诱发健康人微核的拮抗作用

采用微核检测技术，研究了亚硒酸钠对启东肝癌高发区不同类型饮用水诱发健康人外周血淋巴细胞微核的拮抗作用。结果表明：（１）各实验水体对细胞的毒性作用，宅沟水〉泯沟水〉自来水〉双蒸水。微核率随着实验水体剂量的增加而升高；（２）１ｍｇ／Ｌ的亚硒酸钠浓度对泯沟水，自来水诱发的微核效应有是显的抑制和／或拮抗作用，呈现明显的剂量－效应关系，提示肝癌高发区饮用水体中确存在与肝癌发病相关的致畸因子，补硒确有防与治原     

Investigation on the viscoelasticity of synchronous hepatocellular carcinoma cells

Having made use of the micropipette aspiration technique, we here investigated the viscoelastic properties of hepatocellular carcinoma (HTC) cells from the view of cell cycle. The synchronous G₁ and S phase cells were achieved through thymine-2-desoryriboside and colchicine sequential blockage method and double thymine-2-desoryriboside blockage method, respectively. The synchronization results detected with flow cytometer showed that it could meet the requirements of the experiments nicely. Experimental results were analyzed with a standard linear solid viscoelastic model, in which an elastic element, K₁, is in parallel with a Maxwell element composed of another elastic element, K₂, in series with a viscous element, μ. The results indicated that high K₁, K₂ values and low μ value was the general characteristics of the cells; G₁ phase cells had higher K₁ values and low μ value than S phase cells, which endowed G₁ phase cells with higher elasticity and faster passive deformability than S phase cells. The results maybe also reflected the difference of cytoskeleton between G₁ and S phase cells, and suggested that G₁ phase cells were more suitable for surviving and metastasis in blood circulation than S phase cells.     

胃癌与七种元素关系的分析

57例胃癌癌灶内锌值为(13.740±4.150)×10~(-6),低于癌边缘组织(15.677±4.567)×10~(-6)(P<0.01),亦低于正常组织(15.897±3.580)×10~(-6)(P<0.01)。癌灶铜值为(7.91±6.19)×10~(-6),高于正常组织(5.62±12.01)×10~(-6)(P<0.05),癌灶铜/锌比值亦高于正常组织(P<0.01),但与边缘组织无显著性差异。分化较好的胃腺癌与未分化癌间七种微量元素含量均无显著性差异。这些结果提示,胃癌组织中锌值的降低与铜/锌比值的升高可作为胃癌的辅助诊断指标,而且锌值的检测更具意义。但与分化程度无关。     

Inhibitory Effect of Anti-HER-2 Anti-CD3 Bi-specific Antibody on the Growth of Gastric Carcinoma

Introduction Gastriccancerisoneofthemostcommonlyen counteredmalignantdiseasesworldwide,especiallyin AsiaandAfrica[1].Thecombinationofoperation,chemotherapyandradiotherapyisusedfortreatinggas triccarcinoma.However,the5yearsurvivalrateof patientsofgastricca…     

鼻咽癌高低发区中钙与鼻咽癌发病关系再调查的结果

报告了１９９４～１９９５年间在鼻咽癌高发区（四会）与低发区（梅州）进行钙与鼻咽癌发病关系的再次调查结果．高发区的井水钙低，正常人的血清钙亦低，差异有显著性意义；鼻咽癌病人的血清钙又低于正常人，Ｐ＜０．０５；上述结论与９３年的研究结果一致。四会境内肝及消化道恶性肿瘤死亡率不存在地区性差异，提示钙与鼻咽癌发病有密切关系。