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161.
162.
Andrew J. Thompson Dr. Jerome Dabin Javier Iglesias‐Fernández Dr. Albert Ardèvol Dr. Zoran Dinev Assoc. Prof. Spencer J. Williams Dr. Omprakash Bande Dr. Aloysius Siriwardena Carl Moreland Dr. Ting‐Chou Hu David K. Smith Prof. Harry J. Gilbert Prof. Carme Rovira Prof. Gideon J. Davies 《Angewandte Chemie (International ed. in English)》2012,51(44):11171-11171
163.
《Biomedical chromatography : BMC》2018,32(5)
In the present study, one‐step purification of angiotensin‐converting enzyme (ACE, peptidyldipeptidase A, EC 3.4.15.1), responsible for regulation of blood pressure, was achieved using affinity chromatography from human plasma. The enzyme was purified 12,860‐fold with a specific activtiy of 5080 EU/mg protein. Optimum temperature and pH were determined for the enzyme as 35–40°C and pH 7.4–7.5, respectively. The purity of ACE was determined by SDS–PAGE and the enzyme showed two bands at 60 and 70 kDa on the gel. The native molecular weight of ACE was found to be 260 kDa by gel filtration chromatography, demonstrating that the enzyme has a heterodimeric structure. Natural fatty acids of Nigella sativa (Ranunculaceae) were isolated by means of column chromatography. The structures of these compounds were determined using NMR and GC‐MS. The results showed that high concentrations of linoleic, oleic and palmitic acids were isolated from the plant. The effect of six fractions (Fr 1–6) on ACE activity was examined. Fraction 3 increased the ACE activity while the other fractions decreased the enzyme activity. The concentrations of the fractions inhibiting the half‐maximum activity of the enzyme were calculated as 1.597 mg/mL for Fr 1, 0.053 mg/mL for Fr 2, 0.527 mg/mL for Fr 4, 0.044 mg/mL for Fr 5 and 0.136 mg/mL for Fr 6 using a Lineweaver–Burk graph. 相似文献
164.
Dr. Oleksii Zozulia Dr. Liam R. Marshall Dr. Inhye Kim Eric M. Kohn Prof. Dr. Ivan V. Korendovych 《Chemistry (Weinheim an der Bergstrasse, Germany)》2021,27(17):5388-5392
The self-assembly of short peptides gives rise to versatile nanomaterials capable of promoting efficient catalysis. We have shown that short, seven-residue peptides bind hemin to produce functional catalytic materials which display highly efficient peroxidation activity, reaching a catalytic efficiency of 3×105 m −1 s−1. Self-assembly is essential for catalysis as non-assembling controls show no activity. We have also observed peroxidase activity even in the absence of hemin, suggesting the potential to alter redox properties of substrates upon association with the assemblies. These results demonstrate the practical utility of self-assembled peptides in various catalytic applications and further support the evolutionary link between amyloids and modern-day enzymes. 相似文献
165.
Determination of the inhibitory effect of green tea extract on glucose‐6‐phosphate dehydrogenase based on multilayer capillary enzyme microreactor 下载免费PDF全文
Mohamed Amara Camara Miaomiao Tian Xiaoxia Liu Xin Liu Yujia Wang Jiqing Yang Li Yang 《Biomedical chromatography : BMC》2016,30(8):1210-1215
Natural herbal medicines are an important source of enzyme inhibitors for the discovery of new drugs. A number of natural extracts such as green tea have been used in prevention and treatment of diseases due to their low‐cost, low toxicity and good performance. The present study reports an online assay of the activity and inhibition of the green tea extract of the Glucose 6‐phosphate dehydrogenase (G6PDH) enzyme using multilayer capillary electrophoresis based immobilized enzyme microreactors (CE‐IMERs). The multilayer CE‐IMERs were produced with layer‐by‐layer electrostatic assembly, which can easily enhance the enzyme loading capacity of the microreactor. The activity of the G6PDH enzyme was determined and the enzyme inhibition by the inhibitors from green tea extract was investigated using online assay of the multilayer CE‐IMERs. The Michaelis constant (Km) of the enzyme, the IC50 and Ki values of the inhibitors were achieved and found to agree with those obtained using offline assays. The results show a competitive inhibition of green tea extract on the G6PDH enzyme. The present study provides an efficient and easy‐to‐operate approach for determining G6PDH enzyme reaction and the inhibition of green tea extract, which may be beneficial in research and the development of natural herbal medicines. Copyright © 2016 John Wiley & Sons, Ltd. 相似文献
166.
Enzymatic Synthesis of Acylphloroglucinol 3‐C‐Glucosides from 2‐O‐Glucosides using a C‐Glycosyltransferase from Mangifera indica 下载免费PDF全文
Dawei Chen Lili Sun Dr. Ridao Chen Dr. Kebo Xie Prof. Dr. Lin Yang Prof. Dr. Jungui Dai 《Chemistry (Weinheim an der Bergstrasse, Germany)》2016,22(17):5873-5877
A green and cost‐effective process for the convenient synthesis of acylphloroglucinol 3‐C‐glucosides from 2‐O‐glucosides was exploited using a novel C‐glycosyltransferase (MiCGTb) from Mangifera indica. Compared with previously characterized CGTs, MiCGTb exhibited unique de‐O‐glucosylation promiscuity and high regioselectivity toward structurally diverse 2‐O‐glucosides of acylphloroglucinol and achieved high yields of C‐glucosides even with a catalytic amount of uridine 5′‐diphosphate (UDP). These findings demonstrate for the first time the significant potential of a single‐enzyme approach to the synthesis of bioactive C‐glucosides from both natural and unnatural acylphloroglucinol 2‐O‐glucosides. 相似文献
167.
Dr. Lan Hu Prof. Dr. Yan Zhao 《Chemistry (Weinheim an der Bergstrasse, Germany)》2019,25(32):7702-7710
Outcomes of chemical reactions are generally dominated by the intrinsic reactivities of reaction partners, but enzymes frequently override such constraints to transform less reactive molecules in the presence of more reactive ones. Despite the attractiveness of such catalysis, it is difficult to build synthetic catalysts with these features. Micellar imprinting is a powerful method to create template-complementary binding sites inside protein-sized water-soluble nanoparticles. When a photocleavable functional monomer was used to bind two phosphonate/phosphate templates as transition-state analogues, active sites with predetermined size and shape were formed inside doubly cross-linked micelles through molecular imprinting. Postmodification replaced the binding group with a catalytic pyridyl group, forming highly selective artificial esterases. The catalysts displayed enzyme-like kinetics and turnover numbers that were in the hundreds. The selectivity of the catalysts, derived from the substrate-complementary imprinted active sites, enabled transformation of less reactive esters in the presence of more reactive ones. 相似文献
168.
Two-Photon-Induced CO-Releasing Molecules as Molecular Logic Systems in Solution,Polymers, and Cells
Dr. Vadde Ramu Dr. Gandra Upendar Reddy Dr. Jingjing Liu Patrick Hoffmann Dr. Rudrakant Sollapur Dr. Ralf Wyrwa Dr. Stephan Kupfer Prof. Dr. Christian Spielmann Dr. Sylvestre Bonnet Prof. Dr. Ute Neugebauer Dr. Alexander Schiller 《Chemistry (Weinheim an der Bergstrasse, Germany)》2019,25(36):8453-8458
Phototherapeutic applications of carbon monoxide (CO)-releasing molecules are limited because they require harmful UV and blue light for activation. We describe two-photon excitation with NIR light (800 nm)-induced CO-release from two MnI tricarbonyl complexes bearing 1,8-naphthalimide units ( 1 , 2 ). Complex 2 behaves as a logic OR gate in solution, nonwovens, and in HeLa cells. CO release, indicated by fluorescence enhancement, was detected in solution, nonwoven, and HeLa cells by single- (405 nm) and two-photon (800 nm) excitation. The photophysical properties of 1 and 2 have been measured and supported by DFT and TDDFT quantum chemical calculations. Both photoCORMs are stable in the dark in solution and noncytotoxic, leading to promising applications as phototherapeutics with NIR light. 相似文献
169.
Dr. Benjamin R. Lichman Prof. Dr. Sarah E. O'Connor Dr. Hajo Kries 《Chemistry (Weinheim an der Bergstrasse, Germany)》2019,25(28):6864-6877
Long sought after [4+2] cyclases have sprouted up in numerous biosynthetic pathways in recent years, raising hopes for biocatalytic solutions to cycloaddition catalysis, an important problem in chemical synthesis. In a few cases, detailed pictures of the inner workings of these catalysts have emerged, but intense efforts to gain deeper understanding are underway by means of crystallography and computational modelling. This Minireview aims to shed light on the catalytic strategies that this highly diverse family of enzymes employs to accelerate and direct the course of [4+2] cycloadditions with reference to small-molecule catalysts and designer enzymes. These catalytic strategies include oxidative or reductive triggers and lid-like movements of enzyme domains. A precise understanding of natural cycloaddition catalysts will be instrumental for customizing them for various synthetic applications. 相似文献
170.
High-Molecular-Weight Polynucleotides by Transferase-Catalyzed Living Chain-Growth Polycondensation 下载免费PDF全文
Dr. Lei Tang Luis A. Navarro Jr. Prof. Ashutosh Chilkoti Prof. Stefan Zauscher 《Angewandte Chemie (International ed. in English)》2017,56(24):6778-6782
We present terminal deoxynucleotidyl transferase-catalyzed enzymatic polymerization (TcEP) for the template-free synthesis of high-molecular-weight, single-stranded DNA (ssDNA) and demonstrate that it proceeds by a living chain-growth polycondensation mechanism. We show that the molecular weight of the reaction products is nearly monodisperse, and can be manipulated by the feed ratio of nucleotide (monomer) to oligonucleotide (initiator), as typically observed for living polymerization reactions. Understanding the synthesis mechanism and the reaction kinetics enables the rational, template-free synthesis of ssDNA that can be used for a range of biomedical and nanotechnology applications. 相似文献