Novel bivalent positive allosteric AMPA receptor modulator of bis-amide series

1. Download : Download high-res image (157KB)
2. Download : Download full-size image

     

A New Model of Coupled Hindmarsh-Rose Neurons

A new model of two coupled neurons is presented by the partly di?usive HindmarshRose equations. The solution semi?ow exhibits globally absorbing characteristics. As the main result, the self-synchronization of the coupled neurons at a uniform rate is proved, which can be extended to complex neuronal networks.     

An AIE‐Based Probe for Rapid and Ultrasensitive Imaging of Plasma Membranes in Biosystems

The abnormality of the plasma membrane (PM) is an important biomarker for cell status and many diseases. Hence, visualizing the PM, especially in complex systems, is an emerging field in the life sciences, especially in low‐resource settings. Herein, we developed a water‐soluble PM‐specific probe utilizing electrostatic and hydrophobic interaction strategies with aggregation‐induced emission as the signal output. The probe could image the PM with many advanced features (wash‐free, ultrafast staining process, excellent PM specificity, and good biocompatibility), which were demonstrated by the PM imaging of neurons. The probe allowed for the first time the imaging of erythrocytes in the complex brain environment through a fluorescence‐based method. Moreover, the PM of the epidermal and partial view of the eyeball structure of live zebrafish are also revealed.     

Screening of Hydrogels for Human Pluripotent Stem Cell–Derived Neural Cells: Hyaluronan‐Polyvinyl Alcohol‐Collagen‐Based Interpenetrating Polymer Network Provides an Improved Hydrogel Scaffold

There is a clear need for novel in vitro models, especially for neuronal applications. Development of in vitro models is a multiparameter task consisting of cell‐, biomaterial‐, and environment‐related parameters. Here, three different human origin neuronal cell sources are studied and cultured in various hydrogel 3D scaffolds. For the efficient evaluation of complex results, an indexing method for data is developed and used in principal component analysis (PCA). It is found that no single hydrogel is superior to other hydrogels, and collagen I (Col1) and hyaluronan–poly(vinyl alcohol) (HA1‐PVA) gels are combined into an interpenetrating network (IPN) hydrogel. The IPN gel combines cell supportiveness of the collagen gel and stability of the HA1‐PVA gel. Moreover, cell adhesion is studied in particular and it is found that adhesion of neurons differs from that observed for fibroblasts. In conclusion, the HA1‐PVA‐col1 hydrogel is a suitable scaffold for neuronal cells and supports adhesion formation in 3D.     

Bursting synchronization in non-locally coupled maps

Neuron activity presents two timescales, a fast one related to action-potential spiking, and a slow timescale in which bursting takes place. Bursting activity in neuron ensembles can be synchronized, meaning the adjustment of the bursting phases due to coupling. We investigated bursting synchronization in a non-locally coupled lattice using a two-dimensional map to describe neuron activity. The coupling involves all sites in a lattice, the corresponding strength decreasing with the lattice distance in a power-law fashion. We observed bursting synchronization for wide intervals of the coupling parameters. We also investigated the bursting synchronization of the ensemble with an external time-periodic signal applied to one or more selected neurons.     

弱激光对神经元钾离子通道特性影响的实验研究

利用波长为670 nm、功率为5 mW的半导体激光器照射急性分离的大鼠海马CA3区锥体神经元，应用全细胞膜片钳技术研究其瞬时外向钾离子通道特性.实验发现：弱激光对瞬时外向钾电流具有抑制作用,并且该抑制作用呈现电压依赖性和可逆性.激光作用可显著影响通道稳态激活和失活过程，激光照射组和对照组比较，电流稳态激活曲线向去极化方向移动，半数激活电压升高，曲线斜率因子改变；而失活曲线向超极化方向移动，半数失活电压降低，曲线斜率因子不变.结果表明，弱激光作用海马神经细胞可以改变其瞬时外向钾离子通道特性，从而影响动作电位的形成和发放，调节神经元生理功能,有利于受损神经元的恢复和再生. 关键词： 瞬时外向钾离子电流 膜片钳技术 弱激光照射 神经元     

THE PROPERTY OF PERIPHERAL INPUT OF PROJECTION NEURONS IN THE SPINAL CORD DORSAL HORN

Antidromic and orthodromic responses of the projection neurons in the dorsal horn ofthe spinal cord have been recorded by a glass microelectrode in anesthetized and paralyz-ed cats. Furthermore, the effect of cervical segment antidromic stimulation to orthodromicresponse of the projection neurons has been observed by way of conditioning-test stimulation. Among all the spinocervical tract neurons (SCT), the dorsal column postsynaptic neu-rons (DCPS) and the spinocervical tract-dorsal column postsynaptic neurons (SCT-DCPS),which were identified by cervical segment antidromic stimulation, 46% are low-thresholdmechanoreceptive (LTM) and 54% are wide-dynamic-range (WDR) neurons. Most LTMneurons can evoke the same response to both 10 times (10 T) and 50 times (50 T) the thresh-old stimulation on the peroneal nerve. Most WDR neurons to 50 T intensity stimulation arestronger than the 10 T stimulation. Under the antidromic-cervical segment conditioningstimulation, the amount of orthodromic-discharging in most WD     

人胶质瘤细胞SWO-3 8神经营养活性物的初步分离

制备了人胶质瘤细胞ＳＷＯ－３８条件培养基（ＳＷＯ－３８ＣＭ）,经超滤浓缩,截留分子量大于１０ｋＤ的ＳＷＯ－３８ＣＭ,并运用快速自动比色微量分析法检测其对体外培养的ＳＤ大鼠小脑皮质神经元的作用,结果表明,分子量大于１０ｋＤ的ＳＷＯ－３８ＣＭ对神经元的作用,与对照组比较有明显的神经营养活性,分子量大于１０ｋＤ的ＳＷＯ－３８ＣＭ经ＳｅｐｈａｃｒｙｌＳ－２００－ＨＲ凝胶层析并结合生物活性鉴定,获得了具有神