甲流病毒传播的年龄结构模型的稳定性

研究甲型H1N1流感病毒的传播规律,建立年龄结构具有接种措施的SEIR流行病模型,给出了疾病流行的阈值并证明了地方病平衡点的稳定性问题.最后根据一些实际数据,进行数值模拟进而对模型的合理性加以完善,借助模型预测下一阶段甲流爆发的可能性并提出相关应对措施.     

Dynamics of an oncolytic virus model

An ODE model for oncolytic virus dynamics with four parameters is discussed. Although it is hard to obtain the explicit expressions of coordinates of equilibria, we find distribution of equilibria qualitatively, and obtain the exact number of equilibria and their qualitative properties. We further give a complete analysis on local bifurcations such as saddle–node, Bogdanov–Takens, and Hopf bifurcations. Resultant elimination is applied to decompose the semi-algebraic varieties of Lyapunov quantities under the restriction of biological meaning, and we show that the weak focus is of order at most 3.     

Carrier Ampholyte Isoelectric Focusing Based Two-Dimensional Electrophoresis in Preliminary Screening of Differential Proteomics Analysis

Two-dimensional electrophoresis is a current method for separating complex protein mixtures of a given sample in different states. In this study an improved carrier ampholyte isoelectric focusing method has been evaluated for its capacity for preliminary screening of expressional proteomics subjects. In comparison with current carrier ampholyte isoelectric focusing, this method showed enough resolution power to display major expressional changes in proteomic samples and demonstrated it can be used as a substitution for the immobiline based isoelectric focusing method.     

介导GAD基因的人泡沫病毒载体的构建及其应用

构建了两种新的重组人泡沫病毒载体pGPSNI—hGAD67和pGPSNI—hGAD65，分别携带人符氨酸脱羧酶（GAD）的两种同工酶GAD65和GAD67基因，并研究其在帕金森病（PD）模刭鼠丘脑底核中的表达及对帕金森病的治疗作用．RT—PCR结果表明，人泡沫病毒载体成功地介导了GAD65基因和GAD67基因在PD模型鼠丘脑底核中有效表达．动物行为检测表明，GAD65基因导入组PD模型鼠行为与对照组相比得到明显的改善（n=12，由〈0．01）．     

猪瘟病毒RT-PCR产物的T载体克隆、测序及同源比较

利用ＴａｑＤＮＡ聚合酶的无模板延伸活性制备了可直接克隆ＰＣＲ产物的Ｔ载体，并成功地对猪瘟病毒ＨＣＬＶ株的ＲＴ－ＰＣＲ产物进行了克隆．ＤＮＡ序列测定和同源性分析表明该ＲＴ－ＰＣＲ产物是猪瘟病毒特异的，与已知序列的猪瘟病毒毒株的序列同源性均高达９６．７％～９８．７％．ＨＣＬＶ株与Ｃ株具有相同的起源，但序列比较发现它们既有共同的点突变，也有序列上的差异，表明这两株病毒在各自独立的传代过程中已发生了各不相同的遗传性变异，值得进一步比较研究．     

分子标记与QTL间连锁检测与估计的方差分析法及其应用

拓展了适用于多种作图群体的检测数量性状基因座位（ＱＴＬ）与分子标记（ＭＬ）间连锁关系的方差分析模型，并提出利用该方差分析之期望均方，在一定的遗传假定前提下，能够用于估计ＭＬ与ＱＴＬ间的重组值．文章最后给出了一个分析实例，将水稻广亲和基因进行了定位．     

计算机蠕虫病毒传播的数学模型分析

建立了一种计算机病毒传播的数学模型,并利用微分方程理论进行了分析,得到了计算机病毒消除的阈值,并进行了数值模拟.     

Microcalorimetric study of virus infection; The effects of hyperthermia and 1b recombinant homo interferon on the infection process of BHK-21 cells by foot and mouth disease virus

The metabolic process of BHK-21 cell line infected by foot and mouth disease virus (FMDV) was determined by using LKB-2277 Bioactivity Monitor. The aim of the present study is to investigate the metabolic thermal power of the virus infection process of BHK-21 cells, the effects of combinational treatments of hyperthermia and 1b recombinant homo interferon on this process. In contrast to the metabolic thermal power of uninfected BHK-21 cells, the thermogenetic curves show that the energy metabolism mechanisms of BHK-21 cells were significantly changed by the virus infection process. The maximum thermal power decreased and the time needed to reach the maximal thermal power increased with the increasing concentration of interferon. The results also show that the infection process was thermosensitive. But no apparent synergetic effect of the combinational treatments of hyperthermia and interferon was observed. The present microcalorimetric results are in accordance with the cytomorphology observations.     

麻疹病毒抗体的酶免疫电化学分析法研究

建立了测定麻疹病毒抗体的酶免疫电化学分析法。其原理是在异相酶联免疫之后用极谱法检测酶促产物。本法采用小型三电极系统“ＤＭＥ－Ｐｔ－Ａｇ／ＡｇＣｌ”直接与商品酶联板配套使用，简化了操作步骤。对测定麻疹抗体的合适条件以及灵敏度，特异性等作了考察。结果表明，本法特异性强，灵敏度高，检测范围宽，不受样品颜色、浊度、杂散光及酶联板自身光吸收不均一的影响；应用于测定三批共１１８份血清样品，阳性率分别为１５％（６／４０），６２．５％（２５／４０）和３１．５８％（１２／３８），用４６份样品对本法和ＥＬＩＳＡ作相关性检验，其相关性良好（γ＝０．７７．ｔ＿ｒ＝１０．８４，Ｐ＜０．００１，相关显著）。