Determination of the configuration of histrelin,and LHRH analog,by chiral capillary gas chromatography

Summary Summary The assigned chirality at each center of the synthetic nonapeptide histrelin (L-pyroglutamyl-L-histidyl-L-tryptophyl-L-seryl-L-tyrosyl-D-N^im-benzyl-histidyl-L-leucyl-L-arginyl-L-proline-ethylamide) was verified using chiral gas chromatography. The procedure involved acid hydrolysis of histrelin to the constituent amino acids, derivatization as the N-pentafluoropropionyl/isopropyl esters and the analysis of the mixture using a commercially available 25m chiral capillary column (Chirasil-L-Val). There was no significant difference in the retention time of the amino acids obtained from the hydrolysate mixture when compared to the appropriate standards. Additionally, the hydrolysate was spiked with the D and L amino acids to prove the identity of closely eluting peaks. Luteinizing hormone-releasing hormone     

In Situ Gold Nanoparticle Gradient Formation in a 3D Meso‐ and Macroporous Polymer Matrix

Herein, the development and characterization of a 3D gradient structure of gold nanoparticles is described. The gradient of gold nanoparticles is made in situ in a macroporous nonionic block copolymer hydrogel matrix, through gold ion diffusion control. The polymer provides a matrix for diffusion of gold ions, acts as a template for controlling nanoparticle growth, and facilitates the in situ reduction of gold ions to gold nanoparticles. A clear gradient in gold nanoparticles is observed across the 3D space of the polymer matrix using scanning electron microscopy, fluorescence microscopy, atomic force microscopy, and thermogravimetric analysis. The particle gradient is further functionalized with both hydrophobic and hydrophilic groups via thiol‐gold linkage to demonstrate the ability to form gradients with different chemical functionalities. Using additive manufacturing, the polymer can also be printed as a porous network with possible applications for 3D cell culturing in, e.g., biomaterials research.     

Versatile Stamps in Microcontact Printing: Transferring Inks by Molecular Recognition and from Ink Reservoirs

Microcontact printing is a heavily used surface modification method in materials and life science applications. This concept article focuses on the development of versatile stamps for microcontact printing that can be used to bind and release inks through molecular recognition or through an ink reservoir, the latter being used for the transfer of heavy inks, such as biomolecules and particles. Conceptually, such stamp properties can be introduced at the stamp surface or by changing the bulk stamp material; both lines of research will be reviewed here. Examples include supramolecular stamps with affinity properties, polymer‐layer‐grafted PDMS stamps, and porous multilayer‐grafted PDMS stamps for the first case, and hydrogel stamps and porous stamps made by phase‐separation micromolding for the second. Potential directions for future advancement of this field are also discussed.     

核苷类似物5-杂环取代或1,5-二杂环取代-1-α/β-D-5-脱氧呋喃核糖的合成

核糖在酸的作用下于甲醇中闭环得到呋喃核糖甲苷, 将其2,3-二羟基形成异亚丙基后, 与对甲苯磺酰氯作用生成5-O-对甲苯磺酰基-2,3-O-异亚丙基-1-α/β-D-呋喃核糖甲苷, 最后与取代哌嗪或吗啉反应, 再在酸的作用下除去2,3-保护基, 得到5-脱氧-5-杂环取代-1-α/β-D-呋喃核糖甲苷. 它们的结构经¹H NMR和MS确证.     

Micro-patterning of vapor-phase polymerized poly(3,4-ethylenedioxythiophene) (PEDOT) using ink-jet printing/soft lithography

Highly conductive and transparent poly(3,4-ethylenedioxythiophene) (PEDOT) thin films can be prepared effectively via vapor-phase polymerization (VPP) with the addition of imidazole (Im) based derivatives. The addition of Im that has one and/or two alkyl substituents significantly improved the electrical conductivity of PEDOT thin films. In an effort to develop a facile PEDOT micro-patterning method, we investigated ink-jet printing and soft lithography. The procedure of oxidant patterning with a weak base followed by VPP of a 3,4-ethylenedioxythiophene (EDOT) monomer provides an effective and simple method for micro-patterning of an intrinsic conductive polymer (ICP).     

Z箍缩驱动器与丝阵负载耦合特性研究

以丝阵内爆零维模型为基础,采用Pspice模拟行为建模方法,建立了丝阵内爆动态电感与Z箍缩驱动器耦合的全电路模型,实现驱动器放电过程与丝阵内爆过程的自洽求解,并研究了丝阵参数、电路参数对内爆过程的影响.结果表明:丝阵负载与驱动器存在强耦合关系,丝阵参数、电路参数对丝阵峰值箍缩电流、内爆时间、内爆动能影响很大;在驱动器参数不变,内爆时间不超过电路固有放电周期1/4的前提下,峰值箍缩电流、内爆时间、内爆动能随丝阵质量的增加而增大,内爆时间随丝阵初始半径的增加而增大;在丝阵参数不变时,随着驱动器等效电容的增大,内爆时间减小,丝阵内爆动能增大,但驱动器储能转化为内爆动能的效率却先增大后减小.对于特定的驱动器,优化的丝阵参数应使内爆过程充分利用驱动器固有放电周期的上升沿,使丝阵快速收缩的时间起点接近电路固有放电周期的四分之一,以获得最大的动能效率. 关键词： Z箍缩驱动器 零维内爆模型 模拟行为建模 耦合特性     

培养学生科学思维和创新能力的研究与实践——谈大学数学研究型教学

通过从一个导数值等式的证明谈起,探讨教师在课堂教学中如何根据教学内容创设能激起学生新异感的问题情景,启发和引导学生发散思考,类比、联想、猜想,探索和发现新问题并给出解答.使学生思维不断攀升,丰富教学内容,激发学生兴趣,培养学生科学思维方法和创新能力.     

Total synthesis of (±)-17-norcamptothecin, a novel E-ring modified camptothecin

The first total synthesis of (±)-17-norcamptothecin, a novel camptothecin analog possessing an α-hydroxy-γ-lactone E-ring, has been accomplished by using a short and flexible route. The stability of this new compound in aqueous medium has been evaluated through fluorescence spectroscopy.     

Synthesis of (sulfonyl)methylphosphonate analogs of prenyl diphosphates

Syntheses of several (sulfonyl)methylphosphonate analogs of geranyl, neryl, and farnesyl diphosphates are described. Key steps include utilization of an (E)-selective Horner-Wadsworth-Emmons olefination which couples an aldehyde to the sulfone phosphonate moiety, and a selective reduction of the resulting dienyl sulfone phosphonate substrates.     

Analog vision chip for motion detection of an approaching object against a moving background based on the insect visual system

A network for motion detection of an approaching object without influence of the moving background was proposed based on the insect visual system. The two-dimensional array of the analog complementary metal oxide semiconductor (CMOS) circuits for extracting the edge signals of the approaching object without outputting those of the moving background was inserted at the first stage of the network. At the next stage, analog CMOS circuits for detection of approaching velocity and direction, which accept the extracted edge signals, were inserted based on the locust vision system. A chip of the proposed network was fabricated with the 1.2 mm CMOS process. It was clarified from the measured results and the simulation results with the simulation program with integrated circuit emphasis (SPICE) that the two-dimensional circuits of the first stage can only generate the signals of target edges. The measured results showed that the chip can detect the approaching velocity and direction of the target in the image which contains the moving background.