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261.
Fan Chen Dr. Baoxin Zhang Dr. Zhenjiang Ding Miao Zhong Dr. Yanan Hou Fang Zhang Dr. Guodong Hu Prof. Jianguo Fang 《Angewandte Chemie (International ed. in English)》2023,62(21):e202301598
The development of small-molecule probes suitable for live-cell applications remains challenging yet highly desirable. We report the first fluorescent probe, RBH, for imaging the heme oxygenase-1 (HO-1) activity in live cells after discovering hemin as a universal dark quencher. Hemin works via a static quenching mechanism and shows high quenching efficiency (>97 %) with fluorophores across a broad spectrum (λex=400–700 nm). The favorable properties of RBH (e.g. long excitation/emission wavelengths, fast response rate and high magnitude of signal increase) enable its use for determining HO-1 activity in complex biological samples. As HO-1 is involved in regulating antioxidant defence, iron homeostasis and gasotransmitter carbon monoxide production, we expect RBH to be a powerful tool for dissecting its functions. Also, the discovery of hemin as a general static dark quencher provides a straightforward strategy for constructing novel fluorescent probes for diverse biological species. 相似文献
262.
Johannes Seibel Giulio Fittolani Hossein Mirhosseini Xu Wu Stephan Rauschenbach Kelvin Anggara Peter H. Seeberger Martina Delbianco Thomas D. Kühne Uta Schlickum Klaus Kern 《Angewandte Chemie (International ed. in English)》2023,62(39):e202305733
Carbohydrates are the most abundant organic material on Earth and the structural “material of choice” in many living systems. Nevertheless, design and engineering of synthetic carbohydrate materials presently lag behind that for protein and nucleic acids. Bottom-up engineering of carbohydrate materials demands an atomic-level understanding of their molecular structures and interactions in condensed phases. Here, high-resolution scanning tunneling microscopy (STM) is used to visualize at submolecular resolution the three-dimensional structure of cellulose oligomers assembled on Au(1111) and the interactions that drive their assembly. The STM imaging, supported by ab initio calculations, reveals the orientation of all glycosidic bonds and pyranose rings in the oligomers, as well as details of intermolecular interactions between the oligomers. By comparing the assembly of D- and L-oligomers, these interactions are shown to be enantioselective, capable of driving spontaneous enantioseparation of cellulose chains from its unnatural enantiomer and promoting the formation of engineered carbohydrate assemblies in the condensed phases. 相似文献
263.
Ria Thielhorn Isabelle Heing-Becker Nadja Hümpfer Jakob Rentsch Rainer Haag Kai Licha Helge Ewers 《Angewandte Chemie (International ed. in English)》2023,62(28):e202302318
Expansion microscopy (ExM) is a recently developed technique that allows for the resolution of structures below the diffraction limit by physically enlarging a hydrogel-embedded facsimile of the biological sample. The target structure is labeled and this label must be retained in a relative position true to the original, smaller state before expansion by linking it into the gel. However, gel formation and digestion lead to a significant loss in target-delivered label, resulting in weak signal. To overcome this problem, we have here developed an agent combining targeting, fluorescent labeling and gel linkage in a single small molecule. Similar approaches in the past have still suffered from significant loss of label. Here we show that this loss is due to insufficient surface grafting of fluorophores into the hydrogel and develop a solution by increasing the amount of target-bound monomers. Overall, we obtain a significant improvement in fluorescence signal retention and our new dye allows the resolution of nuclear pores as ring-like structures, similar to STED microscopy. We furthermore provide mechanistic insight into dye retention in ExM. 相似文献
264.
Chenglong Luan Johanna Angona Arjun Bala Krishnan Manuel Corva Pouya Hosseini Markus Heidelmann Ulrich Hagemann Emmanuel Batsa Tetteh Wolfgang Schuhmann Kristina Tschulik Tong Li 《Angewandte Chemie (International ed. in English)》2023,62(28):e202305982
The role of β-CoOOH crystallographic orientations in catalytic activity for the oxygen evolution reaction (OER) remains elusive. We combine correlative electron backscatter diffraction/scanning electrochemical cell microscopy with X-ray photoelectron spectroscopy, transmission electron microscopy, and atom probe tomography to establish the structure–activity relationships of various faceted β-CoOOH formed on a Co microelectrode under OER conditions. We reveal that ≈6 nm β-CoOOH(01 0), grown on [ 0]-oriented Co, exhibits higher OER activity than ≈3 nm β-CoOOH(10 3) or ≈6 nm β-CoOOH(0006) formed on [02 - and [0001]-oriented Co, respectively. This arises from higher amounts of incorporated hydroxyl ions and more easily reducible CoIII−O sites present in β-CoOOH(01 0) than those in the latter two oxyhydroxide facets. Our correlative multimodal approach shows great promise in linking local activity with atomic-scale details of structure, thickness and composition of active species, which opens opportunities to design pre-catalysts with preferred defects that promote the formation of the most active OER species. 相似文献