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Burel-Deschamps L Mével M Loizeau D Ayadi F Yaouanc JJ Clément JC Jaffrès PA Giamarchi P 《Journal of fluorescence》2008,18(5):835-841
We report here a study on the physicochemical properties of cationic phospholipids liposomes used for lipoplex formulation
and DNA transfer. The original cationic phospholipids synthesized in our laboratory are first presented with the liposome
formulation process. The second part deals with the liposomes fusogenic properties studied by fluorescence resonant energy
transfer (FRET). The nature of the cationic polar head and the formulation with or without a neutral colipid have a great
influence on the FRET signal. The third part reports the study of the viscosity of the liposome by fluorescence anisotropy
measurements. It has been observed that the vectors having a saturated lipid chain exhibit a more pronounced anisotropy than
those having unsaturated lipid chains. Finally, liposomes formed by a mixture of phospholipids and DC-Chol (a rigid lipid)
leads to increase the anisotropy denoting a more rigid liposome. 相似文献
525.
Chin Ken Wong Fan Chen Andreas Walther Martina H. Stenzel 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2019,131(22):7413-7418
Recent years have seen an increased interest in the use of ABC triblock terpolymers to bottom‐up assemble multicompartment patchy nanoparticles. Despite these experimental and theoretical efforts, the applications of polymer‐based patchy nanoparticles remain rather limited. One of the major challenges that eclipses their potential is the lack of knowledge to selectively encapsulate cargoes within different compartments that are separated in the nanometer length scale. Here, strategies are reported to segregate two chemically distinct molecules in either the patches or core compartment of patchy nanoparticles that bear a (bioactive) sugar corona. The potential use of these bioactive patchy nanoparticles containing compartmentalized cargoes for simultaneous drug delivery with real‐time release monitoring capabilities is further demonstrated. 相似文献
526.
Zuhai Lei Caixia Sun Peng Pei Shangfeng Wang Dandan Li Xin Zhang Fan Zhang 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2019,131(24):8250-8255
Small‐molecule organic fluorophores, spectrally active in the 900–1700 nm region, with tunable wavelength and sensing properties are sought‐after for in vivo optical imaging and biosensing. A panel of fluorescent dyes ( CX ) has been developed to meet this challenge. CX dyes exhibit the wavelength tunability of cyanine dyes and have a rigidified polymethine chain to guarantee their stability. They are chemo‐ and photo‐stable in an aqueous environment and have tunable optical properties with maximal absorbing/emitting wavelength at 1089/1140 nm. They show great potential in high‐contrast in vivo bioimaging and multicolor detection with negligible optical cross talk. Förster resonance energy transfer (FRET) between CX dyes was demonstrated in deep tissue, providing an approach for monitoring drug‐induced hepatotoxicity by detection of OONO?. This report presents a series of NIR‐II dyes with promising spectroscopic properties for high‐contrast bioimaging and multiplexed biosensing. 相似文献
527.
通过匹配激光光斑直径与胶体微球的尺寸, 设计制备了银纳米粒子的表面增强拉曼散射(SERS)基底, 并将其用于研究单个银纳米粒子簇的表面增强拉曼光谱. 在制备纳米粒子的过程中, 考察了等离子体刻蚀时间与银沉积厚度对“单”银纳米粒子结构与形貌的影响. 将吡啶、 巯基苯和罗丹明R6G作为SERS探针分子, 研究了其SERS效应, 通过荧光共振能量转移(FRET)机理, 实现了染料分子在单银纳米粒子簇上的SERS效应. SERS光谱测试与相关计算结果表明, 单个银纳米粒子簇的拉曼增强因子能够达到约106. 相似文献
528.
Prasenjit Mondal Rajdeep Chowdhury Somen Nandi Md. Asif Amin Kankan Bhattacharyya Surajit Ghosh 《化学:亚洲杂志》2019,14(24):4616-4624
The dynamics of cell‐cell adhesion are complicated due to complexities in cellular interactions and intra‐membrane interactions. In the present work, we have reconstituted a liposome‐based model system to mimic the cell‐cell adhesion process. Our model liposome system consists of one fluorescein‐tagged and one TRITC (tetramethyl‐rhodamine isothiocyanate)‐tagged liposome, adhered through biotin‐neutravidin interaction. We monitored the adhesion process in liposomes using Förster Resonance Energy Transfer (FRET) between fluorescein (donor) and TRITC (acceptor). Occurrence of FRET is confirmed by the decrease in donor lifetime as well as distinct rise time of the acceptor fluorescence. Interestingly, the acceptor's emission exhibits fluctuations in the range of ≈3±1 s. This may be attributed to structural oscillations associated in two adhered liposomes arising from the flexible nature of biotin‐neutravidin interaction. We have compared the dynamics in a cell‐mimicking liposome system with that in an in vitro live cell system. In the adhered live cell system, we used CPM (7‐diethylamino‐3‐(4‐maleimido‐phenyl)‐4‐methylcoumarin, donor) and nile red (acceptor), which are known to stain the membrane of CHO (Chinese Hamster Ovary) cells. The dynamics of the adhered membranes of two live CHO cells were observed through FRET between CPM and nile red. The acceptor fluorescence intensity exhibits an oscillation in the time‐scale of ≈1±0.75 s, which is faster compared to the reconstituted liposome system, indicating the contributions and involvement of multiple dynamic protein complexes around the cell membrane. This study offers simple reconstituted model systems to understand the complex membrane dynamics using a FRET‐based physical chemistry approach. 相似文献
529.
估算供体-受体对荧光共振能量传递效率的研究 总被引:2,自引:2,他引:0
在有机白光LED的研究中,能量传递效率计算方法的研究是一项很有意义的工作。作者在总结前人工作的基础上,提出了一种利用有机分子发射谱和激发谱估算有机分子荧光共振能量传递(FRET)效率相对值的新方法,这种方法的优点是数据处理过程简单,对实验仪器设备要求不高,并且不需要通常计算方法所需的荧光量子产率等参数,缺点是只能计算能量传递的相对值。利用这种方法计算了甲萘酚、溴甲酚紫、萤光素钠盐三种能量受体和能量供体核黄素在不同浓度下能量传递效率的相对值,计算结果与实验结果基本符合,验证了此方法的合理性。 相似文献
530.
B. Pispisa A. Palleschi C. Mazzuca L. Stella A. Valeri M. Venanzi F. Formaggio C. Toniolo Q. B. Broxterman 《Journal of fluorescence》2002,12(2):213-217
Combination of fluorescence resonance energy transfer (FRET) measurements with molecular mechanics results makes it possible to determine the most relevant structural features of a series of short, ordered L-(Me) Val-based peptides [(Me) Val = C-methylvaline] in methanol solution. 相似文献