High Purity DNA Extraction with a SPE Microfluidic Chip Using KI as the Binding Salt

With the development of the micro electro mechanical system (MEMS) technology, micro total analytical systems (μ-TAS) which has the potential for integrating sample pretreatment, target amplification, and detection, has been in progress. The micro analyt…     

羊栖菜多糖的提取和分离

羊栖菜多糖是从马尾藻科植物羊栖菜（Sargassum fusiforme(Harv.)Setchel)中提取到的，具有抗肿瘤，抗凝血，降血糖，提高机体免疫功能等生理活性，本文研究用水提法提取羊栖菜多糖，考虑到提取温度，时间，加水量等因素的影响，优化提取条件，确定水提法的最佳温度80℃，提取时间3h，水加量为羊栖菜样品的3倍，并采用乙醇沉淀，透析及凝胶层析等方法对羊栖菜提取物中多糖进行沉淀，提取和分离获得4个组分，并用光谱扫描法，凝胶色谱和旋光度法鉴定其纯度，进一步研究其分离获得的组分对生物体免疫功能指标的影响。     

中药土鳖虫溶栓成分的分离纯化研究

对土鳖虫水浸醇沉提取物2号样品进行了离子交换柱层析，得组分Ⅰ、Ⅱ、Ⅲ。实验结果表明，组分Ⅲ的溶栓活性明显高于组分Ⅰ和组分Ⅱ，其蛋白质占量为88．9％，分子量约为38，018，效价为313UK／mg，比活力为352UK／mg蛋白。将组分Ⅲ再进行凝胶过滤柱层析，得组分Ⅳ、Ⅴ、Ⅵ。组分Ⅵ的生物活性高于组分Ⅳ和组分Ⅴ，蛋白质含量为89．3％，电泳呈现两条带，分子量约为34，623和39，811，效价为77UK／mg，比活力为86UK／mg蛋白。将组分Ⅵ进行反相高效液相色谱柱层析，收集保留时间为17min的洗脱峰，得到土鳖虫纯蛋白质，呈白色絮状，极易溶于水。再一次用反相高效液相色谱检查其纯度，保留时间为17．073min．无杂质峰。纤溶活性实验结果表明，组分Ⅵ既有直接降解纤维蛋白的作用，同时也有纤溶酶原激活剂样作用。     

Utility of lab-on-a-chip technology for high-throughput nucleic acid and protein analysis

On-chip electrophoresis can provide size separations of nucleic acids and proteins similar to more traditional slab gel electrophoresis. Lab-on-a-chip (LoaC) systems utilize on-chip electrophoresis in conjunction with sizing calibration, sensitive detection schemes, and sophisticated data analysis to achieve rapid analysis times (<120 s). This work describes the utility of LoaC systems to enable and augment systems biology investigations. RNA quality, as assessed by an RNA integrity number score, is compared to existing quality control (QC) measurements. High-throughput DNA analysis of multiplex PCR samples is used to stratify gene sets for disease discovery. Finally, the applicability of a high-throughput LoaC system for assessing protein purification is demonstrated. The improvements in workflow processes, speed of analysis, data accuracy and reproducibility, and automated data analysis are illustrated.     

Immobilisation of a repressor protein for binding of plasmid DNA

The use of plasmid DNA in gene therapy and genetic vaccination has increased the need for scalable and sustainable production processes. One key challenge for bioprocess engineering is the separation of plasmid DNA from structurally related impurities. Affinity purification procedures allow a highly selective capturing of the target molecule. In this paper, we present the isolation of a his-tagged lac repressor, its non-covalent immobilisation to different matrices and binding of DNA, thus enabling us to screen for combinations of ligands and stationary phases by using a building block principle.     

Preparation and properties of AgInS2 and AgInSe2 Single crystals and of the Quaternary alloys AgInSe2(1−x)S2x

Summary AgInS₂ and AgInSe₂ single crystals have been prepared by chemical transport with iodine and by the Bridgman method. Syntheses of the quaternary alloys AgInSe_2(1−x)S_2x have also been carried out. The splittings of the uppermost valence bands in orthorhombic AgInS₂ have been determined from the photoconductivity spectrum. The lattice parameters, the energy gap, the magnetic suceptibility and the melting point of all the materials are presented. Paper presented at the ?V International Conference on Ternary and Multinary Compounds?, held in Cagliari, September 14–16, 1982.     

Über die Möglichkeit einer strahlenchemischen Reinigung von Abwässern,die Dodezylbenzolsulfonat und Nonylphelylpolyglykoläther enthalten

Es wurde die Möglichkeit des strahlenchemischen Abbaus einiger Detergenzien untersucht, die in Abwässern enthalten sind. Es wurde festgestellt, daß von den oberflächenaktiven Substanzen (OFAS) die den Hauptteil der aktiven Substanz der Detergenzien darstellen, die ionogenen (z. B. Dodezylbenzolsulfonat) bei verhältnismäßig niedrigeren Dosen zersetzt werden als die nichtionogenen (z. B. Nonylphenylpolyglykoläther). Bei gleicher Konzentration beider Stoffe bewirken Dosen in der Größenordnung von 5 bis 6 kGy einen höheren Grad des Abbaus bei Dodezylbenzolsulfonat als Dosen von 10 bis 20 kGy beim Nonylphenylpolyglykoläther. Versuche mit realen Abwässern, die die Präparate ?Biopon”, ?Alkasuper” und ?Kitka” enthalten, bestätigen die erkannte Gesetzmäßigkeit und zeigen die Perspektive eines strahlenchemischen Abbaus für die Reinigung von Abwässern, die ionogenc OFAS enthalten.     

量子信令中继器模型及性能仿真

为了解决量子信令远程传输损伤问题,提出了一种量子信令中继器模型. 在该模型中,首先对接收到的信令量子态进行纠缠纯化和纠缠分发,这样就可在收发双方之间建立纠缠信道. 其次,通过纠缠交换,传送所要发送的量子信令,从而完成量子信令的中继. 仿真结果表明,在保真度为0.98、纠缠对成功建立概率为0.98的条件下, 信令中继成功率可以达到97%以上;在纠缠交换概率、纯化概率和纠缠对成功建立概率都为0.98时, 量子信令中继器的吞吐量可达到183kbps,以上各项指标能够有效满足量子通信对信令中继的要求. 关键词： 量子通信 量子信令 纠缠纯化 纠缠分发     

Crystallization of vitamin C in a continuous DT MSMPR crystallizer – Size independent growth kinetic model approach

The experimental results concerning continuous mass crystallization process in L(+)‐ascorbic acid – water system are presented and discussed. Influence of L(+)‐ascorbic acid concentration in a feeding solution and mean residence time of suspension in laboratory DT MSMPR crystallizer on product crystal size distribution as well as nucleation and growth kinetics were determined. Kinetic parameter values were evaluated on the basis of size–independent growth (SIG) kinetic model (McCabe's ΔL law). It was observed, that within the examined range of crystallizer productivity (120–1600 kg LAA crystals m^–3h^–1) crystal product population of mean size L_m from 0.2 to 0.3 mm and CV from 66.6 to 49% is withdrawn. Linear growth rate values present decreasing trend (from ca. 7 · 10^–8 to ca. 6 · 10^–8 m s^–1) with the productivity increase (assuming constant mean residence time of suspension τ = 900 s). Occurrence of secondary nucleation within the circulated and mixed suspension, resulting from crystal attrition and breakage, was observed. The parameter values in design equation connecting linear growth rate and suspension density with nucleation rate were determined. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)     

Applications of magnetic materials separation in biological nanomedicine

As a result of their advantages for superparamagnetic properties, good biocompatibility, and high binding capacity, functionalized magnetic materials became widely popular over the past couple of decades, being applied on large scale in various processes of sample preparation for biomedicine. In this work, we perform an in‐depth review on the current progress in the field of magnetic bead separation, discussing in detail the physical basis of this process, various synthesis methods and surface modification strategies. We place special focus of attention as well on the latest applications of magnetic polymer microspheres in cell separation, protein purification, immobilized enzyme, nucleic acid separation, and extraction of bioactive compounds with low molecular weight. Existing problems are highlighted and possible trends of magnetic separation techniques for biomedicine in the future are proposed.