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491.
脱镁叶绿酸-a甲酯的合成及对牛血清白蛋白的结合作用 总被引:3,自引:0,他引:3
用荧光光谱和UV-Vis光谱研究了脱镁叶绿酸-a甲酯(Methyl pheo-phorbide-a)与牛血清白蛋白(Bovine Serium Albumin,BSA)的相互结合反应。实验表明叶绿酸-a甲酯与牛血清白蛋白的相互结合作用为单一的静态猝灭过程。在水溶液中脱镁叶绿酸-a甲酯发生自聚,它与蛋白质以表观摩尔比2∶1牢固结合,其结合常数KB=6.7×104 L·mol-1。而在四氢呋喃与水的混合溶液中脱镁叶绿酸-a甲酯以单分子状存在, 其与BSA的结合摩尔比为1∶1。BSA分子与叶绿酸-a甲酯的结合点位为1。根据Frster非辐射能量转移机理,求算了给体(BSA)与受体(脱镁叶绿酸-a甲酯)间距离r=3.50 nm和能量转移效率E=0.39。 相似文献
492.
Interaction of Nicotine and Bovine Serum Albumin 总被引:5,自引:0,他引:5
Nicotine. 3-(l-methyl-2-pyrrolidinyl) pyridine, is a major alkaloid in tobacco products.typically composing l-2% weight of tobacco. So far, there are a 'great deal of papersreporting the effect of nicotine on various biological tissues of animals and humans. Thepharmacological effect of nicotine is a dominant addiction factor for smoking. Since avery large population is frequently exposed to nicotine through the mainstream and/orsidestream of smoking inhalation, the interaction of nicotine wit… 相似文献
493.
494.
We consider the adsorption of bovine serum albumin (BSA) on spherical polyelectrolyte brushes (SPB). The SPB consist of a solid polystyrene core of 100nm diameter onto which linear polyelectrolyte chains (poly(acrylic acid), (PAA)) are grafted. The adsorption of BSA is studied at a pH of 6.1 at different concentrations of added salt and buffer (MES). We observe strong adsorption of BSA onto the SPB despite the effect that the particles as well as the dissolved BSA are charged negatively. The adsorption of BSA is strongest at low salt concentration and decreases drastically with increasing amounts of added salt. The adsorbed protein can be washed out again by raising the ionic strength. The various driving forces for the adsorption are discussed. It is demonstrated that the main driving force is located in the electrostatic interaction of the protein with the brush layer of the particles. All data show that the SPB present a new class of carrier particles whose interaction with proteins can be tuned in a well-defined manner. 相似文献
495.
Gopi Perumal Karthick Govindan Alageswaran Jayaram Sarvesweri Sundaramoorthi Wei-Yu Lin 《中国化学会会志》2023,70(2):145-158
Herein, azadipeptidomimetic molecules were designed and synthesized using a quinoline-histidine-amino acid sequence with terminal amino acid modification. In-silico studies were carried out to evaluate the compound's HIV protease-1 inhibition temperament. In-vitro anti-proliferative possessions of the test compounds were studied by executing a series of experiments, namely DNA binding, bovine serum albumin (BSA) binding capability, and cytotoxicity assay on MCF-7 cells. The molecule 5j exhibited remarkable results in all experiments; it has an excellent DNA binding constant (3.76 × 105), higher serum albumin unbound concentration of 5.96%, and a significant antiproliferative effect on MCF-7 cells with IC50 value 0.31 nM. 相似文献