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排序方式: 共有245条查询结果,搜索用时 15 毫秒
11.
The determination of organophosphorus and carbamate pesticides was carried out using an amperometric transducer based on a robust, polishable and easily mechinable biocomposite. The biocomposite material contains graphite powder, a non-conducting epoxy resin and acetylcholinesterase. The enzyme retains its bioactivity in the rigid epoxy-graphite matric. Measurements were carried out with acetylhiocholine as a substrate. Thiocholine produced by enzymatic hydrolysis was oxidized electrochemically at 70 mV (vs. Ag/AgCl in pH 7.0 buffered solution with 0.1 M phosphate and 0.1 m KCl). The decrease rate of substrate steady-state current after the addition of pesticide was used for evaluation. The method of construction allows for the repetitive use of the electrode. Simple polishing procedures are used to regenerate the bioactive transducer surface. 相似文献
12.
A nickel-catalysed chemically modified electrode is described which seems to represent a convenient compromise between ease of preparation, reproducibility, precision and detection limits for the determination of carbohydrates in biological matrices. 相似文献
13.
《Electroanalysis》2004,16(23):1938-1943
An organically modified sol‐gel glass (ORMOSIL) encapsulating pyrroloquinoline quinone (PQQ)‐modified electrode for the rapid, sensitive and simple determination of thiol‐containing compounds such as cysteine and glutathione is reported. The effect of applied potential, nature of thiol compound and pH on the response of the sensor was examined and optimum conditions were determined. The electrochemical responses and detection limits were found to be sensitive to the nature of thiols and pH. The electrochemical responses for cysteine and glutathione at an applied potential of ?0.2 V (vs. Ag/AgCl) were found to be linear with detection limits of 18 nM for cysteine and 36 nM for glutathione at pH 3.5, whereas the detection limits at pH 8.5 were 0.5 μM for cysteine and 1 μM for glutathione. The electrode retained 95% of the original response for 7 days when stored at 4 °C. The ORMOSIL‐encapsulated PQQ was also characterized by spectrophotometry. The absorbance measurement using 5,5′‐dithiobis(2‐nitrobenzoic acid) at 412 nm justify the PQQ‐mediated oxidation of glutathione whereas fluorescence measurements (excitation wavelength=380 nm; emission wavelength=480 nm) justify the successful encapsulation of PQQ in ORMOSIL matrix. 相似文献
14.
This paper presents a simple, rapid and reproducible method of analysis of salbutamol in pharmaceutical products, utilizing batch injection analysis (BIA) associated with amperometric detection. A study of salbutamol oxidation demonstrated a strong dependence between electrode fouling and pH. All determinations were done utilizing a glassy carbon electrode in presence of 3.0 mol l−1 NaOH. A large linear dynamic range from 8×10−7 to 2×10−4 mol l−1 was obtained by using an injected volume of 100 μl with a detection limit of 2.5×10−7 mol l−1. R.S.D. of 0.92% for 50 successive injections of 4×10−6 mol l−1 of salbutamol and a sample throughput of 60 samples per hour were achieved. The method was applied for salbutamol quantification in syrups. 相似文献
15.
An amperometric cholesterol biosensor based on immobilization of cholesterol oxidase in a Prussian blue (PB)/polypyrrole (PPy) composite film on the surface of a glassy carbon electrode was fabricated. Hydrogen peroxide produced by the enzymatic reaction was catalytically reduced on the PB film electrode at 0 V with a sensitivity of 39 μA (mol/L)?1. Cholesterol in the concentration range of 10?5 ? 10?4 mol/L was determined with a detection limit of 6 × 10?7 mol/L by amperometric method. Normal coexisting compounds in the bio‐samples such as ascorbic acid and uric acid do not interfere with the determination. The excellent properties of the sensor in sensitivity and selectivity are attributed to the PB/PPy layer modified on the sensor. 相似文献
16.
The carbon ceramic electrode prepared with sol-gel technique is modified by a thin film of chlorogenic acid (CGA). By immersing the carbon ceramic electrode in aqueous solution of chlorogenic acid at less than 2 s a thin film of chlorogenic acid adsorbed strongly and irreversibly on the surface of electrode. The cyclic voltammetry of the resulting modified CCE prepared at optimum conditions shows a well-defined stable reversible redox couple due to hydroquinone/quinone system in both acidic and basic solutions. The modified electrode showed excellent electrocatalytic activity toward NADH oxidation and it also showed a high analytical performance for amperometric detection of NADH. The catalytic rate constant of the modified carbon ceramic electrode for the oxidation of NADH is determined by cyclic voltammetry measurement. Under the optimised conditions the calibration curve is linear in the concentration range 1-120 μm. The detection limit (S/N = 3) and sensitivity are 0.2 μM and 25 nA μM−1.The results of six successive measurement-regeneration cycles show relative standard deviations of 2.5% for electrolyte solution containing 1 mM NADH, indicating that the electrode renewal gives a good reproducible and antifouling surface. The advantages of this amperometric detector are: high sensitivity, excellent catalytic activity, short response time t < 2 s, remarkable long-term stability, simplicity of preparation at short time and good reproducibility. 相似文献
17.
《Electroanalysis》2004,16(10):827-831
An amperometric detector for hydrophobic ions based on a plasticized poly(vinyl) chloride (PVC) membrane incorporated in a flow‐injection system was developed. A four‐electrode potentiostat with ohmic drop compensation was used, while a flow‐through cell incorporated the four electrodes and the membrane, which contained tetrabutylammonium tetraphenylborate. When the influence of the applied potential and of the flow‐injection variables on the determination of tetrabutylammonium was studied, a linear relationship was observed between current peak height and ion concentration over a range of 5×10?6–6×10?5 M tetrabutylammonium. Good repeatability and between‐day reproducibility and high sample frequency were obtained. The effect of other ions was studied. Two different amperometric methods, indirect and direct, were also developed for the determination of dodecylsulfate in the concentration range 3×10?5–9×10?4 M. 相似文献
18.
Self-assembled monolayer gold electrode for surfactant analysis 总被引:2,自引:0,他引:2
Marc Gerlache Zühre Senturk Guy Quarin Jean-Michel Kauffmann 《Journal of Solid State Electrochemistry》1997,1(2):155-160
A gold electrode coated with a self-assembled monolayer of octane-thiol (SAM/Au) has been used as an amperometric detector
for the determination of surfactants. This detector operated in the presence of a high percentage of organic solvent and was
adapted to an HPLC System. At the SAM/Au, the electrochemical response of an electroactive tracer (potassium ferricyanide)
was completely inhibited, but, in the presence of a cationic surfactant, the electrochemical reduction was progressively restored.
In flow injection analysis, using the SAM/Au in an amperometric flow-through detector polarised at 0.0 V vs Ag/AgCl, a linear
response (i=f{[surfactant]}) was observed for cationic surfactants e.g. cetylpyridinium chloride in the concentration range 2 × 10−6–1 × 10−3 M. The electrochemical data along with the determination of the ion pair stoichiometry between the redox tracer and the surfactant
suggest an electrochemical response related to ion pair formation and governed by electron transfer by tunneling effect.
Received: 28 January 1997 / Accepted: 7 March 1997 相似文献
19.
A Variety of potential–time waveforms are useful in pulsed electrochemical detection (PED) when applied for the amperometric detection of numerous polar organic compounds following their separation by liquid chromatography (LC). Here, we compare the waveforms for pulsed amperometric detection (PAD) and integrated voltammetric detection (IVD) applied for detection of organosulfur compounds at Au electrodes in acidic media. In PAD waveforms, electrodes response is measured at a constant detection potentials. In IVD waveforms, electrodes current is integrated throughout a fast cyclic scan of the detection potential. As a consequence of this difference in detection strategy, the background signal for IVD is significantly smaller for PAD in the detection of organosulfur compounds whose response mechanisms require the concomitant formation of surface oxides on Au electrodes. Furthermore, in comparison to Pad, IVD has a larger sensitivity and a diminished system peak from 02 dissolved in the sample. Use of a preadsorption step increases detection sensitivity in both PAD and IVD. The limit of detection (S/N=3)for cysteine in LC-IVD is ca. 6 nM for a 50-μl injection (i.e., 300 fmol) using a detection waveform that includes a 1000-ms preadsorption period. 相似文献
20.
Acetylcholinesterase was immobilised on magnetic particles and integrated in a flow-injection system via a magnetic reactor. Enzyme activity was determined amperometrically using acetylthiocholine chloride as enzyme substrate. This system was applied to enzyme inhibition tests. Inhibition constants and lower detection limits were determined for carbofuran, paraoxon, malaoxon and paraoxon-methyl. The resulting data were compared to those obtained with a photometric test, i.e. the determination of thiocholine via its reaction with the Ellman's reagent 5,5'-dithio-bis-2-nitrobenzoic acid using the same flow system. As they correlated well to those reported for the native enzyme the flow-injection analysis device can be applied to automated determination and characterisation of enzyme inhibitors. 相似文献