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101.
Immunosensors and biochemical array detection systems based on electrochemical transducers have many advantages such as low detection limit, fast response, simple design and ease of miniaturization. However, further development of such sensors will depend on the availability of suitable substrates that can be converted by a labeling enzyme to an electrochemically active product. Here, we report the synthesis of 4-amino-1-naphthylphosphate and it’s application as a new substrate for alkaline phosphatase. The electrochemical and enzymatic properties of this compound were investigated and compared with the properties of other aromatic 1,4-dihydroxy and 1,4-hydroxy-amine derivatives. The product of the enzyme reaction was 4-aminonaphthol, which was rapidly converted in the presences of air to 1,4-iminonaphthoquinone. This compound could then be detected in an amperometric flow injection assay (AFIA) with −200 mV versus Ag/AgCl potential application. The analytical range for mouse IgG, in an alkaline phosphatase amplified sandwich immuoassay with amperometric detection, was 0.01-100 μg ml−1. 相似文献
102.
A novel HPLC-based method for direct separation of the three isomers of mono-acid diacylglycerols (DAGs), i.e., 1,2-DAG, 2,3-DAG and 1,3-DAG, has been established. The method employs a tandem column system, in which two different columns (a conventional silica gel column and a chiral stationary phase column) are connected in series. Two isomeric mixtures of DAGs (i.e., dicapryloylglycerol and dioleoylglycerol) and lipase-catalyzed reaction mixtures were successfully resolved on the tandem column HPLC system without any derivatization prior to the analysis. According to the established analytical method, stereoselectivity of two lipases toward mono-acid triacylglycerols in ethanolysis reaction was investigated. The tested enzymes were immobilized Candida antarctica lipase B (CALB) and Rhizomucor miehei lipase (RML). Analyses of the enantiomeric purity of 1,2-DAG and 2,3-DAG, generated as intermediates during the reaction, revealed that CALB and RML have sn-3 and sn-1 stereopreference, respectively. 相似文献
103.
Sumiko Suzuki Mika Shirao Momoyo Aizawa Hiroyuki Nakazawa Katsushi Sasa Hideo Sasagawa 《Journal of chromatography. A》1994,680(2):541-547
A method for the determination of synthetic tar dyes used as food additives using capillary electrophoresis with photodiode-array detection was investigated. The dyes Erythrosine (R-3), Phloxine (R-104), Rose Bengal (R-105), Acid Red (R-106), Amaranth (R-2), New Coccine (R-102) and Allura Red AC (R-40) were separated on a capillary column (50 cm × 75 μm I.D.) and identified from the absorbance spectra of each peak. The electrophoresis buffer used was a mixture of 25 mM sodium phosphate buffer and 25 mM sodium borate buffer (1:1) (pH 8.0) containing 10 mM sodium dodecyl sulfate (SDS). Substitution of β-cyclodextrin for SDS in the electrolyte buffer was effective for the separation of R-2 and R-102. This modified method could be employed as an additional assay method for these two dyes. 相似文献
104.
Toichiro A. Katsumi Katsuo Aizawa Yukari Kuroiwa Kouichi Saito Yasushi Kurata Yoshiteru Li Tetsuya Okunaka Chimori Konaka Harubumi Kato 《Photochemistry and photobiology》1996,64(4):671-675
Abstract— The authors performed photodynamic therapy (PDT), avoiding any hyperthermic effects, using a newly developed diode laser and photosensitizer, mono-L-aspar-tyl chlorin e6 (NPe6), of Meth-A fibrosarcoma implanted in mice and achieved tumor therapeutic benefit. The photodynamic light treatment was performed 5 h following the photosensitizer administration. With 5.0 mg/kg NPe6 and light doses of 50, 100, 150 and 200 J/cm2 , the tumor cure rates were 20, 50, 70 and 90%, respectively. With 100 J/cm2 laser exposure and NPe6 doses of 1.25, 2.5, 5.0, 7.5 and 10.0 mg/kg, the tumor cure rates were 0, 20, 50, 70 and 90%, respectively. A charge-coupled device (CCD) camera system was employed to measure the NPe6 fluorescence intensity correlating with the residual amount of the photosensitizer at deferent depth from the tumor surface. The ratios of the NPe6 fluorescence intensity at 3 mm from the tumor surface following 50, 100, 150 and 200 J/cm2 laser exposure to no laser exposure were 0.73, 0.36, 0.22 and 0.16, respectively. With samples sectioned at 1 mm depth, after 50 J/cm2 and the same photosensitizer dose (5 mg/kg) this ratio was 0.19. These results suggest that a certain increase in the tumor tissue level of NPe6 and a certain increase of laser light dose reaching deeper layers of tumor caused an increase in percent cure. In addition, the effectiveness of PDT depends on the total laser dose reaching deeper layers of tumors. Furthermore, the effectiveness of PDT tends to correlate with the amount of NPe6 photobleaching by PDT. 相似文献
105.
A specific sensor for a tumor antigen, α-fetoprotein (AFP) can be prepared from a membrane with immobilized antibody and an oxygen probe with a permeable teflon membrane. Anti-AFP antibody is covalently immobilized on a membrane prepared from cellulose triacetate, 1,8-diamino-4-aminomethyloctane and glutaraldehyde. The sensor is applied to enzyme immunoassay based on competitive antigen-antibody reaction with catalase-labelled antigen. After competitive binding of free and catalase-labelled AFP, the sensor is examined for catalase activity by amperometric measurement after addition of hydrogen peroxide. AFP can be determined in the range 10-11–10-8 g ml-1. 相似文献
106.
The principles of biocatalytic and bioaffinity biosensors are reviewed with emphasis on electron transfer-type enzyme sensors, optical enzyme sensors and optical immunosensors for homogeneous immunoassay. An enzyme sensor for ethanol was fabricated by electrochemical polymerization of pyrrole onto the surface of platinized platinum-adsorbed alcohol dehydrogenase—NAD—Meldola Blue. Ethanol was determined amperometrically by measuring the oxidative current through polypyrrole. An optical enzyme sensor is exemplified by an acethylcholine sensor based on an optical pH fibre sensor using a thin polyaniline film. The optical immunosensor for homogeneous immunoassay consists of an optical fibre, the end of the which is coated with an optically transparent platinum electrode. With using luminol as a label, highly sensitive homogeneous immunoassay is carried out by measuring the electrochemical luminescence of the label. 相似文献
107.
T Yoshioka Y Aizawa T Fujita K Nakamura K Sasahara H Kuwano T Kinoshita H Horikoshi 《Chemical & pharmaceutical bulletin》1991,39(8):2124-2125
The glucuronide of a new oral antidiabetic agent, (+/-)-5-[4-(6-hydroxy-2,5,7,8-tetramethylchroman-2-yl- methoxy)benzyl]thiazolidine-2,4-dione (CS-045) (1), was synthesized to confirm the structure of a metabolite in monkeys (Macaca fascicularis) and to examine its antidiabetic activity. The glucuronide also had antidiabetic activity in KK-mice. 相似文献
108.
Junichi Kinoshita Hitoshi Aizawa Akira Takamori Kazuhisa Yamamoto Hiroshi Murata Koji Tojo 《Optical Review》2016,23(1):121-132
Angular dependence of speckle contrast of speckle pattern projected out of a multi-mode fiber connected to a high-power blue laser module is investigated. The laser module has nine high-power InGaN/GaN blue laser diodes arranged in a three-by-three array. Each of the arrayed laser diodes have slightly different incident angle to the fiber. We have successfully extracted the fine screen speckle pattern from the projected pattern mixed up with the coarse fiber speckle pattern by processing the measured data. It is found that the speckle contrast of the both screen and fiber speckles are larger around the center area where the guided-light component closest to the paraxial direction is projected. This is because the output of the center laser in the array is likely to couple into the paraxial mode more than the rest. Speckle contrast behaviors when applying the speckle reduction methods, fiber vibration, diffuser, and spinning diffuser are also investigated. 相似文献
109.
Kazuyoshi Kanamori Mamoru Aizawa Kazuki Nakanishi Teiichi Hanada 《Journal of Sol-Gel Science and Technology》2008,48(1-2):172-181
Novel aerogels and xerogels with methylsilsesquioxane (MSQ, CH3SiO1.5) networks have been prepared by a modified sol–gel process using surfactant and urea as a phase-separation inhibitor and as an accelerator for the condensation reaction, respectively. Optimized aerogels dried under a supercritical condition not only showed the similar properties as conventional pure silica aerogels such as high transparency and porosity etc, but also demonstrated outstanding mechanical strength against compression; the aerogel drastically shrank upon loading and then recovered when unloaded, which is called a “spring-back” behavior. On ambient pressure drying, the wet gel also exhibited the similar response against compression stress originated from the capillary pressure, and thus xerogels with the comparative structure and properties to those of corresponding aerogels have also been obtained. This unusual mechanical behavior is attributed to the trifunctional flexible networks of MSQ, low silanol concentration which prevents the irreversible shrinkage, and high concentration of a hydrophobic methyl group directly attached to every silicon atom which helps re-expansion after the temporal shrinkage. 相似文献
110.
Bungo Ochiai Yugo Hatano Takeshi Endo 《Journal of polymer science. Part A, Polymer chemistry》2009,47(12):3170-3176
The radical polymerization of glycidyl methacrylate (GMA) was conducted under a carbon dioxide atmosphere (1 atm) in the presence of catalysts for the reaction of carbon dioxide and the oxirane group to afford the five‐membered cyclic carbonate group. The degrees of the carbon dioxide fixation depended on catalysts, concentration, and solvents. In solution reaction, the slower polymerizations resulted in faster carbon dioxide fixation, due to the faster carbon dioxide fixation to GMA than to oxirane moieties in polymers. When the polymerization was conducted in 1,4‐dioxane, which is a good solvent for polyGMA but a poor solvent for the analogous polymer bearing cyclic carbonate moieties, the resulting polymers were precipitated out as the progress of the polymerization and the carbon dioxide fixation. As a result, polymers could be isolated by simple filtration and rinsing with methanol. © 2009 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 47: 3170–3176, 2009 相似文献