全文获取类型
收费全文 | 112219篇 |
免费 | 1610篇 |
国内免费 | 634篇 |
专业分类
化学 | 48596篇 |
晶体学 | 1040篇 |
力学 | 7370篇 |
综合类 | 1篇 |
数学 | 37786篇 |
物理学 | 19670篇 |
出版年
2023年 | 163篇 |
2021年 | 434篇 |
2020年 | 614篇 |
2019年 | 614篇 |
2018年 | 10718篇 |
2017年 | 10528篇 |
2016年 | 6924篇 |
2015年 | 1664篇 |
2014年 | 1302篇 |
2013年 | 2083篇 |
2012年 | 5928篇 |
2011年 | 12789篇 |
2010年 | 6861篇 |
2009年 | 7116篇 |
2008年 | 8791篇 |
2007年 | 10699篇 |
2006年 | 2359篇 |
2005年 | 3298篇 |
2004年 | 3218篇 |
2003年 | 3344篇 |
2002年 | 2354篇 |
2001年 | 761篇 |
2000年 | 779篇 |
1999年 | 568篇 |
1998年 | 602篇 |
1997年 | 564篇 |
1996年 | 590篇 |
1995年 | 416篇 |
1994年 | 444篇 |
1993年 | 430篇 |
1992年 | 355篇 |
1991年 | 343篇 |
1990年 | 305篇 |
1989年 | 281篇 |
1988年 | 286篇 |
1987年 | 298篇 |
1986年 | 238篇 |
1985年 | 441篇 |
1984年 | 390篇 |
1983年 | 333篇 |
1982年 | 404篇 |
1981年 | 339篇 |
1980年 | 355篇 |
1979年 | 312篇 |
1978年 | 275篇 |
1977年 | 261篇 |
1976年 | 234篇 |
1975年 | 222篇 |
1974年 | 197篇 |
1973年 | 169篇 |
排序方式: 共有10000条查询结果,搜索用时 239 毫秒
991.
CE conditions for monitoring the unsaturated disaccharides of hyaluronic acid (di-HA) and chondroitin sulfate (di-CS) using an alkaline tetraborate buffer, electrokinetic sample injection, and UV absorption detection at 232 nm are reported. Separations were performed in an uncoated fused-silica capillary having reversed polarity and reversed electroosmosis generated with the addition of CTAB to the buffer. The influence of various separation parameters, including the concentration of CTAB, buffer pH, concentration of tetraborate, and applied voltage, on the resolution of the two disaccharides was investigated. Baseline separation was obtained with 25 mM tetraborate at pH 10.0 and having 0.05 mM CTAB. Chloride and phosphate in the sample are beneficial for the stacking of the disaccharides, with di-HA forming a much sharper peak than di-CS. Using samples prepared in 25 mM Tris-HCl (pH 7.5) and electrokinetic injection at the cathode at -10 kV for 40 s, linear relationships between the corrected peak area and the concentration of the disaccharides have been found in the ranges of 1.0-400.0 and 0.1-1.0 microg/mL (0.2-1.0 microg/mL for di-CS), with correlation coefficients being >0.9933 in all cases. The RSDs of detection times and corrected peak areas were between 1.13-1.24 and 1.57-2.13%, respectively. Applied to human serum samples that were prepared by ethanol precipitation and depolymerization of the two polysaccharides with chondroitinase ABC reveals comigration of endogenous compounds with di-HA and a sample-dependent detection time. The di-HA content in the serum sample can be estimated via subtraction of the blank peak that is obtained without enzymatic hydrolysis. 相似文献
992.
Vaze A Parizo M Rusling JF 《Langmuir : the ACS journal of surfaces and colloids》2004,20(25):10943-10948
Redox proteins attached to surfaces designed for biocatalysis hold promise for future clean synthetic routes. It is advantageous for these biocatalysts to operate in low-toxicity fluids with a high capacity to dissolve reactants. Here we report cross-linked films of myoglobin (Mb) and poly(L-lysine) (PLL) chemically attached to oxidized carbon cloth cathodes that in microemulsions feature the protein in a water-rich film environment with reactant in an oil-rich environment. These cross-linked Mb/PLL films were the most stable in microemuslions and had the largest turnover rates for epoxidation of styrene compared to lightly cross-linked or uncross-linked Mb/poly(styrene sulfonate) films. Up to 40-fold larger turnover rates were found in bicontinuous microemulsions compared to oil-in-water microemulsions and micelles. Enhanced turnover rates are correlated with up to 10-fold faster mass transport of solutes in the oil phases of the bicontinuous fluids. 相似文献
993.
994.
995.
Dendritic molecular capsules for hydrophobic compounds 总被引:1,自引:0,他引:1
Morgan MT Carnahan MA Immoos CE Ribeiro AA Finkelstein S Lee SJ Grinstaff MW 《Journal of the American Chemical Society》2003,125(50):15485-15489
Reichardt's dye, a highly solvatochromic dye, was encapsulated within poly (glycerol succinic acid) ([Gn]-PGLSA-OH) dendrimers to investigate the interior environment of these dendritic macromolecules. The absorption maximum for the encapsulated Reichardt's dye in water was indicative of a relatively high dielectric constant present within the dye/dendrimer complex. (1)H NMR of the encapsulated complex showed the presence of aromatic protons from Reichardt's dye along with the aliphatic protons of the dendrimer. Additionally, there were substantial changes in T(1) and T(2) times of the encapsulated dye when compared with the free dye, and (1)H NOESY spectra for the complex showed a significant number of intermolecular NOE cross-peaks. These data reveal the close through-space proximity of the dye to the dendrimer and the restricted motion of the encapsulated dye. To demonstrate the potential use of these macromolecules as drug delivery vehicles, the poorly water-soluble anticancer drug 10-hydroxycamptothecin (10HCPT) was encapsulated within a carboxylated PGLSA dendrimer ([G4]-PGLSA-COONa). Cytotoxicity assays with human breast cancer cells showed a significant reduction of cell viability, demonstrating that 10HCPT retains activity upon encapsulation. 相似文献
996.
Microchimica Acta - The techniques for investigating the chemistry of radioactive elements usingμg quantities of material are described. These include methods of transfer and carrying out... 相似文献
997.
The Michael addition of thiols to enones is reported as a new method for dynamic combinatorial library synthesis. 相似文献
998.
Phase separation in the formation of hollow particles by suspension polymerization for divinylbenzene/toluene droplets dissolving polystyrene 总被引:1,自引:0,他引:1
Phase-separation behavior within polymerizing divinylbenzene/toluene droplet dissolving polystyrenes (PS) was investigated to clarify the formation mechanism of the hollow polymer particles by suspension polymerization. No hollow particles were obtained at a low content of low-molecular-weight PS where phase separation occurred at high conversion. On the other hand, hollow particles were obtained at a high content of high-molecular-weight PS where phase separation occurred at low conversion. The phase separation in an early stage of the polymerization, which was promoted by the presence of PS and cross-links of polydivinylbenzene, was a key factor for the formation of the hollow structure. 相似文献
999.
Ningyi Chen Jiayi Xu Michael Reuman Xun Li Ronald K. Russell Zhihua Sui 《Tetrahedron letters》2006,47(33):5909-5913
Various substituents were introduced onto the methyl group in 4-methyl coumarins through lithiation, followed by reactions with a wide range of electrophiles. The presence of an alkoxy group on 6′-phenyl ring was found to be pivotal for the success of this reaction. This procedure provided a convenient synthetic pathway to elaborate the methyl group of 4-methylcoumarins. Application of this methodology was showcased with the synthesis of biologically important novel tetracyclic chromene ring systems (n = 1-3). 相似文献
1000.
An unusual infrared chemiluminescence emission (8130Å) of methylene blue, and other thiazine dyes, sensitized by singlet molecular oxygen is reported. This chemiluminescence does not correspond to the ordinary fluorescence of the dye and cannot be explained by previously proposed mechanisms for singlet oxygen sensitized emissions of dyes. From energetic considerations singlet molecular oxygen in its 1Σg+ state is postulated as the sensitizing agent for the thiazine dye chemiluminescences. Schemes in which 1Σg+ oxygen transfers electronic excitation energy (a) to the lowest triplet state of the dye, (b) to a combined multiplicity state of the lowest triplet state of the dye, and triplet molecular oxygen, or (c) to a charge-transfer state between the dye and oxygen, are compared. The chemiluminescence of methylene blue in aqueous solution may be used as a luminescent probe for 1Σg+ oxygen. 相似文献