A Novel Copper Oxide Nanoparticle Conjugated by Thiosemicarbazone Promote Apoptosis in Human Breast Cancer Cell Line

The current study highlights the apoptotic activity of copper oxide (CuO) nanoparticles functionalized by Glutamic acid and conjugated by thiosemicarbazone (TSC) toward human breast cancer (MCF-7) and normal (HEK293) cell lines. To this aim, the co-precipitation method was used for preparation the CuO nanoparticles. Afterward, the CuO nanoparticles functionalized by glutamic acid. After that the functionalized copper oxide nanoparticles (CuO@Glu) conjugated to thiosemicarbazone. In next step, the final nanoparticle product (CuO@Glu/TSC) was characterized by physico-chemical techniques including Fourier transform infrared (FT-IR) spectroscopy, Scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), Energy dispersive X-ray analysis (EDX), zeta potential analysis and dynamic light scattering (DLS). The effects of in vitro cell viability in CuO@Glu/TSC nanoparticles showed the anti-proliferative properties with a dose-dependent manner (IC₅₀?=?133.97 µg/ml). The IC₅₀ of CuO@Glu/TSC on normal cell line was 230.2 µg/ml. This IC₅₀ deference shows high cytotoxicity of CuO@Glu/TSC nanoparticles on tumor cells and low cytotoxicity on non-tumorigenic cells (HEK293) and is considered as an important aspect for this nanoparticles. Also, CuO@Glu/TSC nanoparticles had efficient effects in inhibiting the growth of breast cancer cell line (MCF-7). In addition, the CuO@Glu/TSC nanoparticle induced apoptosis symptoms which were assessed by Caspase-3 activation assay, Annexin V/ propidium iodide flow cytometry, and Hoechst 33258 staining. Further, Bax and Bcl-2 genes expression was estimated by real time PCR. The expression of Bax increased 1.69 fold, while the expression of Bcl-2 decreased 0.6 fold. The results of the current study propose that CuO@Glu/TSC nanoparticles reveal effective anti-cancer activity against breast cancer cell line.

     

Effect of initial sol concentration on the microstructure and morphology of carbon aerogels

Journal of Sol-Gel Science and Technology - New carbon aerogels were prepared using Novolac sol as precursor. The elaboration process and the structural characterizations of these porous carbon...     

Copper(I)–creatine complex on magnetic nanoparticles as a green catalyst for N- and O-arylation in deep eutectic solvent

Immobilization of copper(I) ions on magnetic nanoparticles was performed using surface modification of Fe₃O₄ with creatine. Fe₃O₄@creatine-Cu(I) magnetic catalyst was synthesized and applied in C&bond;X cross-coupling reactions with aryl halides in a deep eutectic as a green solvent. The results indicate the Fe₃O₄@creatine-Cu(I) magnetic nanoparticles showed excellent activity and high stability. In addition, it was revealed that this catalyst can be recycled five times without significant loss in catalytic activity.     

A novel MAV with treadmill motion of wing

The Magnus effect is well known phenomena for producing high lift values from spinning symmetrical geometries such as cylinders, spheres, or disks. But, the Magnus force may also be produced by treadmill motion of aerodynamic bodies. To accomplish this, the skin of aerodynamic bodies may circulate with a constant circumferential speed. Here, a novel wing with treadmill motion of skin is introduced which may generate lift at zero air speeds. The new wing may lead to micro aerial vehicle configurations for vertical take-off or landing. To prove the concept, the NACA0015 aerofoil section with circulating skin is computationally investigated. Two cases of stationary air and moving air are studied. It is observed that lift can be generated in stationary air although drag force is also high. For moving air, the lift and drag forces may be adopted between the incidence angles 20° to 25° where lift can posses high values and drag can remain moderate.     

The effects of chemical kinetics and wall temperature on performance of porous media burners

This paper reports a two-dimensional numerical prediction of premixed methane-air combustion in inert porous media burner by using of four multi-step mechanisms: GRI-3.0 mechanism, GRI-2.11 mechanism and the skeletal and 17 Species mechanisms. The effects of these models on temperature, chemical species and pollutant emissions are studied. A two-dimensional axisymmetric model for premixed methane-air combustion in porous media burner has developed. The finite volume method has used to solve the governing equations of methane-air combustion in inert porous media burner. The results indicate that the present four models have the same accuracy in predicting temperature profiles and the difference between these profiles is not more than 2 %. In addition, the Gri-3.0 mechanism shows the best prediction of NO emission in comparison with experimental data. The 17 Species mechanism shows good agreement in prediction of temperature and pollutant emissions with GRI-3.0, GRI-2.11 and the skeletal mechanisms. Also the effects of wall temperature on the gas temperature and mass fraction of species such as NO and CH₄ are studied.     

Simulation of quantum-well slipping effect on optical bandwidth in transistor laser

An optical bandwidth analysis of a quantum-well （16 nm） transistor laser with 150-μm cavity length using a charge control model is reported in order to modify the quantum-well location through the base region. At constant bias current, the simulation shows significant enhancement in optical bandwidth due to moving the quantum well in the direction of collector-base junction. No remarkable resonance peak, limiting factor in laser diodes, is observed during this modification in transistor laser structure. The method can be utilized for transistor laser structure design.     

On dual Bernstein polynomials and stochastic fractional integro-differential equations

In recent years, random functional or stochastic equations have been reported in a large class of problems. In many cases, an exact analytical solution of such equations is not available and, therefore, is of great importance to obtain their numerical approximation. This study presents a numerical technique based on Bernstein operational matrices for a family of stochastic fractional integro-differential equations (SFIDE) by means of the trapezoidal rule. A relevant feature of this method is the conversion of the SFIDE into a linear system of algebraic equations that can be analyzed by numerical methods. An upper error bound, the convergence, and error analysis of the scheme are investigated. Three examples illustrate the accuracy and performance of the technique.     

Noise analysis of coaxial Schottky barrier carbon nanotube fets using non equilibrium Green’s function formalism

The effect of noise on the performance of Schottky Barrier Carbon Nanotube Field Effect Transistors (SB-CNTFETs) has been investigated under various bias conditions. In order to calculate the noise power spectral density, the Non-Equilibrium Green’s Function formalism (NEGF) is used to obtain the transmission coefficient and the number of carriers inside the channel. Results are presented in two sections: In the first section the Hooge’s empirical rule is used to investigate the flicker noise properties of SB-CNTFETs with defects in the gate oxide region, while in the second section the thermal and shot noise properties of SB-CNTFETs are studied. Finally, the best bias points in the ON and OFF states have been suggested according to the total noise power spectral density and the device signal to noise ratio.      

Evaluation of antiviral activities of curcumin derivatives against HSV-1 in Vero cell line

Antiviral drug resistance is one of the most common problems in medicine, and, therefore, finding new antiviral agents, especially from natural resources, seems to be necessary. This study was designed to assay the antiviral activity of curcumin and its new derivatives like gallium-curcumin and Cu-curcumin on replication of HSV-1 in cell culture. The research was performed as an in vitro study in which the antiviral activity of different concentrations of three substances including curcumin, Gallium-curcumin and Cu-curcumin were tested on HSV-1. The cytotoxicity of the tested compounds was also evaluated on the Vero cell line. The CC50 values for curcumin, gallium-curcumin and Cu-curcumin were 484.2 microg/mL, 255.8 microg/mL and 326.6 microg/mL, respectively, and the respective IC50 values 33.0 microg/mL, 13.9 microg/mL and 23.1 microg/mL. The calculated SI values were 14.6, 18.4 and 14.1, respectively. The results showed that curcumin and its new derivatives have remarkable antiviral effects on HSV-1 in cell culture.