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排序方式: 共有188条查询结果,搜索用时 250 毫秒
91.
Sophie Carbonnelle Joris Van Loco Ilse Van Overmeire Isabelle Windal Nathalie Van Wouwe Stefan Van Leeuwen Leo Goeyens 《Talanta》2004,63(5):753-1259
Differences between chemical activated luciferase gene expression (CALUX) bioassay and chemoanalyses results are observed.
This paper shows that calculations of the TEQ values using REP values instead of WHO TEF values give different results. The REP values do affect the results obtained by the CALUX technique. These differences are more marked for the dioxin like PCB compounds (CALUX TEQ values are lower than WHO TEQ values) than for the dioxin compounds (CALUX TEQ values are higher than WHO TEQ values).
The CALUX results were compared with the concentrations of the congeners’ spiked into the oil. 相似文献
92.
Ilse M. Welleman Mark W. H. Hoorens Ben L. Feringa Hendrikus H. Boersma Wiktor Szymaski 《Chemical science》2020,11(43):11672
Light-based therapeutic and imaging modalities, which emerge in clinical applications, rely on molecular tools, such as photocleavable protecting groups and photoswitches that respond to photonic stimulus and translate it into a biological effect. However, optimisation of their key parameters (activation wavelength, band separation, fatigue resistance and half-life) is necessary to enable application in the medical field. In this perspective, we describe the applications scenarios that can be envisioned in clinical practice and then we use those scenarios to explain the necessary properties that the photoresponsive tools used to control biological function should possess, highlighted by examples from medical imaging, drug delivery and photopharmacology. We then present how the (photo)chemical parameters are currently being optimized and an outlook is given on pharmacological aspects (toxicity, solubility, and stability) of light-responsive molecules. With these interdisciplinary insights, we aim to inspire the future directions for the development of photocontrolled tools that will empower clinical applications of light.This perspective article explores the current state of light-controlled molecular tools for medical therapy and imaging and offers an outlook on clinical application scenarios and optimisation strategies. 相似文献
93.
Leclercq L Delatour C Hoes I Brunelle F Labrique X Castro-Perez J 《Rapid communications in mass spectrometry : RCM》2005,19(12):1611-1618
Metabolism data provided with reduced cycle time has become of increasing importance for the early evaluation of DMPK properties of drugs in discovery. In this regard, quadrupole time-of-flight hybrid mass spectrometers (Q-TOF) can provide very reliable metabolite identification via accurate mass measurement of ions and the consequent access to the elemental composition of the metabolite. However, due to their cost, they are often used for drug metabolism studies on later stage drug candidates or to address challenging metabolism questions. A new prototype, consisting of a five-channel multiplexed electrospray ionization (ESI) source on a Q-TOF with one channel used for lock-mass compound infusion, was evaluated for metabolite identification. The goal was to increase the sample throughput of a single ESI-MS system by a factor of 4, while maintaining efficient metabolite separation in high-performance liquid chromatography (HPLC) as well as adequate sensitivity and mass accuracy, and ultimately improve the speed and quality of metabolism studies supporting drug discovery. The analytical performance of the system was assessed by evaluating the sensitivity and mass accuracy (using real in vitro and in vivo samples), inter-channel differences in retention times, MS/UV response, and cross-talk among channels. The sensitivity using the multiplexed ESI source was on average 2-fold lower than with single ESI, correlating well with previous literature data. The mass accuracy was comparable to that obtained using single ESI in both MS and MS/MS modes, making the metabolite identification process using the multiplexed ESI source as reliable as with single ESI. Compound-dependent differences in ionization efficiencies were observed among channels, and were minimized by analyzing related samples on the same channel. Finally, the level of cross-talk among channels was acceptable (around 0.3%) and comparable to levels previously published for quantitative applications using multiplexed ESI. The paper also focuses on the advantages and disadvantages of this new approach compared to other approaches in the literature in the field of metabolite identification. 相似文献
94.
Winfried Wendelin Wolfgang Kern Ilse Zmölnig Hans-Wolfgang Schramm 《Monatshefte für Chemie / Chemical Monthly》1981,112(8-9):1091-1098
The aminonitriles3a–f react with guanidine inDMF to yield the 5,5-resp. 5-subtituted imidazolidine-2,4-diimines2a–d resp.2f, whereas2e could not be isolated. 7,14-diazadispiro[5.1.5.2]-pentadecan-15-on (7)1, 4-imino-1,3-diazaspiro[4.5]decan-2-one (8a)1 and the 2,4-diaminotriazine (9)1 were isolated as byproducts.1-Aminocyclohexancarbonitrile (3a) reacts with urea to the 4-imino-1,3-diazaspiro[4.5]decan-2-one (8a)1;8a can be prepared from (1-cyancyclohexyl)urea (11a) as well. The structures of the new compounds are proved by NMR-, IR- and mass spectra and the mechanism of the reaction is discussed.
Herrn Prof. Dr.G. Zigeuner zum 60. Geburtstag gewidmet. 相似文献
95.
Iron uptake and toxicity in Caco-2 cells 总被引:2,自引:0,他引:2
Bettina Zdl Michaela Zeiner Patricia Paukovits Ilse Steffan Wolfgang Marktl Cem Ekmekcioglu 《Microchemical Journal》2005,79(1-2):393
The differences between the in vitro effects of iron attributed to valence, chelation, and complexation are known in terms of markers of oxidative stress. Few studies, however, describe the effects of iron on general markers of toxicity used in the testing of cell cultures. The aim of the present study was to determine the toxicity and uptake of different salts and iron complexes in the human intestinal cell line, Caco-2.Cells were incubated with 1.5 mM of different species of iron [FeCl3/nitrilotriacetic acid (NTA) (1:2), FeCl3/citric acid (1:2), FeCl3 and FeSO4] for 22–24 h. Thereafter, toxicological and uptake experiments were performed.The iron uptake, viability (via MTT assay), and membrane stability (via LDH release) of Caco-2 cells incubated with various iron forms differed significantly from untreated controls which showed no detrimental effects on cells and less iron uptake. The lowest signal for cell viability (MTT assay) was found after the incubation of the cells with FeCl3/citric acid, being significantly different to treatment with FeCl3, where the highest MTT signal was detected (p=0.002). No differences between the tested iron species could be found regarding cell proliferation (via serial cell counting) and viability using the trypan blue exclusion test. The lowest membrane damage (via LDH release) was registered in cells treated with FeCl3/citric acid (1:2), whereas the highest LDH release could be found in cells incubated with FeCl3/NTA (1:2). The highest intracellular iron concentration (measured via GFAAS) was detected after the treatment of Caco-2 cells with FeCl3 and FeCl3/NTA (1:2).This study substantiates the importance of the choice of complexes, as NTA seemed to enhance the toxicity of iron, while citric acid inhibited iron uptake and toxicity. 相似文献
96.
Dr. Francesco Manoli Dr. Filippo Doria Prof. Giorgio Colombo Dr. Barbara Zambelli Mauro Freccero Dr. Ilse Manet 《Chemistry (Weinheim an der Bergstrasse, Germany)》2021,27(45):11707-11720
Human telomeric DNA with hundreds of repeats of the 5’-TTAGGG-3’ motif plays a crucial role in several biological processes. It folds into G-quadruplex (G4) structures and features a pocket at the interface of two contiguous G4 blocks. Up to now no structural NMR and crystallographic data are available for ligands interacting with contiguous G4s. Naphthalene diimide monomers and dyads were investigated as ligands of a dimeric G4 of human telomeric DNA comparing the results with those of the model monomeric G4. Time-resolved fluorescence, circular dichroism, isothermal titration calorimetry and molecular modeling were used to elucidate binding features. Ligand fluorescence lifetime and induced circular dichroism unveiled occupancy of the binding site at the interface. Thermodynamic parameters confirmed the hypothesis as they remarkably change for the dyad complexes of the monomeric and dimeric telomeric G4. The bi-functional ligand structure of the dyads is a fundamental requisite for binding at the G4 interface as only the dyads engage in complexes with 1 : 1 stoichiometry, lodging in the pocket at the interface and establishing multiple interactions with the DNA skeleton. In the absence of NMR and crystallographic data, our study affords important proofs of binding at the interface pocket and clues on the role played by the ligand structure. 相似文献
97.
98.
Margot Goehring und Ilse Darge 《Fresenius' Journal of Analytical Chemistry》1943,125(5-6):180-184
Ohne Zusammenfassung 相似文献
99.
Lipovšek S Letofsky-Papst I Hofer F Leitinger G Devetak D 《Micron (Oxford, England : 1993)》2012,43(5):651-665
We analysed structural differences between midgut epithelial cells of fed instar antlions' larvae Euroleon nostras and starved ones. In starved larvae the presence of autophagolysosome-like structures was observed, which are characteristic structures associated with autophagy. The results presented here provide insight supporting the role of autophagy as a cell survival mechanism for the periods of food deprivation. Additional structural changes in the cytoplasm were seen in the spherites. The ultrastructure and chemical composition of spherites in the midgut epithelial cells of first, second and third instar larvae were observed with light microscopy and transmission electron microscopy (TEM). A detailed characterization of the elemental composition of the spherites was studied using analytical electron microscopy; a combination of energy dispersive X-ray spectroscopy (EDXS), electron energy-loss spectroscopy (EELS) and energy filtering TEM (EFTEM) was applied. The structure and elemental composition of the spherites changed during the period of larval life. Moreover, changes in chemical composition were found between spherites from fed and starved E. nostras. In fed first instar larvae, the spherites contained an organic matrix, composed of C, N and O. In this matrix, P, Cl, Ca and Fe were detected. In starved first instar larvae, only C, N and P were present. The spherites of fed second instar larvae were rich in organic and inorganic elements and were composed of C, N, O, Na, Mg, P, S, Cl, K, Ca, Mn, Fe and Zn. In starved second instar larvae, the chemical elements N, O, P, Ca and Fe were found. In fed third instar larvae, the spherites contained C, N, O, Na, Mg, P, Cl, K, Ca, Mn, Fe, Co and Zn. In starved third larvae, C, O, Si, Ca, and Fe were detected. Generally, the spherites are exploited in starved larvae. These results suggest that the elemental supply of spherites may provide crucial support for physiological processes during starvation periods amongst E. nostras instar larvae. In some cases in fed second and fed third instar larvae, spherites were seen in the lumen of the midgut. Such spherites could serve as reservoirs for nontoxic waste material that cannot be metabolized. 相似文献
100.
Experimental Techniques - It has been shown that with the same load fringe patterns of the Flamant and Boussinesq problems are similar. However, Figs. 2 and 3 show that if the load is concentrated... 相似文献