Determination of Testosterone Metabolites in Rat Hepatocytes with and without Cryopreservation by On-Line SPE Column-Switching LC and MS Detection

In a recently published paper development of a sensitive automated “on-line” solid-phase extraction (SPE)/RP-HPLC assay for 6β-hydroxytestosterone (6β-OHT) with corticosterone as the internal standard (IS) was reported and its potential for quantification of various testosterone metabolites in culture media reflecting metabolic activity of cultured human and animal hepatocytes demonstrated [1]. In this following contribution the technique has been extended to determination of another five testosterone metabolites in cultured rat hepatocytes using an identical “on-line” SPE/RP-HPLC procedure and detection by tandem MS-MS with an atmospheric pressure chemical ionization (APCI) source in the selected reaction monitoring (SRM) mode as that described in [1]. All six testosterone metabolites, namely 2α-OHT, 2β-OHT, 6α-OHT, 6β-OHT, 7α-OHT and 16α-OHT, could be sufficiently separated from each other and thus an unequivocal assignment to the individual structures was achieved. Validation data are presented specifying the limits of quantitation as well as the mean values of the coefficients of variation (CV) for the target analytes and the accuracy obtained at five different days. Regio- and stereoselective testosterone hydroxylation by rat hepatocytes was measured in a long-term culture system with and without exposure to rifampicin as an inducer of liver CYP 3A4 activity. In addition, testosterone hydroxylation was analyzed in cultures of cryopreserved hepatocytes that had been stored at −196 °C. The rat hepatocytes were cultured after thawing for up to 11 days and induction of testosterone hydroxylase activity could be demonstrated in cultures which underwent a new cryopreservation protocol. This paper is dedicated to Professor Hinrich Cramer on the occasion of his 75th birthday.     

Can shadowing mimic the QCD phase transition?

The directed flow of protons is studied in the quark-gluon string model as a function of the impact parameter for S+S and Pb+Pb reactions at 160 AGeV/c. A significant reduction of the directed flow in midrapidity range, which can lead to the development of the antiflow, is found due to the absorption of early emitted particles by massive spectators (shadowing effect). This effect can mimic the formation of the quark-gluon plasma (QGP). However, in the absorption scenario the antiflow is stronger for the system of light colliding nuclei than for the heavy ones, while in the case of the plasma creation the effect should be opposite.     

Ueber lineare homogene Differentialgleichungen,zwischen deren Integralen homogene Relationen höheren als ersten Grades bestehen

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Structural investigation of icosahedral Pd58.8U20.6Si20.6

The structure factors of the amorphous, icosahedral and crystalline Pd_58.8U_20.6Si_20.6 were measured with X-rays and neutrons. By comparing the radial distribution functions of the three phases, we find a similar short range order in the amorphous and icosahedral phase, but a different atomic arrangement in the crystalline phase. A magnetic long range order observed in the crystalline material is absent in the icosahedral phase.Dedicated to Professor Harry Thomas on the occasion of his 60th birthday     

On quantitative aspects of the unit sum number problem

We investigate the function u _K,S (n; q) which counts the number of representations of algebraic integers α with |N_{K/\mathbb Q}(a)| ￡ q{|N_{K/{\mathbb Q}}(\alpha)| \leq q} for some real positive q that can be written as sums of exactly n S-units of the number field K.     

Untersuchung von Mehl

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Jodzahl

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