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41.
42.
A novel totally screen-printed flow-through cell for immunoanalysis is presented. It contained screen-printed carbonaceous electrodes, which allowed the determination of peroxidase activity through the electrochemical reduction of p-benzoquinone. As different electrode materials differ strongly in their electrochemical properties, electrodes resulting from various screen-printable carbonaceous pastes were characterized using the hydroquinone/ p-benzoquinone redox couple. For most of the electrodes, cyclic voltammogram peak separations of between 550 and 670 mV were observed indicating only quasi-reversible electrochemical behavior. This was confirmed by variation of the peak separation with scan rate. Heterogeneous electron transfer rates of ca. 0.5 - 1 x 10(-3) cm s(-1) and electrochemical activation energies of ca. 20 kJ mol(-1) were found. These flow-through cells were not only applied to electrochemical peroxidase activity determinations but also, in combination with a separate detector, as affinity reactors. After biotinylation of screen-printed layers, streptavidin and then biotinylated peroxidase could be bound. However, as signals were only 10-20% of those obtained with a column filled with biotinylated glass beads, only the screen-printed electrochemical detector was applied to the detection of antibodies against the African Swine Fever Virus. 相似文献
43.
Summary The selective binding of 3-indole acetic acid (IAA) alkali phosphatase (AP) to respective antibodies immobilized on pretreated titanium wires as electrodes (40×0.8 mm ø) is studied and the conditions are optimized with respect to selectivity and sensitivity, and the cross-reactivity of some related compounds is determined. Using these experiences, such wires are used as electrodes to detect selectively the methylester of IAA (IAA-Me). They are shown to behave best when the change of the electrode potential is measured against a similar reference electrode, coated with immobilized immuno globulin G (IgG) of the respective pre-immuno serum. A second coating with bovine serum albumin (BSA) is shown to prevent unspecific binding of IAA-Me. The sensitivity goes down to around 100 pmol/ml.
Part I: Fresenius J Anal Chem (1992) 343:541 相似文献
44.
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46.
A new procedure for the synthesis of 2,3-diaryl-3,4-dihydro-4-hydroxy-1 (2
)-isoquinolones is described in which the
-isomer predominates. Dehydration leads to 2,4-diarylisocarbostyrils. 相似文献
47.
Ursula Baumann 《Fresenius' Journal of Analytical Chemistry》1961,183(3):207
Ohne Zusammenfassung 相似文献
48.
49.
Ursula Baumann 《Fresenius' Journal of Analytical Chemistry》1963,193(6):463
Ohne Zusammenfassung
Abderhalden, E.: Abwehrfermente. Verlag Th. Steinkopff, Dresden, 7. Auflage 1944. 相似文献
50.
Ursula Baumann 《Fresenius' Journal of Analytical Chemistry》1964,206(6):455-456
Ohne Zusammenfassung 相似文献