Surface-supported bilayers with transmembrane proteins: role of the polymer cushion revisited

Protein lateral mobility in surface-supported bilayers is often much lower than the mobility of the lipids. In the present study we explore whether the incorporation of a PEG cushion between the bilayer and the substrate increases the lateral mobility of transmembrane proteins in bilayers produced via directed assembly, a method based on Langmuir-Blodgett deposition techniques. In our experiments, the PEG cushions were incorporated by adding PEG lipids to the protein/lipid monolayer at the air/water interface, at the first step of bilayer assembly. The protein and lipid mobilities in 160 different bilayers, with various PEG molecular weights and PEG lipid concentrations, were measured and compared. We found that the measured diffusion coefficients do not depend on the PEG molecular weight or the PEG lipid concentration and are very similar to the values measured in the absence of PEG. Therefore, contrary to our expectations, we found that a PEG cushion does not necessarily increase protein mobility, suggesting that the low protein mobility is not a consequence of protein-substrate interactions. Furthermore, we showed that the low protein mobility is not due to protein aggregation. The major determinant of protein mobility in surface-supported bilayer systems appears to be the method of bilayer assembly. While proteins were always mobile if the bilayers were prepared using the directed assembly method, in the presence and absence of a PEG cushion, other bilayer assembly protocols resulted in complete lack of protein mobility.     

Computer-generated true-color rainbow holograms

A new method of calculating computer-generated true-color rainbow holograms (CGTCRH) is proposed. This technique allows the true-color object reconstruction. The main idea of the presented work is based on the additive properties of holographic wavefront reconstruction such that the resulting hologram is a superposition of three (or more) holograms, with each of them producing different color reconstruction of the same object. White light and monochromatic light reconstruction results are discussed.     

正硅酸锌中能量传递的激子机理

在α型正硅酸锌中,用接近吸收边(Eg～5.5eV)的光能激发,发现了激子能量传递到发光中心的某些特征。在一个弛豫状态上,激子扩散的激活能估计为0.075eV,激子在自局域化前通过的扩散体积为0.5×10-19cm-3。实验确定的磷光体中能量传递的参数可实用于选择最佳颗粒尺寸分布。     

Thermoelastoplastic Nonaxisymmetric Stress-Strain Analysis of Laminated Shells of Revolution

A technique for nonaxisymmetric thermoelastoplastic stress–strain analysis of laminated shells of revolution is developed. It is assumed that there is no slippage and the layers are not separated. The problem is solved using the geometrically linear theory of shells based on the Kirchhoff–Love hypotheses. The thermoplastic relations are written down in the form of the method of elastic solutions. The order of the system of partial differential equations obtained is reduced by means of trigonometric series in the circumferential coordinate. The systems of ordinary differential equations thus obtained are solved by Godunov's discrete-orthogonalization method. The nonaxisymmetric thermoelastoplastic stress–strain state of a two-layered shell is analyzed as an example     

Thermoelastoplastic deformation of noncircular cylindrical shells

A method to determine the nonstationary temperature fields and the thermoelastoplastic stress-strain state of noncircular cylindrical shells is developed. It is assumed that the physical and mechanical properties are dependent on temperature. The heat-conduction problem is solved using an explicit difference scheme. The temperature variation throughout the thickness is described by a power polynomial. For the other two coordinates, finite differences are used. The thermoplastic problem is solved using the geometrically nonlinear theory of shells based on the Kirchhoff-Love hypotheses. The theory of simple processes with deformation history taken into account is used. Its equations are linearized by a modified method of elastic solutions. The governing system of partial differential equations is derived. Variables are separated in the case where the curvilinear edges are hinged. The partial case where the stress-strain state does not change along the generatrix is examined. The systems of ordinary differential equations obtained in all these cases are solved using Godunov's discrete orthogonalization. The temperature field in a shell with elliptical cross-section is studied. The stress-strain state found by numerical integration along the generatrix is compared with that obtained using trigonometric Fourier series. The effect of a Winkler foundation on the stress-strain state is analyzed Translated from Prikladnaya Mekhanika, Vol. 44, No. 8, pp. 79–90, August 2008.