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排序方式: 共有279条查询结果,搜索用时 718 毫秒
271.
Sotgia S Carru C Franconi F Fiori PB Manca S Pettinato S Magliona S Ginanneschi R Deiana L Zinellu A 《Journal of chromatography. A》2008,1185(1):145-150
We propose a new capillary zone electrophoresis method applying short-end injection technique for the fast evaluation of methylcystosine/total cytosine ratio after acidic DNA hydrolysis. By short-end injection and by using a 100 mmol/l Tris solution titrated with 1 mol/l phosphoric acid to pH 3.75 as background electrolyte, cytosine and methylcytosine were separated with a good resolution in less than 1.5 min. Stepwise multiple linear regression with DNA methylation degree as the dependent variable and age, cysteine, homocysteine and methionine as independent variables, showed a negative association with age and that total cysteine is the most important determinant of DNA methylation. 相似文献
272.
Eugenio Aprea Franco Biasioli Silvia Carlin Tilmann D. Mrk Flavia Gasperi 《International journal of mass spectrometry》2008,275(1-3):117-121
The presence of benzene in food and in particular in soft drinks has been reported in several studies and should be considered in fundamental investigations about formation of this carcinogen compound as well as in quality control.Proton transfer reaction-mass spectrometry (PTR-MS) has been used here for rapid, direct quantification of benzene and to monitor its formation in model systems related to the use of benzoate, a common preservative, in presence of ascorbic acid: a widespread situation that yields benzene in, e.g., soft drinks and fruit juices.Firstly, we demonstrate here that PTR-MS allows a rapid determination of benzene that is in quantitative agreement with independent solid phase micro-extraction/gas chromatography (SPME/GC) analysis. Secondly, as a case study, the effect of different sugars (sucrose, fructose and glucose) on benzene formation is investigated indicating that they inhibit its formation and that this effect is enhanced for reducing sugars. The sugar-induced inhibition of benzene formation depends on several parameters (type and concentration of sugar, temperature, time) but can be more than 80% in situations that can be expected in the storage of commercial soft drinks. This is consistent with the reported observations of higher benzene concentrations in sugar-free soft drinks. 相似文献
273.
Francesca Baldassarre Viviana Vergaro Flavia Scarlino Flavia De Santis Giovanna Lucarelli Antonio della Torre Giuseppe Ciccarella Ross Rinaldi Gianluigi Giannelli Stefano Leporatti 《Macromolecular bioscience》2012,12(5):656-665
The efficient internalization of TGF‐beta inhibitor‐loaded polyelectrolyte capsules and particles is studied in two HCC cell lines. Two polyelectrolyte pairs (biocompatible but not degradable and biodegradable crosslinked with gluteraldehyde) are employed for coating. The capsules are characterized by SEM. LY is successfully loaded inside the core and embedded between polymer layers. MS is used to quantify the loading efficiency by comparing post‐loading and core‐loading methods, since both coated templates and hollow shells are used as carriers. CLSM confirms dissolution of the pre‐formed multilayer upon enzymatic degradation as the method of release, and migration assays demonstrate a higher inhibition efficiency of TGF‐beta in tailored biodegradable capsules compared to free LY administration.
274.
Flavia Popa Pedro Lameiras Oana Moldovan Maria Tomoaia-Cotisel Eric Henon Agathe Martinez Carmen Sacalis Aurora Mocanu Yvan Ramondenc Mircea Darabantu 《Tetrahedron》2012,68(43):8945-8967
The iterative chemoselective amination of cyanuric chloride to dimers of new G-2 dendritic N-substituted-2,4,6-triamino-s-triazines (melamines) having C-2-substituted 2-aminopropane-1,3-diols (‘serinols’) in tandem with the ethylene ketal of 4-piperidone as peripheral units is reported. The structure as a function of increasing molecular size was studied by NMR spectroscopy, DFT calculation and AFM imaging. A concise nomenclature defining the restricted rotational phenomena about the newly created C(s-triazine)–N(exocyclic) partial double bonds, seen as axes of (pro)diastereomerism, is used. We propose a new form of frontier rotamerism for the dendrimer surface, which operates over a long range. 相似文献
275.
276.
Flavia Jankowski 《Tetrahedron》2010,66(1):128-133
A general method has been developed for the synthesis of 1,3-disubstituted-imidazo[1,5-a]quinazolin-5-(4H)-ones. This process involves initial microwave-assisted quinazolinone formation between anthranilamide and various Boc- or acylamino acids, followed by intramolecular cyclodehydration under acidic conditions. In the case of 3-monosubstituted-imidazoquinazolinones, the procedure needs the formation of the formamide derivatives by deprotection and formylation of the Boc-intermediates. 相似文献
277.
A clique-transversal of a graph G is a subset of vertices that meets all the cliques of G. A clique-independent set is a collection of pairwise vertex-disjoint cliques. A graph G is clique-perfect if the sizes of a minimum clique-transversal and a maximum clique-independent set are equal for every induced subgraph of G. The list of minimal forbidden induced subgraphs for the class of clique-perfect graphs is not known. Another open question concerning clique-perfect graphs is the complexity of the recognition problem. Recently we were able to characterize clique-perfect graphs by a restricted list of forbidden induced subgraphs when the graph belongs to two different subclasses of claw-free graphs. These characterizations lead to polynomial time recognition of clique-perfect graphs in these classes of graphs. In this paper we solve the characterization problem in two new classes of graphs: diamond-free and Helly circular-arc () graphs. This last characterization leads to a polynomial time recognition algorithm for clique-perfect graphs. 相似文献
278.
Dr. Fabio Pirro Dr. Salvatore La Gatta Dr. Federica Arrigoni Dr. Antonino Famulari Dr. Ornella Maglio Prof. Pompea Del Vecchio Prof. Mario Chiesa Prof. Luca De Gioia Prof. Luca Bertini Dr. Marco Chino Prof. Flavia Nastri Prof. Angela Lombardi 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2023,135(1):e202211552
De novo metalloprotein design is a remarkable approach to shape protein scaffolds toward specific functions. Here, we report the design and characterization of Due Rame 1 (DR1), a de novo designed protein housing a di-copper site and mimicking the Type 3 (T3) copper-containing polyphenol oxidases (PPOs). To achieve this goal, we hierarchically designed the first and the second di-metal coordination spheres to engineer the di-copper site into a simple four-helix bundle scaffold. Spectroscopic, thermodynamic, and functional characterization revealed that DR1 recapitulates the T3 copper site, supporting different copper redox states, and being active in the O2-dependent oxidation of catechols to o-quinones. Careful design of the residues lining the substrate access site endows DR1 with substrate recognition, as revealed by Hammet analysis and computational studies on substituted catechols. This study represents a premier example in the construction of a functional T3 copper site into a designed four-helix bundle protein. 相似文献
279.