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961.
Ricerche di Matematica - We prove that some of the main results of linkage theory can be extended to a more general context in homological algebra. Our main result states that, under suitable... 相似文献
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Funda Alkan Hanife Sevgi Varlı Ph.D. Murat Demirbilek Ph.D. Engin Kaplan Ph.D. Nelisa Türkoğlu Laçin 《ChemistryOpen》2020,9(11):1181-1189
Induced pluripotent stem cells (IPSC) are preferred as an alternative source for regenerative medicine, disease modeling, and drug screening due to their unique properties. As seen from the previous studies in the literature, most of the vector systems to transfer reprogramming factors are viral-based and have some well-known limitations. This study aims to develop a non-viral vector system for the transfection of reprogramming factors. Cationic stearamide lipid nanoparticles (CSLN) were prepared via the solvent diffusion method. The obtained CSLNs were used for the delivery of plasmid DNA (pDNA) encoding Oct3/4, Sox2, Klf4, and GFP to fibroblast cell lines. The optimization studies, for zeta potential and particle size of the conjugate, was performed to achieve high cell viability. CSLN63 with 36.5±0.06 mV zeta potential and 173.6±13.91 nm size was used for the transfection of Fibroblast cells. The transfection efficiency was observed by following GFP expression and was found as 70 %±0.11. The expression of the Oct4, Sox2, Klf4 was determined by RT-qPCR; an increase was observed after the 12th cycle in Klf4 (Ct averages: 13,41), Sox2 (Ct averages; 12,4), Oct4 (Ct average; 13,77). The tendency of colonization was observed. The upregulation efficiency of Oct4 and SSEA-1 with CSLN and another non-viral vector designed for the transportation of Yamanaka factors developed in our lab previously were compared with flow cytometer analysis. 相似文献
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Lara Jabbour Roberta Bongiovanni Didier Chaussy Claudio Gerbaldi Davide Beneventi 《Cellulose (London, England)》2013,20(4):1523-1545
Li-ion batteries (LIBs) are the most employed power source in portable electronics (e.g., cellular phones, laptop computers…) and are accounted as very promising storage/power systems for future electric/hybrid-electric powered transportation. However for their future development, low production costs and environmental friendliness will be key parameters. In this context, the introduction of water processable biosourced polymers such as cellulose and its derivatives is very interesting and is emerging as a viable route toward the development of green materials and processes for LIB manufacturing. The present review briefly introduces the Li-ion technology and gives an overview on cellulose and cellulose derivatives for the elaboration of separators, electrolytes and electrodes. 相似文献
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Ternary Hybrid γ‐Fe2O3/CrVI/Amine Oxidase Nanostructure for Electrochemical Sensing: Application for Polyamine Detection in Tumor Tissue 下载免费PDF全文
Dr. Emanuela Bonaiuto Dr. Massimiliano Magro Dr. Davide Baratella Dr. Petr Jakubec Elisabetta Sconcerle Dr. Milo Terzo Dr. Giovanni Miotto Dr. Alberto Macone Prof. Enzo Agostinelli Dr. Silvano Fasolato Dr. Rina Venerando Prof. Gabriella Salviulo Dr. Ondrej Malina Prof. Radek Zboril Prof. Dr. Fabio Vianello 《Chemistry (Weinheim an der Bergstrasse, Germany)》2016,22(20):6846-6852
Dichromate binds to surface‐active maghemite nanoparticles (SAMNs) to form a stable core–shell nanostructures (SAMN@CrVI). The hybrid was characterized by Mössbauer spectroscopy, high‐angle annular dark‐field imaging, electron energy‐loss spectroscopy, and electrochemical techniques, which revealed a strong interaction of dichromate with the nanoparticle surface. Electrochemical characterization showed lower charge‐transfer resistance, better electrochemical performance, and more reversible electrochemical behavior with respect to naked SAMNs. Moreover, SAMN@CrVI is an excellent electrocatalyst for hydrogen peroxide reduction. Furthermore, an enzyme, namely, bovine serum amine oxidase (BSAO: EC 1.4.3.6), was immobilized on SAMN@CrVI by self‐assembly to give a ternary hybrid nanostructured catalyst for polyamine oxidation (SAMN@CrVI‐BSAO). SAMN@CrVI‐BSAO was applied for the development of a reagentless, fast, inexpensive, and interference‐free polyamine biosensor, which was successfully exploited for the discrimination of tumorous tissue from healthy tissue in human crude liver extracts. 相似文献
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