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41.
Bird D  Gu M 《Optics letters》2002,27(12):1031-1033
We present a two-photon fluorescence microscope based on a three-port single-mode optical fiber coupler. It is found that the coupler behaves as a low-pass filter that can deliver an ultrashort-pulsed laser beam of as much as 150 mW of power in the wavelength range from 770 to 870 nm as well as collect a two-photon fluorescence signal in the visible range. As a result of using the fiber coupler, the new two-photon imaging system exhibts a number of advantages, including a compact arrangement, freedom from vibration from lasers and electronic devices, self-alignment, reduction of multiple scattering, and an enhanced optical sectioning effect. The effectiveness of the new instrument is demonstrated with a set of three-dimensional images of biological samples. This instrument may make two-photon fluorescence endoscopy possible for in vivo medical applications.  相似文献   
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A theorem of Green, Lazarsfeld and Simpson (formerly a conjecture of Beauville and Catanese) states that certain naturally defined subvarieties of the Picard variety of a smooth projective complex variety are unions of translates of abelian subvarieties by torsion points. Their proof uses analytic methods. We refine and give a completely new proof of their result. Our proof combines galois-theoretic methods and algebraic geometry in positive characteristic. When the variety has a model over a function field and its Picard variety has no isotrivial factors, we show how to replace the galois-theoretic results we need by results from model theory (mathematical logic). Furthermore, we prove partial analogs of the conjecture of Beauville and Catanese in positive characteristic.  相似文献   
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The inelastic neutron scattering (INS) and periodic density functional theory (DFT) vibrational spectra of H3B:NH3 are reported to 1600 cm-1. The H3B:NH3 structural and INS features, specifically the reduced solid-state B:N dative bond length and the altered B:N stretching frequency, are reproduced by the periodic DFT calculations, placing the B:N stretching mode at 800 cm-1, in excellent agreement with experiment relative to previous nonperiodic theoretical treatments of this molecule.  相似文献   
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Herein, it is reported that the polymorphism in the helical nanofilament (HNF, B4) liquid-crystalline phase depends on the fabrication methods, that is, UV-driven formation and template-assisted self-assembly in the nanoconfined geometry. As a result, uniaxially oriented HNFs with different helical structures were obtained, in which generation of the twisted-ribbon and cylindrical-ribbon polymorphs showed that even the molecular lattice has a different orientation. The detailed structures were directly observed by SEM and grazing-incidence X-ray diffraction with synchrotron radiation. The resultant polymorphs could be used in chiro-optical applications due to the capability for fine control of the helical structures.  相似文献   
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