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141.
Swetha Mahalaxmi Colin R. Jackson Clint Williford Charles L. Burandt 《Applied biochemistry and biotechnology》2010,162(5):1414-1422
Biochemical conversion of lignocellulosic biomass to ethanol involves size reduction, preprocessing, pretreatment, enzyme
hydrolysis, and fermentation. In recent years, microbial preprocessing has been gaining attention as a means to produce labile
biomass for lessening the requirement of pretreatment severity. However, loss of sugars due to microbial consumption is a
major consequence, suggesting its minimization through optimization of nutrients, temperature, and preprocessing time. In
this work, we emphasized estimation of fungal preprocessing time, at which higher sugar yields can be achieved after preprocessing
and enzyme hydrolysis. The estimation is based on the enzymatic activity profile obtained by treating switchgrass with Phanerochaete chrysosporium for 28 days. Enzyme assays were conducted once in every 7 days for 28 days, for activities of phenol oxidase, peroxidase,
β-glucosidase, β-xylosidase, and cellobiohydrolase. We found no activity for phenol oxidase and peroxidase, but the greatest
activities for cellulases on the seventh day. We then treated switchgrass for 7 days with P. chrysosporium and observed that the preprocessed switchgrass had higher glucan (39%), xylan (17.5%), and total sugar yields (25.5%) than
the unpreprocessed switchgrass (34%, 37.5%, and 20.5%, respectively, p < 0.05). This verifies the utility of using enzyme assays for initial estimation of preprocessing time to enhance sugar yields. 相似文献
142.
Julien Labaune J. Bianca Jackson Kaori Fukunaga Jeffrey White Laura d’Alessandro Alison Whyte Michel Menu Gerard Mourou 《Applied Physics A: Materials Science & Processing》2011,105(1):5-9
Terahertz Time Domain Imaging has been used in the last few years for the investigation of cultural heritage. In this article,
the authors demonstrate the possibility to apply it for the investigation of clay artifacts. Tomographic images were obtained
of a model in reflection, and an Egyptian vessel in transmission. 相似文献
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145.
Streptothricin F (STF, 1) is a peptidyl nucleoside antibiotic produced by Streptomyces lavendulae. Studies were conducted to address the formation and timing of incorporation of the arginine-derived base streptolidine (4) during the biosynthesis of 1. [guanidino-(13)C]Streptolidine (10) was prepared by modification of an established method and used in whole-cell incorporation experiments. Analysis of the purified STF by (13)C NMR revealed a 1.9% enrichment of the guanidino carbon, confirming 4 as an advanced precursor to 1 and supporting proposals that 1 is assembled via a convergent biosynthetic pathway. To identify advanced intermediates in the conversion of L-arginine to 4, (2S,3R)-[guanidino-(13)C]capreomycidine (32) was prepared from oxazolidine aldehyde (18) via 1,1-dimethylethyl (4R,1'S)-4-(1',3'-diaminopropyl)-2,2-dimethyl-3-oxazolidinecarboxylate (30). Treatment of 30 with Br(13)CN yielded the corresponding diprotected amino alcohol, which was readily converted to 32. The STF isolated from whole-cell incorporation experiments with 32 showed no significant (13)C enrichment at the guanidino carbon. These results suggest that 32 may be an enzyme-bound intermediate, unable to enter the cell, or is not a precursor to STF. 相似文献
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147.
Jackson BP Shaw Allen PL Hopkins WA Bertsch PM 《Analytical and bioanalytical chemistry》2002,374(2):203-211
Analytical techniques used to examine the chemical speciation of multiple trace elements are important for the investigation of biological systems. Size exclusion chromatography (SEC) coupled to ICP-MS was used to investigate the speciation of Se, As, Cu, Cd and Zn in tissue extracts from a largemouth bass (Micropterus salmoides) collected from a coal fly ash basin and results were compared to a largemouth bass collected at a reference site. Using a Biosil SEC column, with an effective separation range of 100-7 KDa, Cu, Zn and Cd were shown to be bound to metallothionein (MT) in the liver, gill and, to a lesser extent, gonad tissue extract. In liver, muscle and gill of the ash basin bass, Se was predominantly present as low molecular weight species. Only in the gonad extract was the major fraction of Se associated with high molecular weight species. For the liver and gill extracts, further SEC-ICP-MS on a column with an effective separation range of 7000-500 Da was performed, but Se species still eluted near the total volume of the column suggesting a low molecular weight organic or inorganic species. Ion chromatography (IC)-ICP-MS using an AS7 column and HNO(3) gradient elution indicated that the Se and As species in the liver and gill extracts had similar retention times but these retention times did not correspond to retention times for As(III), As(V), dimethylarsenate, arsenobetaine, Se(IV), Se(VI), seleno-methionine, or seleno-cystine. 相似文献
148.
We calculate the dispersion relation associated with a solitary wave in a quasi-one-dimensional Bose-Einstein condensate of atoms confined in a harmonic, cylindrical trap in the limit of weak and strong interactions. In both cases, the dispersion relation is linear for long-wavelength excitations and terminates at the point where the group velocity vanishes. We also calculate the dispersion relation of sound waves in both limits of weak and strong coupling. 相似文献
149.
Finite-temperature simulations are used to study quadrupole excitations of a trapped Bose-Einstein condensate. We focus specifically on the m = 0 mode, where a long-standing theoretical problem is to account for an anomalous variation of the mode frequency with temperature. We explain this behavior in terms of the excitation of two separate modes, corresponding to the coupled motion of the condensate and the thermal cloud. The relative amplitude of the modes depends sensitively on the temperature and on the frequency of the harmonic drive used to excite them. Good agreement with experiment is found for appropriate drive frequencies. 相似文献
150.
Density Functional Theory (DFT) has been applied to characterize the early stages of the reaction of dioxygenation of [(triphos)M(catecholate)]+ complexes [M = Co(III), Ir(III); triphos = MeC(CH2PPh2)3], which have been considered to be models of ring-opening dioxygenases. The structural features of the starting complexes and of the intermediate complexes formed by addition of O2 to the coordinated catecholato ion are well reproduced. The calculations showed that this preliminary stage can be obtained only when the oxygen molecule attacks the molecule on the catecholato site. 相似文献