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391.
Sidsel Ø. Andreasen Siow‐Feng Chong Mille B. L. Kryger Bettina E. B. Jensen Almar Postma Marie‐Helene Alves Brigitte Städler Kenneth N. Goldie Alexander N. Zelikin 《Particle & Particle Systems Characterization》2013,30(6):514-522
The assembly, cellular internalization, and cytotoxicity of nanoparticles based on physical hydrogels of poly(vinyl alcohol) (PVA) are reported. PVA nanoparticles are assembled using a liposomal templating technique followed by removal of the lipids using isopropanol, a process that requires the presence of a custom‐made block copolymer, poly(vinyl alcohol‐b‐vinyl pyrrolidone), to avoid aggregation of the nanoparticles. Polymer hydrogelation is induced via incubation in aqueous isopropyl alcohol solution, which results in PVA hydrogel nanoparticles (PVA HNP) with excellent colloidal stability and stability towards disintegration over at least 24 h. Pristine PVA HNP are found to be remarkably stealth‐like and exhibit negligible cellular internalization. This feature is likely inherent with the low fouling nature of PVA and makes PVA HNP attractive for targeted drug delivery with a low level of association with non‐targeted cells and tissues. Blending PVA with varied amounts of collagen results in colloidal hydrogel particles with a well pronounced tendency towards association with mammalian cells, specifically hepatocytes and endothelial cells. The association of PVA HNP elicits minimal changes in cellular proliferation, making these novel hydrogel particles convenient tools for drug delivery applications and creation of implantable artificial organelles and sensors. 相似文献
392.
Seiwert B Henneken H Karst U 《Journal of the American Society for Mass Spectrometry》2004,15(12):1727-1736
Ferrocenoyl piperazide is introduced as a new pre-column derivatizing agent for the analysis of various isocyanates in air samples using reversed-phase liquid chromatographic separation, electrochemical oxidation/ionization, and mass spectrometry. The nonpolar derivatives can be separated well using a phenyl-modified stationary phase and a formic acid/ammonium formate buffer of pH 3, which yields excellent separations, especially for one problematic group of isocyanates consisting of 2,4- and 2,6-toluylenediisocyanate (2,4- and 2,6-TDI) and hexamethylenediisocyanate (HDI). Electrochemical oxidation at low potentials (0.5 V versus Pd/H(2)) leads to formation of charged products, which are nebulized in a commercial atmospheric pressure chemical ionization (APCI) source, with the corona discharge operated only at low voltage. Limits of detection between 6 and 20 nmol/L are obtained for the isocyanate derivatives, and calibration is linear over at least two decades of concentration. The method is applied for the analysis of air after thermal degradation of a polyurethane foam, and it is demonstrated that it is suitable as well for the analysis of carboxylic acid chlorides and of isothiocyanates. 相似文献
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Mathematical Methods of Operations Research - 相似文献
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Mimi Gao David Gnutt Axel Orban Dr. Bettina Appel Francesco Righetti Prof. Dr. Roland Winter Prof. Dr. Franz Narberhaus Prof. Dr. Sabine Müller Prof. Dr. Simon Ebbinghaus 《Angewandte Chemie (International ed. in English)》2016,55(9):3224-3228
Precise secondary and tertiary structure formation is critically important for the cellular functionality of ribonucleic acids (RNAs). RNA folding studies were mainly conducted in vitro, without the possibility of validating these experiments inside cells. Here, we directly resolve the folding stability of a hairpin‐structured RNA inside live mammalian cells. We find that the stability inside the cell is comparable to that in dilute physiological buffer. On the contrary, the addition of in vitro artificial crowding agents, with the exception of high‐molecular‐weight PEG, leads to a destabilization of the hairpin structure through surface interactions and reduction in water activity. We further show that RNA stability is highly variable within cell populations as well as within subcellular regions of the cytosol and nucleus. We conclude that inside cells the RNA is subject to (localized) stabilizing and destabilizing effects that lead to an on average only marginal modulation compared to diluted buffer. 相似文献
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Bettina Dahl 《Mathematical Thinking and Learning》2013,15(3):305-312
This review of the monograph, International Perspectives on Social Justice in Mathematics Education, is not a chapter-by-chapter summary of each of the 14 chapters per se, but rather, revolves around three overarching themes. 相似文献
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Bettina Schweizer Hanswilly Müller Wolfgang Böhme 《Fresenius' Journal of Analytical Chemistry》1992,343(2):183-187
Summary The determination of ethanol with alcohol dehydrogenase is described as an example of enzymatic determination with the flow injection analysis system (FIAS). Both, sample and reagent, are successively injected into the carrier stream by using only one valve. Compared with other techniques, the principle described is more economical with regard to reagent consumption and analysis time. Basic experiments about this kind of reagent addition (dispersion, reproducibility, possibility for gradient dilution) were made by simulation with dye solution. The determination of ethanol is carried out using the stopped flow technique. The peristaltic pumps are stopped when the reaction zone is located in the flow cell, and the change of absorbance with time is monitored. Thus background signals and other matrix influences can be minimized. The method is tested under real conditions for the determination of alcohol in several beverages. 相似文献