Chymotrypsin inhibitory constituents from Haloxylon recurvum

Haloxylase, the triglyceride (1), unsaturated fatty acid (2), and saturated fatty acid methyl ester (3) have been isolated from the chloroform-soluble fraction of Haloxylon recurvum. Their structures have been elucidated through spectroscopic studies and chemical transformation. All these compounds showed significant inhibitory activity against the enzyme chymotrypsin in a concentration dependent fashion.     

Probing a complex of cytochrome c and cardiolipin by magnetic circular dichroism spectroscopy: implications for the initial events in apoptosis

Oxidation of cardiolipin (CL) by its complex with cytochrome c (cyt c) plays a crucial role in triggering apoptosis. Through a combination of magnetic circular dichroism spectroscopy and potentiometric titrations, we show that both the ferric and ferrous forms of the heme group of a CL:cyt c complex exist as multiple conformers at a physiologically relevant pH of 7.4. For the ferric state, these conformers are His/Lys- and His/OH(-)-ligated. The ferrous state is predominantly high-spin and, most likely, His/-. Interconversion of the ferric and ferrous conformers is described by a single midpoint potential of -80 ± 9 mV vs SHE. These results suggest that CL oxidation in mitochondria could occur by the reaction of molecular oxygen with the ferrous CL:cyt c complex in addition to the well-described reaction of peroxides with the ferric form.     

Particle size dependence of the superconductivity and ferromagnetism in YBCO nanoparticles

Magnetic properties of superconducting yttrium barium copper oxide (YBa₂Cu₃O_7-δ) nanoparticles (31–43 nm) prepared by a chemical route have been studied. These nanoparticles have been found to clearly exhibit ferromagnetism at room temperature while superconducting transition is observed at lower temperatures. The low temperature hysteresis loops show evidence suggesting the presence of a large paramagnetic contribution in addition to the superconducting contributions from the particles. Bulk YBCO obtained by pelletizing and heating the same nanoparticles at a high temperature, displays the usual superconducting characteristics and gives no trace of ferromagnetism down to 10 K. The superconducting transition temperature of the nanoparticles is lower than for the bulk YBCO and there is a trend of decreasing T _c with smaller size of the particles. In contrast the ferromagnetic moment increases with decreasing particle size. The development of ferromagnetism is attributed to the presence of surface oxygen vacancies that lead to electron redistribution on the different ions at the surface. The simultaneous decrease of superconducting T _c and the increase of ferromagnetism with decreasing size considered as being reflective of the increased role of finite size and surface defects that weaken the superconductivity and enhance the ferromagnetism. Possible coexistence of surface ferromagnetism and bulk superconductivity at lower temperatures is discussed.     

A method of concurrent thermographic–photographic visualization of flow boiling in a minichannel

A method is developed to capture the distribution of surface temperature while simultaneously imaging the bubble motions in diabatic flow boiling in a horizontal minichannel. Liquid crystal thermography is used to obtain highly resolved surface temperature measurements on the uniformly heated upper surface of the channel. High-speed images of the flow field are acquired simultaneously and are overlaid with the thermal images. The local surface temperature and heat transfer coefficient can be analyzed with the knowledge of the nucleation site density and location, and bubble motion and size evolution. The horizontal channel is 1.2 mm high × 23 mm wide × 357 mm long, and the working fluids are Novec 649 and R-11. Optical access is through a machined glass plate which forms the bottom of the channel. The top surface is an electrically heated 76 μm-thick Hastelloy foil held in place by a water-cooled aluminum and glass frame. The heat loss resulting from this construction is computed using a conduction model in Fluent. The model is driven by temperature measurements on the foil, glass plate and aluminum frame. This model produces a corrected value for the local surface heat flux and enables the computation of the bulk fluid temperature and heat transfer coefficient along the channel. The streamwise evolution of the heat transfer coefficient for single-phase laminar flow is compared to theoretical values for a uniform-flux boundary condition. Examples of the use of the facility for visualizing subcooled two-phase flows are presented. These examples include measurements of the surface temperature distribution around active nucleation sites and the construction of boiling curves for locations along the test surface. Points on the curve can be associated with specific image sequences so that the role of mechanisms such as nucleation and the sliding of confined bubbles may be discerned.     

Appraisal of venomous metals in selected crops and vegetables from industrial areas of the Punjab Province

Due to the inadequate water sources, usually sewerage water and industrial effluents are being use for irrigation of the agricultural land around the industrial areas in Pakistan wherein crops and vegetables are cultivated. As untreated effluents contain heavy elements, toxic metals and organic pollutants that may find its way through food chain to general public and may cause health hazards. It is, therefore, mandatory to assess the toxic metals in such crops and vegetables. In this regard, samples of corn, millet, cabbage, spinach and potato were collected within the vicinity of industrial areas of the Faisalabad and Gujranwala regions. The food samples were analyzed using neutron activation analysis (NAA) technique. The highest concentration values of Arsenic (1.9 ± 0.1 μg/g) and Cobalt (0.85 ± 0.01 μg/g) were found in cabbage whereas Manganese (91.6 ± 0.2 μg/g), Antimony (0.15 ± 0.03 μg/g) and Selenium (1.1 ± 0.1 μg/g) were observed in spinach and Chromium (9.63 ± 1.3 μg/g) was found in millet crop. The observed concentrations of all the toxic and heavy metals in crops and vegetables are higher than those reported in the literature.     

A straightforward way to form close-packed TiO2 particle monolayers at an air/water interface

The aim of this study was to analyze if and how monolayers of TiO(2) particles could be directly formed at the air/water interface and if these monolayers could be transferred to a solid surface. TiO(2) particles with diameters of 300 nm, 500 nm, 1 μm, 5 μm, 10 μm, and 20 μm formed stable monolayers at pH 2. At low surface pressures, the particles formed small two-dimensional aggregates. Particles up to a radius of 5 μm displayed close packing at increased surface pressures. Particles of 10 μm radius formed a loose network, which is attributed to the strong adhesion caused by the weight-induced lateral capillary attraction. Every monolayer of particles could be transformed to a solid surface by the Langmuir-Blodgett deposition. At pH 6 or 11, the particles did not form stable monolayers at the air/water interface. They were instead dispersed in the aqueous phase and eventually sank to the bottom of the trough. At pH 11 the monolayer could, however, be stabilized by the addition of salt (0.5 M NaCl). The results are interpreted based on a changed wettability of the particles depending on pH and salt concentration.     

Kinetics studies on flavone glycosides: inhibitors of <Emphasis Type="Italic">α</Emphasis>-chymotrypsin

The mechanism of inhibition of α-chymotrypsin enzyme by two flavone glycosides having an apigenin basic skeleton, namely, apigenin 7-O-β-D(-6′′-p-E-coumaroyl)-glucoside (1) and apigenin 7-O-β-D-glucoside (2), isolated from Clematis orientalis L. (Ranunculaceae), was investigated. Lineweaver-Burk and Dixon plots and their secondary replots have shown that both of the compounds are competitive inhibitors of this enzyme. K_i values for 1 and 2 were found to be 71.75 ± 0.85 and 680.11 ± 0.11 μM, respectively.     

A novel fluorescent probe for NAD-consuming enzymes

A novel, fluorescent NAD derivative is processed as substrate by three different NAD-consuming enzymes. The new probe has been used to monitor enzymatic activity in a continuous format by changes in fluorescence and, in one case, to directly visualize alternative reaction pathways.     

Through the eye of an electrospray needle: mass spectrometric identification of the major peptides and proteins in the milk of the one‐humped camel (Camelus dromedarius)

The milk of the one‐humped camel (Camelus dromedarius) reportedly offers medicinal benefits, perhaps because of its unique bioactive components. Milk proteins were determined by (1) two‐dimensional gel electrophoresis and peptide mass mapping and (2) liquid chromatography–tandem mass spectrometry (LC–MS/MS) following one‐dimensional polyacrylamide gel electrophoresis. Over 200 proteins were identified: some known camel proteins including heavy‐chain immunoglobulins and others exhibiting regions of exact homology with proteins from other species. Indigenous peptides were also identified following isolation and concentration by two strategies: (1) gel‐eluted liquid fraction entrapment electrophoresis and (2) small‐scale electrophoretic separation. Extracts were analyzed by LC–MS/MS and peptides identified by matching strategies, by de novo sequencing and by applying a sequence tag tool requiring similarity to the proposed sequence, but not an exact match. A plethora of protein cleavage products including some novel peptides were characterized. These studies demonstrate that camel milk is a rich source of peptides, some of which may serve as nutraceuticals. Copyright © 2013 John Wiley & Sons, Ltd.