Engineered alginate hydrogels for effective microfluidic capture and release of endothelial progenitor cells from whole blood

Microfluidic adhesion-based cell separation systems are of interest in clinical and biological applications where small sample volumes must be processed efficiently and rapidly. While the ability to capture rare cells from complex suspensions such as blood using microfluidic systems has been demonstrated, few methods exist for rapid and nondestructive release of the bound cells. Such detachment is critical for applications in tissue engineering and cell-based therapeutics in contrast with diagnostics wherein immunohistochemical, proteomic, and genomic analyses can be carried out by simply lysing captured cells. This paper demonstrates how the incorporation of four-arm amine-terminated poly(ethylene glycol) (PEG) molecules along with antibodies within alginate hydrogels can enhance the ability of the hydrogels to capture endothelial progenitor cells (EPCs) from whole human blood. The hydrogel coatings are applied conformally onto pillar structures within microfluidic channels and their dissolution with a chelator allows for effective recovery of EPCs following capture.     

Recyclable heterogeneous copper oxide on alumina catalyzed coupling of phenols and alcohols with aryl halides under ligand-free conditions

An efficient alumina-supported CuO-catalyzed O-arylation of phenols and aliphatic alcohols with various aryl as well as heteroaryl halides under ligand-free conditions are reported. This protocol provides a variety of diaryl ether and bis-diaryl ether motifs by reacting different aryl/aliphatic halides with differently substituted phenols and saturated alcohols in the presence of a catalytic amount of CuO on alumina and KOH as a base at moderate temperature under nitrogen atmosphere. The described methodology is simple, straightforward and efficient to afford the cross-coupled products in high yields under ligand-free conditions. The explored catalyst is inexpensive, air-stable and recyclable up to three cycles.     

Application of ethyl chloroformate derivatization for solid-phase microextraction–gas chromatography–mass spectrometric determination of bisphenol-A in water and milk samples

A simple and rapid analytical method based on in-matrix ethyl chloroformate (ECF) derivatization has been developed for the quantitative determination of bisphenol-A (BPA) in milk and water samples. The samples containing BPA were derivatised with ECF in the presence of pyridine for 20 s at room temperature, and the non-polar derivative thus formed was extracted using polydimethylsiloxane solid-phase microextraction (SPME) fibres with thicknesses of 100 μm followed by analysis using gas chromatography–mass spectrometry. Three alkyl chloroformates (methyl, ethyl and isobutyl chloroformate) were tested for optimum derivatisation yields, and ECF has been found to be optimum for the derivatisation of BPA. Several parameters such as amount of ECF, pyridine and reaction time as well as SPME parameters were studied and optimised in the present work. The limit of detection for BPA in milk and water samples was found to be 0.1 and 0.01 μg L⁻¹, respectively, with a signal-to-noise ratio of 3:1. The limit of quantitation for BPA in milk and water was found to be 0.38 and 0.052 μg L⁻¹, respectively, with a signal-to-noise ratio of 10:1. In conclusion, the method developed was found to be rapid, reliable and cost-effective in comparison to silylation and highly suitable for the routine analysis of BPA by various food and environmental laboratories.     

Effect of viscous dissipation on natural convection heat and mass transfer from vertical cone in a non-Newtonian fluid saturated non-Darcy porous medium

In this paper we investigate the influence of viscous dissipation and Soret effect on natural convection heat and mass transfer from vertical cone in a non-Darcy porous media saturated with non-Newtonian fluid. The surface of the cone and the ambient medium are maintained at constant but different levels of temperature and concentration. The Ostwald-de Waele power law model is used to characterize the non-Newtonian fluid behavior. The governing equations are non-dimensionalized into non-similar form and then solved numerically by local non-similarity method. The effect of non-Darcy parameter, viscous dissipation parameter, Soret parameter, buoyancy ratio, Lewis number and the power-law index parameter on the temperature and concentration field as well as on the heat and mass transfer coefficients is analyzed.     

Synthesis of Allyl Aryl Sulfone Derivatives from Baylis?Hillman Acetates in Water

Various phenyl and p‐tolyl allyl sulfone derivatives were prepared stereoselectively by reacting Baylis? Hillman acetates with sodium 4‐R‐benzenesulfinate (R=H, Me) in H₂O. The reaction was very efficient in providing the corresponding sulfone derivatives in good to excellent yields (Table).     

Dual solutions in mixed convection with variable physical properties

Assisting and opposing flows in a mixed convection boundary layer flow over an isothermal vertical plate are studied for the case of variable physical properties and uniform free stream. Fluid viscosity and thermal conductivity are assumed to be linear functions of temperature. Using local similarity the flow and heat transfer quantities are found to be functions of four parameters, i.e. Richardson number, Prandtl number, a viscosity variation parameter and a thermal conductivity variation parameter. Numerical solutions are obtained by two methods, a shooting technique and Nachtsheim-Swigert technique, for selected values of parameters appropriate for the fluids considered and specific temperatures of the plate and ambient fluid. For assisting flows, there exist solutions for all values of Richardson number while for opposing flows solutions exist only for a finite set of its values and, in addition, there also exist dual solutions. Important flow and heat transfer quantities of practical interest are determined and the influence of different parameters is discussed.     

Synthesis and characterization of Eu doped SrY2O4 phosphor

The present paper reports that TL glow curve and kinetic parameter of Eu³⁺ doped SrY₂O₄ phosphor irradiated by beta source. Sample was prepared by solid state preparation method. Sample was characterized by XRD analysis and particle size was calculated by Debye–Scherrer formula. The sample was irradiated with Sr-90 beta source giving a dose of 10 Gy and the heating rate used for TL measurements are 6.7 °C/s. The samples display good TL peaks at 106 °C, 225 °C and 382 °C. The corresponding kinetic parameters are calculated. The photoluminescence excitation spectrum at 247 and 364 nm monitored with 400 nm excitation and the corresponding emission peaks at 590, 612 and 624 nm are reported.     

An interactive decision support system for the bank courier routing problem

In this paper, we present an interactive decision support system for collecting and processing financial transit material for the Southeast Bank N.A. The underlying model is a bicriteria shortest path problem. The system provides a feasible solution in a matter of minutes, which provides the decision maker with an opportunity to perform “what if” analysis. The system can be extended to other vehicle routing problems with inventory components.     

Thermal dispersion effects on non-Darcy natural convection over horizontal plate with surface mass flux

Summary The effect of surface mass flux on the non-Darcy natural convection over a horizontal flat plate in a saturated porous medium is studied using similarity solution technique. Forchheimer extension is considered in the flow equations. The suction/injection velocity distribution has been assumed to have power function form Bx ^l , similar to that of the wall temperature distribution Ax ⁿ , where x is the distance from the leading edge. The thermal diffusivity coefficient has been assumed to be the sum of the molecular diffusivity and the dynamic diffusivity due to mechanical dispersion. The dynamic diffusivity is assumed to vary linearly with the velocity component in the x direction, i.e. along the hot wall. For the problem of constant heat flux from the surface (n=1/2), similarity solution is possible when the exponent l takes the value −1/2. Results indicate that the boundary layer thickness decreases whereas the heat transfer rate increases as the mass flux parameter passes from the injection domain to the suction domain. The increase in the thermal dispersion parameter is observed to favor the heat transfer by reducing the boundary layer thickness. The combined effect of thermal dispersion and fluid suction/injection on the heat transfer rate is discussed. Received 7 December 1995; accepted for publication 7 January 1997     

Thermal dispersion effects on non-Darcy natural convection with lateral mass flux

The method of similarity solution is used to study the influence of lateral mass flux and thermal dispersion on non-Darcy natural convection over a vertical flat plate in a fluid saturated porous medium. Forchheimer extension is considered in the flow equations and the coefficient of thermal diffusivity has been assumed to be the sum of molecular diffusivity and the dispersion thermal diffusivity due to mechanical dispersion. The suction/injection velocity distribution has been assumed to have power function form Ax ^l , where x is the distance from the leading edge and the wall temperature distribution is assumed to be uniform. When l=−1/2, similarity solution is possible, and the results indicate that the boundary layer thickness decreases where as the heat transfer rate increases as the mass flux parameter passes from injection domain to the suction domain. The increase in the thermal dispersion parameter is observed to enhance the heat transfer. The combined effect of thermal dispersion and fluid suction/injection on the heat transfer rate is discussed. Received on 9 September 1996