In the present research, we have investigated a drug delivery system based on the pH‐responsive behaviors of zein colloidal nanoparticles coated with sodium caseinate (SC) and poly ethylene imine (PEI). These systematically designed nanoparticles were used as nanocarriers for encapsulation of ellipticine (EPT), as an anticancer drug. SC and PEI coatings were applied through electrostatic adsorption, leading to the increased size and improved polydispersity index of nanoparticles as well as sustained release of drug. Physicochemical characteristics such as hydrodynamic diameter, size distribution, zeta potential and morphology of nanoparticles prepared using different formulations and conditions were also determined. Based on the results, EPT was encapsulated into the prepared nanoparticles with a high drug loading capacity (5.06%) and encapsulation efficiency (94.8%) under optimal conditions. in vitro experiments demonstrated that the release of EPT from zein‐based nanoparticles was pH sensitive. When the pH level decreased from 7.4 to 5.5, the rate of drug release was considerably enhanced. The mechanism of pH‐responsive complexation in the drug encapsulation and release processes was extensively investigated. The pH‐dependent electrostatic interactions and drug state were hypothesized to affect the release profiles. Compared to the EPT‐loaded zein/PEI nanoparticles, the EPT‐loaded zein/SC nanoparticles exhibited a better drug sustained‐release profile, with a smaller initial burst release and longer release period. According to the results of in vitro cytotoxicity experiments, drug‐free nanoparticles were associated with a negligible cytotoxicity, whereas the EPT‐loaded nanoparticles displayed a high toxicity for the cancer cell line, A549. Our findings indicate that these pH‐sensitive protein‐based nanoparticles can be used as novel nanotherapeutic tools and potential antineoplastic drug carriers for cancer chemotherapy with controlled release. 相似文献
Monatshefte für Chemie - Chemical Monthly - In the present study, multi-spectroscopic methods were applied to investigate the effect of NiO nanoparticles on the structure, activity, and... 相似文献
The essential oils from aerial parts and fruits of Anisosciadium orientale DC. growing wild in Iran were obtained by hydrodistillation and analyzed by GC and GC/MS. Seventy-one compounds were identified in the fruit oil and fifteen in the oil from the aerial parts. The main oil components of the fruits and aerial parts were myristicin (33.5%-33.7%), alpha-terpinolene (22%-25.8%) and limonene (19.5%-19.7%). Some compounds, such as geranyl butyrate and germacrene-D, were only detected in the fruit oil. 相似文献
Micromeria persica Boiss. is medicinal and aromatic plant, belonging to the Lamiaceae family. The chemical composition of the essential oils (EOs) from aerial parts of M. persica were extracted using hydro-distillation method and analysed using GC and GC–MS. Fifty-two compounds were identified in the EOs of aerial parts of M. persica. The main chemical compositions were n-hexadecanoic acid (14.9%), thymol (9.5%), linoleic acid (8.0%), carvacrol (5.6%), (E)-nerolidol (5.5%), linolenic acid (5.5%), α-cadinol (2.7%), linalool (2.7%), borneol (2.6%), caryophyllene oxide (2.3%) and pulegone (2.0%). Presence of borneol, thymol, carvacrol and pulegone suggests the potential of this plant as a flavouring source in the food industry, being used in perfumery and cosmetics industry, vitamin E synthesis and exhibit strong fungicidal, antibacterial and antimicrobial activities. 相似文献
Lagoecia cuminoides L. belongs to the family of Umbelliferae (Apiaceae), and known also as common wild cumin. The aerial parts of L. cuminoides were collected at the flowering stage and dried, then the methanolic extract was analyzed for polyphenol compounds identified by HPLC-DAD and antioxidant activity (DPPH(2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay). It was found that the predominant phenolic constituents were chlorogenic acid, hesperidin, rosmarinic acid, hesperetin and vanillin. The antioxidant activity of methanolic extract from L. cuminoides was found 1597 μg/mL in DPPH scavenging assay. There is no strict positive relationship between the polyphenolic content and antioxidant activity of extracts. 相似文献
Construction and feature of a nanocomposite modified carbon paste electrode for aluminum(III) ion determination based on N,N′-dipyridoxyl (1,2-cyclohexanediamine) (PYCA) as a novel selector material will be covered by this paper. The optimum composition, Nernstian slope/linear range/detection limit in the forms of calibration graph, response time, utilizable pH range, repeatability and precision of measurements of the aluminum(III) ion using the new fabricated Al3+-CPE was evaluated. The optimal composition which performed over Al+3 ion concentration range 1.0 × 10?8 mol L?1–1.0 × 10?1 mol L?1 with near-Nernstian slope equal 20.9 ± 0.2 mV decade?1 and low detection limit about 5.0 × 10?9 mol L?1, was formed of ionophore (PYCA 3 %), binder (paraffin oil 30 %), modifier [multi-wall carbon nanotubes (MWCNTs) 1 %] & [Nanosilica (NS) 0.5 %], and inert matrix (graphite powder 65.5). The request time to give rise Nernstian response of electrode for concentrations from 1.0 × 10?8 mol L?1 to 1.0 × 10?1 mol L?1 of Al3+ ion solution was estimated about ~6 s. The new Al3+-CPE was applied in pH range 2.0–5.0 with no consequential change in potential response. To verify the selectivity of electrode toward aluminum(III) ion in the presence of different metallic cations, matched potential method was used. The obtain results in analytical applications reflect the excellent ability of this electrode to play the role as endpoint indicator electrode in both titration and direct potentiometric measurements. 相似文献
Here, the synthesis of photochromic hydrogen bond-assembled [2]rotaxanes using bis-fumarate as a thread for the first time is reported. In fact, photochromic 1,3-diazabicyclo[3.1.0]hex-3-ene moieties were used as stoppers and two-atom spacers managed good binding sites for the tetralactam macrocycles in clipping reactions. Moreover, the yields of photochromic [2]rotaxanes highly depended on the NO2 substituent stoppers. While the thread with a para –NO2 substituent as stopper units was shown to be an excellent template for the synthesis of photochromic [2]rotaxanes. The structures of the [2]rotaxanes are established clearly in solution by chemical shifts of the 1H 13C NMR signals and UV–Vis spectra. A pronounced bathochromic shift was occurred in the excitation wavelength of photochoromic [2]rotaxanes compared with the absorption band of photochromic threads. Therefore, these organizations can be applied in light-driven molecular switches and motors. The reversible transformation of trans and cis geometric photoisomers under UV radiation was identified. In other efforts, the possibility of the process of trans to cis interconversion of the fumarate linker under UV irradiation has been examined computationally and it has appeared that it may cause the transverse of the bis-fumarate linker inside the tetralactam macrocycle to some extent.
The adsorption of the dyes Acid Red 114 and Reactive Black 5 in aqueous solutions on polyhydroxyl dendrimer magnetic nanoparticles (Fe3O4@SiO2‐TRIS) was studied in a batch system. The Fe3O4@SiO2‐TRIS NPs were characterized by Fourier transform infrared spectroscopy, X‐ray diffraction, and transmission electron microscopy. Experiments were performed under different conditions such as the initial dye concentration, adsorbent dose, and pH. The pseudo‐second‐order model provided a very good fit for the two anionic dyes. The Langmuir and Freundlich adsorption models were used to describe the equilibrium isotherms at different temperatures, and the former agreed very well with the experimental data. However, the adsorption capacity of Fe3O4@SiO2‐TRIS NPs was reduced during surface modification, which could be due to the dye occupying the binding sites of the dendrimer. Thermodynamic parameters, namely the change in free energy (ΔG0), enthalpy (ΔH0), and entropy (ΔS0), were also determined. 相似文献