Rapid and reproducible capillary electrophoretic separation of double-stranded DNA fragments in a simple methyl cellulosic sieving system

Summary A rapid, robust and reproducible method providing excellent separation performance and simplicity using a 0.5% MC-4000 methyl cellulosic sieving medium in DB-1 coated capillaries has been developed. The method is suitable for qualitative comparison of DNA restriction profiles for fragments in the size range 100–1000 base pairs (bp). Efficiencies up to 8.5 million plates/m (1057 bp fragment) were recorded. Peak resolution of 6 bp (291/297 bp, 335/341 bp) and 4 bp (238/242 bp, 341/345 bp) was achieved. In addition, 1 bp partial resolution of 123/124 bp and 298/297 bp was obtained. Run-to-run (n=15), day-to-day (n=4), and capillary-to-capillary (n=3) variations of 0.1–0.2% RSD, 0.3–0.5% RSD, and 0.1–0.3% RSD, respectively, were observed. The MC-4000 sieving matrix was found to be better than hydroxypropyl methyl cellulose and hydroxypropyl cellulose, in terms of both performance and stability in the DB-1 coated capillaries. The efficiency and resolution in DB-WAX capillaries were inferior to those obtained in DB-1 capillaries. The commercially available DB-1 capillaries were stable for months in the sieving medium at pH 8.3 and could be regenerated to provide high efficiency after accidental current breaks.     

Inclusive production of neutral vector mesons in hadronic Z decays

A data sample of about 3.0 million hadronicZ decays collected by the ALEPH experiment at LEP in the years 1991 through 1994 is used to make an inclusive selection of B hadron events. In this event sample 4227±140±252B* mesons in the decayB*→Bγ and 1944±108±161B** _u,d mesons decaying into a B meson and a charged pion are reconstructed. Here and in the followingB** _u,d denotes the eightL=1(bū) and (bd) states and their charge conjugate. For the well establishedB* meson the following quantities are obtained:ΔM=M _B*−M_B=(45.30±0.35±0.87) MeV/c² andN _B*/(N _B+N _B*)=(77.1±2.6±7.0)%. The angular distribution of the photons in theB* rest frame is used to measure the relative contribution of longitudinalB* polarization states to beσ _L/(σ _L+σ _T)=(33±6±5)%.     

A PULSED LASER AND PULSE RADIOLYSIS STUDY OF AMPHIPHILIC CHLOROPHYLL DERIVATIVES WITH PDT ACTIVITY TOWARD MALIGNANT MELANOMA

Two amphiphilic derivatives of chlorophyll, which have high potential as photodynamic therapy sensitizers for malignant melanoma have been investigated by a combination of laser flash photolysis and pulse radiolysis. It is shown that direct excitation of monomeric forms of these molecules in both hydrophilic and hydrophobic environments produces significant yields of the corresponding triplet states, which have been characterized in terms of spectral and kinetic parameters. In both environments, scavenging of the triplets by oxygen produces singlet oxygen, O₂(^lΔ₈), with essentially unit efficiency as evidenced by time-resolved IR luminescence measurements.     

Synthesis of furaxanenitrolic acids

Previously unreported furoxanenitrolic acids have been synthesized.Translated from Khimiya Geterotsiklicheskikh Soedinenii, No. 8, pp. 1117–1119, August, 1993.     

About non-linear realization of quantumSL q(2, R) group

Published in Teoreticheskaya i Matematicheskaya Fizika, Vol. 95, No. 2, pp. 183–186, May, 1993.     

On the Finiteness of the Discrete Spectrum of a Four-Particle Lattice Schrödinger Operator

A Hamiltonian describing four bosons that move on a lattice and interact by means of pair zero-range attractive potentials is considered. A stronger version of the Hunziker–Van Vinter–Zhislin theorem on the essential spectrum is established. It is proved that the set of eigenvalues lying to the left of the essential spectrum is finite for any interaction energy of two bosons and is empty if this energy is sufficiently small.     

Crystal structures of two ancylite modifications

The crystal structures of two ancylite specimens from Khibiny massif (the Kola Peninsula, Russia)—ancylite-(Ce) from alkali hydrothermalites (Sr_1.01Ca_0.02Ba_0.01)_Σ1.04(Ce_0.52La_0.28Nd_0.11Pr_0.04 Sm_0.01)_Σ0.96(CO₃)₂(OH_0.83F_0.13)_Σ0.96 · 0.9H₂O and ancylite-(Ce) from carbonatites—have (Sr_0.80Ca_0.05Ba_0.01)_Σ0.86(Ce_0.62La_0.40Nd_0.09Pr_0.03) _Σ1.14(CO₃)₂(OH_0.99F_0.15)_Σ1.14 · 1.0H₂O been refined by the Rietveld method. A focusing STOE-STADIP diffractometer with a bent Ge(111) primary monochromator was used (λ MoK _{α 1} radiation, 2.16° < 2θ < 54.98°; reflection number 237–437). All the computations for ancylite from alkali hydrothermalites were performed within the sp. gr. Pmc2₁, a = 5.0634(1) Å, b = 8.5898(1) Å, c = 7.2781(1) Å, V = 316.55(1) ų, R _wp = 1.90; the computations for ancylite from carbonatites were performed within the sp. gr. Pmcn, a = 5.0577(1) Å, b = 8.5665(2) Å, c = 7.3151(2) Å, V = 316.94(1) ų, R _wp = 2.38 in the anisotropic approximation of thermal vibrations of cations and oxygen atoms.     

Nuclear equation of state and compression modes

The nuclear matter (N = Z and no Coulomb interaction) incompressibility coefficient, K _nm , which is directly related to the curvature of the nuclear matter equation of state, is a very important physical quantity in the study of properties of nuclei, supernova collapse, neutron stars and heavy-ion collisions. We review the current status of K _nm and the experimental and theoretical methods used to determine the value of K _nm from the excitation crosssections σ(E) and the transition strength distributions S(E) of compression modes in nuclei. In particular, we will consider the isoscalar giant monopole resonance (ISGMR) and the isoscalar giant dipole resonance (ISGDR) and provide a simple explanation to the long standing problem of the conflicting results obtained for K _nm , deduced from experimental data on excitation cross sections for the ISGMR and data for the ISGDR.     

Laser writing techniques for photomask fabrication using a femtosecond laser

Photomasks are the backbone of microfabrication industries. Currently they are fabricated by a lithographic process, which is very expensive and time consuming since it is a multi-step process. These issues can be addressed by fabricating photomasks by direct femtosecond laser writing, which is a single-step process and comparatively cheaper and faster than lithography. In this paper we discuss our investigations on the effect of two types of laser writing techniques, namely front- and rear-side laser writing, with regard to the feature size and the edge quality of a feature. It is proved conclusively that for the patterning of masks, front-side laser writing is a better technique than rear-side laser writing with regard to smaller feature size and better edge quality. Moreover the energy required for front-side laser writing is considerably lower than that for rear-side laser writing. Received: 22 May 2001 / Accepted: 14 September 2001 / Published online: 17 October 2001