全文获取类型
收费全文 | 173篇 |
免费 | 14篇 |
专业分类
化学 | 171篇 |
晶体学 | 1篇 |
数学 | 1篇 |
物理学 | 14篇 |
出版年
2021年 | 4篇 |
2020年 | 6篇 |
2019年 | 8篇 |
2018年 | 1篇 |
2016年 | 7篇 |
2015年 | 4篇 |
2014年 | 5篇 |
2013年 | 13篇 |
2012年 | 17篇 |
2011年 | 16篇 |
2010年 | 3篇 |
2009年 | 5篇 |
2008年 | 5篇 |
2007年 | 9篇 |
2006年 | 13篇 |
2005年 | 14篇 |
2004年 | 8篇 |
2003年 | 11篇 |
2002年 | 7篇 |
2001年 | 7篇 |
2000年 | 4篇 |
1999年 | 6篇 |
1998年 | 1篇 |
1996年 | 3篇 |
1992年 | 1篇 |
1991年 | 2篇 |
1989年 | 3篇 |
1986年 | 1篇 |
1985年 | 2篇 |
1982年 | 1篇 |
排序方式: 共有187条查询结果,搜索用时 281 毫秒
31.
A Reversible Fluorescent Probe for Real‐Time Live‐Cell Imaging and Quantification of Endogenous Hydropolysulfides 下载免费PDF全文
Dr. Keitaro Umezawa Dr. Mako Kamiya Prof. Dr. Yasuteru Urano 《Angewandte Chemie (International ed. in English)》2018,57(30):9346-9350
The chemical biology of reactive sulfur species, including hydropolysulfides, has been a subject undergoing intense study in recent years, but further understanding of their “intact” function in living cells has been limited owing to a lack of appropriate analytical tools. In order to overcome this limitation, we developed a new type of fluorescent probe that reversibly and selectively reacts to hydropolysulfides. The probe enables live‐cell visualization and quantification of endogenous hydropolysulfides without interference from intrinsic thiol species such as glutathione. Additionally, real‐time reversible monitoring of oxidative‐stress‐induced fluctuation of intrinsic hydropolysulfides has been achieved with a temporal resolution on the order of seconds, a result which has not yet been realized using conventional methods. These results reveal the probe's versatility as a new fluorescence imaging tool to understand the function of intracellular hydropolysulfides. 相似文献
32.
N. Shigeta H. Matsuoka A. Osa M. Koizumi M. Izumo K. Kobayashi K. Hashimoto T. Sekine R. M. Lambrecht 《Journal of Radioanalytical and Nuclear Chemistry》1996,205(1):85-92
No-Carrier-Added186Re was produced using the186W(p,n)186Re nuclear reaction with 13.6 MeV protons on thick targets of 99.79% isotopically enriched186WO3. The theoretical excitation functions for producing186Re, and possible radionuclidic impurities of182Re,183Re, and184Re were calculated using the ALICE code. Cross-sections of the186W(p,n)186Re reaction were measured up to 20 MeV using the stacked target method with thin foils of natural composition tungsten metal. The experimental and theoretical excitation functions were in good agreement. Targetry used at the TIARA cyclotron, and a radiochemical separation scheme for186Re are described. 相似文献
33.
Novel fluorescent Mg2+ probes, 2'-carboxyfluorescein (2'-CF) and its derivatives, that are excitable by visible light, were designed, synthesized and characterized. After complexation with Mg2+, the absorption and emission maxima of these fluorescent probes were red-shifted and the fluorescence intensity was increased 11-fold at neutral pH. The apparent dissociation constant (Kd) of the Mg2+-2'-CF complex was 15.8 mM at neutral pH. The Kd of the Ca2+-2'-CF complex was about 10 times larger than that of the Mg2+ complex and the other derivatives showed similar characteristics. In contrast, all the commercially available fluorescent Mg2+ probes have a higher affinity for Ca2+ than Mg2+. 2'-CF fluoresced in alkaline solution (pH > 8) and the pK, value was 8.8. The pKa value of the Mg2+-2'-CF complex was 6.8, and the fluorescence intensity was increased in the neutral conditions. Thus, the addition of Mg2+ resulted in a lowering of the pKa, and also an increase of the fluorescence intensity. 相似文献
34.
Separation of the enantiomers and diastereomers of ephedrines was investigated for impurity profiling of methamphetamine. We describe a method for the analysis of (1S,2R)-(+)-ephedrine, (1R,2S)-(-)-ephedrine, (1S,2S)-(+)-pseudoephedrine and racemic methylephedrine in bulk methamphetamine by HPLC using two different columns: a phenyl-beta-cyclodextrin-type column and an ODS-type column. The analytes were detected by UV absorbance measurement at 210 nm. As little as 0.05% of each ephedrine in bulk methamphetamine could be determined. In the impurity profiling of methamphetamine, the identification of ephedrines may provide valuable information about the precursor. This method was confirmed to be sufficiently sensitive to identify trace amounts of (1R,2S)-(-)-ephedrine and (1S,2S)-(+)-pseudoephedrine in bulk methamphetamine synthesized by the Emde method. 相似文献
35.
Kawabata E Kikuchi K Urano Y Kojima H Odani A Nagano T 《Journal of the American Chemical Society》2005,127(3):818-819
Zinc (Zn2+) is found in every cell in human bodies. A few millimolar of free Zn2+ exists in the vesicles of presynaptic neurons in the mammalian brain and is released by synaptic activity or depolarization, modulating the function of certain ion channels and receptors. Although various chemical tools for measuring Zn2+ in biological samples, such as fluorescent probes for Zn2+, have been developed, Zn2+-selective chelators have room to be improved. Research on Zn2+ signals in the brain has traditionally employed several chelators, which have several shortcomings for biological applications. Here we report the design, synthesis, and properties of new membrane-impermeable chelators selective for Zn2+ and describe biological applications in hippocampal slices. As a result, our newly designed chelator revealed the first biological implication that presynaptic Zn2+ can be released in the CA1 region. This confirms the utility of these new chelatotrs as extracellular Zn2+ chelators for biological applications. 相似文献
36.
Refractive indices of organo‐metallic and ‐metalloid compounds: A long‐range corrected DFT study 下载免费PDF全文
Shintaro Maekawa Krzysztof Moorthi Yasuteru Shigeta 《Journal of computational chemistry》2016,37(32):2759-2769
Refractive indices of metal‐ and metalloid‐containing compounds are systematically evaluated using the Lorentz–Lorenz equation with polarizabilities obtained via density functional theory (DFT). Among exchange‐correlation functionals studied, the long‐range corrected (LC) fuctionals yield the lowest errors for the polarizabilities of gaseous compounds and refractive indices of liquids. The LC‐DFT predicts very well the wavelength dependence of refractive indices. A scheme for computing Abbe numbers of organometallic and organometaloid compounds is proposed and a refractive index – Abbe number plot for 80 compounds is constructed. The compounds containing heavier metals tend to have higher refractive index and lower Abbe number, but several outliers, among them Te(CH3)2, Ni(PF3)4, Sb(C2F3)3, Hg(C2F3)2, are found. For Hg(C2F3)2, also the effect of intramolecular and intermolecular degrees of freedom on polarizability is investigated. The absolute relative error in polarizability decreases from 5.7% for monomer model to 1.7% when a dimer model (derived from the available experimental crystal data) is employed. © 2016 Wiley Periodicals, Inc. 相似文献
37.
38.
39.
Ryuhei Harada Ryunosuke Yoshino Hiroaki Nishizawa Yasuteru Shigeta 《Journal of computational chemistry》2019,40(15):1530-1537
Outlier flooding method (OFLOOD) is an efficient conformational sampling method developed by the authors. In the present study, to further enhance the conformational sampling efficiency, a set of parameters (temperatures and pressures) specified as inputs in the original OFLOOD were shuffled before restarting the short-time molecular dynamics (MD) simulations. Because of the diversity of these parameters, it was confirmed that the extended OFLOOD becomes superior to the original one in finding the folding pathways of Trp-cage. © 2019 Wiley Periodicals, Inc. 相似文献
40.
Ranko Motoshige Yasuteru Mawatari Asahi Motoshige Yoshiaki Yoshida Takahiro Sasaki Hiroaki Yoshimizu Tomoyuki Suzuki Yoshiharu Tsujita Masayoshi Tabata 《Journal of polymer science. Part A, Polymer chemistry》2014,52(6):752-759
The yellow‐colored poly(phenylacetylene), Poly( Y ), is obtained from phenylacetylene using a [Rh(nbd)Cl]2‐NEt3 catalyst in ethanol at 25 °C. The color of Poly( Y ) drastically changes into red Poly( R ) or reddish‐black Poly( B ) by immersion in acetylacetone or exposure to chloroform vapor, respectively. Poly( R ) is also created from Poly( B ) by contact with acetylacetone. Poly( Y ) is regenerated from both Poly( R ) and Poly( B ) by reprecipitation from their chloroform solution into methanol. Wide‐angle X‐ray scattering (WAXS) patterns of Poly( Y ) and Poly( R ) correspond to a pseudohexagonal crystal called a columnar as stretched cis‐transoid and contracted cis‐cisoid helices, respectively. These helical diameters and pitch widths obtained from the WAXS measurements are agreed with those of MMFF94 calculation models. The smallest helical pitch width is 3.3 Å for Poly( R ) and Poly( B ). Moreover, information regarding the size and ordering of the vacant space within each polymer is estimated by using 129Xe NMR technique. © 2013 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2014 , 52, 752–759 相似文献