Selective foam separation of binary protein solution by SDS complexation method

A fundamental study about the selective foam separation of protein mixture was carried out. A solution containing two proteins, ovalbumin (OA) and lysozyme (LZ), and an anionic surfactant, sodium dodecyl sulfate (SDS), was adjusted to pH 6.0, which referred to an intermediate state between the isoelectric points of the proteins. The solution was processed by continuous foam separation. The results showed that a proper addition of SDS greatly improved the selective recovery of LZ to OA. The experimental data were well explained by a simple model that most of cationic protein molecules (LZ) are associated with SDS and the adsorption of all the species including LZ-SDS complexes are subjected to Langmuir adsorption isotherm. The results also showed that one of the Langmuir parameters, which means a kind of lyophillic property of adsorbed material, of LZ-SDS complexes was extremely large as compared with that of primary protein.     

Enantio- and diastereoselective, stereospecific mannich-type reactions in water

Catalytic asymmetric Mannich-type reactions in water proceeded in high yields and selectivities using a combination of ZnF2 and a chiral diamine that has the methoxy groups on the aromatic rings. In these reactions, either syn- or anti-Mannich adducts were stereospecifically obtained from (E)- or (Z)-silicon enolate, in contrast with most asymmetric Mannich-type reactions.     

特征线法在求解非线性液相吸附色谱模型中的应用

本文应用特征线法求解非线性液相吸附色谱分离过程模型，论述了特征线法的原理和步骤，讨论了时间步长和空间步长对模型数值解的影响，并用色谱分离甘露醇和山梨醇以及分离蔗糖和还原糖的实验进行验证，用特征线法计算的理论流出曲线与实验流出线吻合较好。本文还分折了模型中各个参数的灵敏度，结果表明：吸附等温方程Q＝Ci*／（ai＋biCi*）中参数ai比参数bi以及总传质系数ki有更高的灵敏度。     

二碘化钐促进的硒代酯的合成

用SmI2/THF/HMPA体系在室温下使二芳基二硒醚中的Se-Se键还原断裂成硒负离子物种, 继而与酸酐作用得到硒代酯, 该反应条件温和中性, 产率良好, 提供了合成硒代酯的一个新途径。     

偏氟乙烯-三氟氯乙烯共聚物链结构的研究

用裂解气相色谱-质谱法(Py-GC/MS)研究了单体克分子比近于1:1的偏氟乙烯-三氟氯乙烯共聚物(F23)链结构。采用了居里点裂解器和裂解探针。质谱的鉴定结果表明,两种裂解器在一定条件下得到的F23裂解产物非常相似。根据这些裂解产物的化学结构,可以推断F23中两种单体的键接方式,并确定F23中占优势的链段排列为:(?)     

Simultaneous determination of triterpene saponins in ginseng drugs by high-performance liquid chromatography

A HPLC method for the simultaneous determination of 11 triterpene saponins with four-type aglycones (protopanaxadiol, protopanaxatriol, ocotillol and oleanolic acid types) in Ginseng drugs was developed and validated. Using a gradient of acetonitrile and 10 mM K-phosphate buffer (pH 5.80) as the mobile phase and UV detection at 196 nm, more than 18 ginsenosides with different aglycones were separated satisfactorily within 60 min. The detection limits (signal/noise> or =3) were 0.1 microg for ginsenosides Rb1, Rc, Rd, Re and Rg1, chikusetsusaponin III, and notoginsenoside R2, 0.2 microg for gisenoside Ro and chikusetsusaponin IVa, 0.3 microg for chikusetsusaponin IV, and 3 microg for majonoside R2. The calibration curve of each saponin had a correlation coefficient close to 1. Intra- and interday precisions were less than 2.1% (n=5) and 3.3% (n=15), respectively. The recovery rates of extraction were in the range of 96.4-102.7% for all ginsenosides. By adopting this method, the determinations of 11 ginsenosides in three Ginseng drugs derived from Panax ginseng, Panax vietnamensis var. fuscidiscus and Panax japonicus (Japan) were achieved.     

5－（4－氯苯基）－10，15，20－三苯基卟啉及其配合物的合成、结构表征及热分解机理

合成了标题卟啉配体及其钴、镍、铜、锌和铁的五种配合物．对它们进行了元素分析、ＵＶ、ＩＲ和荧光光谱等结构表征．用ＤＴＡ、ＴＧ、ＤＳＣ等手段研究了这些新化合物的热稳定性及热分解机理，实验结果与ＨＭＯ方法计算的结果一致．     

三元配合物钯（Ⅱ）-2,2''-联吡啶-L-天冬氨酸与DNA作用研究

The ternary complex Pd(Ⅱ)-2,2‘-bipyridine-L-asparagic acid was synthesized and characterized by elemental analysis, IR-spectra and molar conductance. The formula of the complex is Pd(bipy)(L-asp). The interaction of the complex with DNA has been studied by UV-spectra, fluorescence spectra, CD-spectra and gel electrophoresis. The results showed that the interaction of the complex with DNA performed mainly in intercalative mode and the extent of interaction was dependent on the concentration of the complex.