Radiolabeling of lipo-chitooligosaccharides using the NodH sulfotransferase: a two-step enzymatic procedure

Background  

The NodH sulfotransferase from Sinorhizobium meliloti has been used to radiolabel lipochitooligosaccharidic (LCO) Nod factor signals with ³⁵S from inorganic sulfate in a two-step enzymatic procedure. The first step involved the production of 3'-phosphoadenosine 5'-phosphosulfate (PAPS), a sulphate donor, using enzymes contained in a yeast extract, and the second step used the NodH enzyme. However with this established procedure, only a low incorporation of the initial inorganic sulfate into the Nod factors was obtained (about 7% after purification of the labeled compounds). The aim of this work was to optimize the radiolabelling of Nod factors with ³⁵S.     

Cloud point of aqueous solutions of tetrabutylammonium dodecyl sulfate is a function of the concentration of counterions in the aqueous phase

The cloud point of the surfactant tetrabutylammonium dodecyl sulfate is shown to be a function of the tetrabutylammonium counterion concentration in the aqueous phase whether the counterions are provided by the surfactant or both the surfactant and added tetrabutylammonium bromide. The micellized surfactant dissociates 17% of its counterions to aqueous phase.     

Influence of the processing solvent on the photoactive layer nanomorphology of P3HT/PC60BM solar cells

In this article, it is demonstrated that doctor blading of thin poly‐3‐hexylthiophene/phenyl‐C₆₁‐butyric acid methyl ester (1/1) bulk‐hetero junction films from toluene leads to an improved nanocrystallinity, when compared with their unannealed chlorobenzene processed counterparts. This difference in morphology was demonstrated by solid‐state NMR and Rapid Heating Cooling Calorimetry (RHC), being useful complementary techniques to investigate the active layer morphology of photovoltaic devices. An increased PC₆₀BM nanocrystallinity is indicated by several NMR relaxation decay times (T_1C, T_1H, and T_1ρH) and confirmed by an increase of the melting enthalpy in RHC experiments. An improved solar cell performance further strengthens this conclusion. © 2011 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem, 2012     

Lipidic polyols using thiol‐ene/yne strategy for crosslinked polyurethanes

Oleic acid and α,ω‐diacid were converted into propargylic esters followed by thiol‐ene/yne coupling (TEC/TYC) functionalization in presence of mercaptoethanol. The multiradical addition on fatty esters leads to the formation of lipidic polyols (OH1 and OH2), as judged by ¹H NMR and mass spectroscopies as well as by size exclusion chromatography. The crosslinking reaction between TEC/TYC‐based polyols and 4,4′‐methylene bis(phenylisocyanate) isocyanate reactant was monitored by FTIR experiment and reaction parameters were optimized. By differential scanning calorimetry, relatively high glass transitions are measured corresponding to structure with little or without dangling chain. Moreover, the thermal stability of the resulting plant oil‐based polyurethane materials (PU1 and PU2) were found to be fully consistent with that of other lipidic PUs respecting a three‐step process. Thanks to TYC methodology, fatty α,ω‐diacid produces lipidic polyol without dangling chain and lipidic thermoset PU with relatively high T_g. © 2014 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2014 , 52, 1597–1606     

High tilt antiferroelectric esters bearing a perfluorobutanoyloxy terminal chain: the influence of lateral fluoro substituents on mesomorphic behaviour,tilt angle,and spontaneous polarization

Novel liquid crystalline esters have been synthesized with a perfluorocarbonyloxy terminal chain and lateral fluoro substituents in order to determine their influence on mesomorphic behaviour, tilt angle and spontaneous polarization. Certain locations of lateral fluoro substituents provide for very low melting points and reasonably wide temperature ranges of the antiferroelectric phase, and can generate enhanced tilt angles of up to 45°, although spontaneous polarization is always lower than for the parent system without lateral fluoro substituents. Thus, these novel materials, particularly as components in mixtures, should be useful in high tilt antiferroelectric (orthoconic) applications.     

New method and treatment technique applied to interband transition in GaAs<Subscript>1−<Emphasis Type="Italic">x</Emphasis></Subscript>P<Subscript><Emphasis Type="Italic">x</Emphasis></Subscript> ternary alloys

In this paper we presented a new method (Eigen-Coordinates (ECs)) that can be used for calculations of the critical points (CPs) energy of the interband-transition edges of the heterostructures. This new method is more accurate and complete in comparison with conventional ones and has a wide range of application for the calculation of the fitting parameters related to nontrivial functions that initially have nonlinear fitting parameters that are difficult to evaluate. The new method was applied to determine the CPs energies from the dielectric functions of the MBE grown GaAs_1−xP _x ternary alloys obtained using spectroscopic ellipsometry (SE) measurements at room temperature in the 0.5-5 eV photon energy region. The obtained results are in good agreement with the results of the other methods.     

<Emphasis Type="Italic">W</Emphasis><Superscript>+</Superscript><Emphasis Type="Italic">W</Emphasis><Superscript>+</Superscript> plus dijet production in the <Emphasis FontCategory="NonProportional">POWHEG BOX</Emphasis>

We present an implementation of the calculation of the production of W ⁺ W ⁺ plus two jets at hadron colliders, at next-to-leading order (NLO) in QCD, in the POWHEG framework, which is a method that allows the interfacing of NLO calculations to shower Monte Carlo programs. This is the first 2→4 process to be described to NLO accuracy within a shower Monte Carlo framework. The implementation was built within the POWHEG BOX package. We discuss a few technical improvements that were needed in the POWHEG BOX to deal with the computer intensive nature of the NLO calculation, and argue that further improvements are possible, so that the method can match the complexity that is reached today in NLO calculations. We have interfaced our POWHEG implementation with PYTHIA and HERWIG, and present some phenomenological results, discussing similarities and differences between the pure NLO and the POWHEG+PYTHIA calculation both for inclusive and more exclusive distributions. We have made the relevant code available at the POWHEG BOX web site.     

Strong and conditional invariance principles for samples attracted to stable laws

Summary. We prove almost sure convergence of a representation of normalized partial sum processes of a sequence of i.i.d. random variables from the domain of attraction of an α-stable law, α<2. We obtain an explicit form of the limit in terms of the LePage series representation of stable laws. One consequence of these results is a conditional invariance principle having applications to option pricing as well as to resampling by signs and permutations. Received: 11 April 1994 / In revised form: 5 November 1996     

Champs aléatoires intermittents. Partie II : champs à accroissements dissymétriques

In a previous Note (part I) we presented a family of multifractal random fields, the increments of which have a symmetrical distribution. In this second part we construct another family of intermittent fields, with asymmetric increments. To cite this article: J. Duchon, R. Robert, C. R. Acad. Sci. Paris, Ser. I 341 (2005).