Development and validation of a HPLC method for quantification of rivastigmine in rat urine and identification of a novel metabolite in urine by LC‐MS/MS

A sensitive, specific and accurate HPLC method for the quantification of rivastigmine (RSM) in rat urine was developed and validated. The method involves the simple liquid–liquid extraction of RSM and pyridostigmine as an internal standard (IS) from rat urine with tertiary methyl butyl ether. The chromatographic separation of RSM and IS was achieved with 20 mm ammonium acetate buffer (pH 6.5) and acetonitrile (65:35, v/v) delivered at flow‐rate of 1 mL/min on a Kromasil KR‐100. The method was in linear range from 50 to 5000 ng/mL. The validation was done as per FDA guidelines and the results met the acceptance criteria. The method was successfully applied for the quantification of RSM in rat urine. Besides method validation, we have identified two metabolites of RSM in urine. Both the metabolites were characterized by HPLC‐PDA and LC‐MS/MS and it was found that one metabolite is novel. Copyright © 2010 John Wiley & Sons, Ltd.     

p‐Methoxy­benz­aldehyde benzoyl­hydrazone monohydrate

The crystal structure of the title compound, C₁₅H₁₄N₂O₂·H₂O, is in the keto tautomeric form and the configuration at the azomethine C=N double bond is E. The mol­ecule is non‐planar, with a dihedral angle of 27.3 (1)° between the aromatic rings. The crystal structure is stabilized by extensive hydrogen bonding involving the water mol­ecule and hydrazone moiety.     

Asymmetric synthesis of (S)-vigabatrin and (S)-dihydrokavain via cobalt catalyzed hydrolytic kinetic resolution of epoxides

A concise route to the asymmetric synthesis of (S)-vigabatrin^® and (S)-dihydrokavain has been described using Co-catalyzed hydrolytic kinetic resolution of racemic epoxides and regiospecific opening of terminal epoxides with dimethylsulfonium methylide as the key steps.     

<Emphasis Type="Italic">C</Emphasis>-field Cosmological Models with Variable <Emphasis Type="Italic">G</Emphasis> in FRW Space–Time

C-field cosmological models based on Hoyle-Narlikar theory with variable gravitational constant G in the frame work of FRW (Friedmann-Robertson-Walker) space–time for positive and negative curvatures are investigated. To get the deterministic solutions in terms of cosmic time t, we have assumed G=R ⁿ and discussed for n=−1, −2, R being scalar factor. In both the cases, creation field C increases with time, the gravitational constant G and matter density (ρ) decrease with time in the model (21). In the model (41) G decreases with time and matter density (ρ) is constant. The other physical aspects of the models are also discussed.     

Magnesium ion conducting, room temperature molten electrolytes

Room temperature, magnesium ion conducting binary molten electrolyte consisting of acetamide and magnesium perchlorate has been prepared and characterized. The molten liquid is very stable and shows high ionic conductivity, of the order of several mS cm⁻¹ at 25 °C with other favourable physicochemical properties. Vibrational spectroscopic studies reveal that the free ion concentration is higher than that of ion pairs and aggregates in the melt. The electrochemical reversibility of magnesium deposition and dissolution is demonstrated using voltammetry and impedance studies. Preliminary studies on rechargeable batteries assembled using γ-MnO₂ and Mg metal as the electrodes together with the molten electrolyte show high discharge capacity.     

Syntheses,crystal structures and spectroscopic properties of the novel complexes [M(MOBPT)*2 (H2O)2](ClO4)2 · 4H2O (M = CoII and NiII)

The novel cobalt(II) and nickel(II) complexes with 4-(p-methoxyphenyl)-3,5-bis(pyridin-2-yl)-1,2,4-triazole (MOBPT) have been synthesized and their molecular structures determined by X-ray analysis, i.r. and by ESI-MS spectroscopy. The metal atom is in a distorted octahedral environment. Two bidentate chelating ligands (MOBPT) coordinate to the metal center equatorially and two water molecules coordinate axially. Each MOBPT entity coordinates via one triazole nitrogen and one pyridine nitrogen atom. Magnetic measurements show that the complexes are high-spin species in the 75–300 K range.