Biomolecular Fishing for Calixarene Partners by a Chemoproteomic Approach

MS‐based chemical‐proteomics technology is introduced herein as a third general strategy to study the biomolecular recognition properties of given calixarene derivatives. In particular, we demonstrate that a simply designed calix[4]arene derivative 1 a bearing acetamido groups at the exo rim (pAC), when linked to a solid support, is able to fish out a specific protein (PDI protein) from a crude extract of HeLa cells. Western blot and surface plasmon resonance studies confirmed the direct interaction between PDI and the linker‐free pAC derivative 1 b with considerable affinity, and in vitro tests showed its inhibition of PDI chaperone activity. In accordance with the role of PDI in a variety of human cancers, biological tests showed that pAC 1 b was cytotoxic and cytostatic toward CAL‐27 and PC‐3 cancer cell lines in vitro. Docking studies showed that H bonds and hydrophobic interactions contribute to the stabilization of the PDI/pAC complex.     

Inclusive and exclusive measurements of high energy γ-rays in the 101 MeV <Superscript>19</Superscript>F +<Superscript>181</Superscript>Ta fusion reaction

The high energy γ-rays from the fusion-evaporation reaction 101 MeV ¹⁹F +¹⁸¹Ta have been measured in coincidence with different γ-ray fold windows or discrete γ-rays in final residual nuclei. The line-shape analysis of the high energy γ-ray spectra confirms the large deformation of the ²⁰⁰Pb nuclei at high angular momenta. Coincidences with discrete γ-transitions evidence a strong correlation between γ-rays in the Giant Dipole Resonance region (E_γ≥ 8 MeV) and final evaporation residues. Received: 14 September 1999 / Revised version: 17 December 1999     

Study of the Mo-Ba partition in 252Cf spontaneous fission

Measurements of fission fragment yields and neutron multiplicities have been carried out for the Mo-Ba fragment pairs in the spontaneous fission of ²⁵²Cf, using the γ-ray spectroscopy technique to analyze γ-γ-γ coincidence data. Prompt γ -ray multiplicities were also measured as a function of the number of neutrons emitted in the fission process leading to the Mo-Ba partition. We do not observe the enhancement in the yields of events with high neutron emission multiplicity (ν_n > 7) that has been associated to a second fission mode leading to the production of hyperdeformed Ba fragments, as reported in some earlier studies. The average γ-ray multiplicity is found to be rather weakly dependent on the number of neutrons emitted in the fission process. Received: 21 July 1999 / Revised version: 19 November 1999     

Fragment dependence of high energy γ-ray emission in the spontaneous fission of 252Cf

The high energy γ-ray emission accompanying the spontaneous fission of ²⁵²Cf has been measured in coincidence with individual fission fragments selected by discrete γ-ray transitions. The enhancement of the γ-ray emission probability in the energy range E_γ= 3–8 MeV has been observed for the fission fragments in the region of nearly symmetric mass splitting, confirming results reported in previous investigations. The γ-γ coincidence technique employed in the present work clearly demonstrate that the major contribution to this enhancement is caused by the fission channels where one fragment is near to the N= 82 or Z= 50 shell closures. The high energy γ-ray emission probability does not show any significant dependence on the number of neutrons emitted in the fission process, supporting the hypothesis that high energy γ-rays are mainly emitted from the fragments after the neutron evaporation. Received: 22 December 1998     

Feasibility of a Nano‐Carbon Black Paste Electrode for Simultaneous Voltammetric Determination of Antioxidants in Food Samples and Biodiesel in the Presence of Surfactant

In this work, a simple and low‐cost method was developed for the simultaneous determination of the antioxidants tert‐butylhydroquinone (TBHQ) and butylated hydroxyanisole (BHA) in the presence of the cationic surfactant CPB by square wave voltammetry (SWV) technique using a carbon black paste electrode (CBPE). The performance of the method was investigated by varying parameters such as pH, electrolyte, and type and concentration of surfactant. Under the optimum conditions of 0.2 mol L^?1 phosphate buffer (pH 7.0), 600.0 μmol L^?1 of cetylpyridinium bromide surfactant and SWV operating parameters optimized through the Doehlert matrix, the method presented low limits of quantification for TBHQ and BHA (0.23 and 0.26 μmol L^?1, respectively) and high precision in successive measurements. The proposed method was applied in mayonnaise, margarine and biodiesel and the accuracy of method was checked by the HPLC technique.     

Cylindrically symmetric relativistic fluids: a study based on structure scalars

Applying the 1 + 3 formalism we write down the full set of equations governing the structure and the evolution of self-gravitating cylindrically symmetric dissipative fluids with anisotropic stresses, in terms of scalar quantities obtained from the orthogonal splitting of the Riemann tensor (structure scalars), in the context of general relativity. These scalars which have been shown previously (in the spherically symmetric case) to be related to fundamental properties of the fluid distribution, such as: energy density, energy density inhomogeneity, local anisotropy of pressure, dissipative flux, active gravitational mass etc, are shown here to play also a very important role in the dynamics of cylindrically symmetric fluids. It is also shown that in the static case, all possible solutions to Einstein equations may be expressed explicitly through three of these scalars.     

Laboratory tests of low density astrophysical nuclear equations of state

Clustering in low density nuclear matter has been investigated using the NIMROD multidetector at Texas A&M University. Thermal coalescence modes were employed to extract densities, ρ, and temperatures, T, for evolving systems formed in collisions of 47A MeV (40)Ar+(112)Sn, (124)Sn and (64)Zn+(112)Sn, (124)Sn. The yields of d, t, (3)He, and (4)He have been determined at ρ=0.002 to 0.03 nucleons/fm(3) and T=5 to 11 MeV. The experimentally derived equilibrium constants for α particle production are compared with those predicted by a number of astrophysical equations of state. The data provide important new constraints on the model calculations.     

Dynamics of superheavy system in 86Kr + 208Pb reaction

The dynamics of the excited superheavy system with Z = 118 in the reaction ⁸⁶Kr + Pb at E _Kr = 600 MeV has been investigated. The mass and kinetic energy of binary fragments were measured by the time-of-flight method. Double differential distributions of neutrons and α particles were measured in coincidence with fragments. Neutron and α-particle probes were used for determination of the fragmentation time scale. Evidence of the neck fragmentation was obtained from analysis of double differential α spectra. Properties of the α-particle neck fragmentation component are close to those known from the ternary fission of actinide nuclei, but the multiplicity is much larger. The total kinetic energy distribution of fragments tagged by neutrons or α particles shifts towards lower energies. Fragment yields in the symmetric region increase substantially when fragments are tagged by α particles.     

Light particle accompanied quasifission in superheavy composite systems

The dynamics of the excited superheavy system with Z = 118 in the reaction ⁸⁶Kr + ²⁰⁸Pb at E _Kr = 460, 500, and 600 MeV has been investigated. The mass and kinetic energy of binary fragments were measured by the time-of-flight method. Double-differential distributions of protons and α particles were measured in coincidence with fragments. The proton spectra can be described considering only evaporation from fragments. Evidence of the neck fragmentation was obtained from analysis of double-differential α spectra. Properties of the α-particle neck fragmentation component are close to those known from the ternary fission of actinide nuclei, but the multiplicity is much larger than can be expected from extrapolation of the ternary fission data. The text was submitted by the authors in English.