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101.
102.
近些年来,随着国内外尖端科技快速发展,温度测量无论是对于国防建设领域还是对于工业制造领域都有着极为重要的指导意义和研究价值。尤其在瞬态超高温测量方面,测温精度要求更为严苛。测温方法多种多样,多光谱法由于其精度较高且适用性强,被国内外专家广泛运用。基于多光谱测温法,提出一种新的能够同时高精度测量目标的瞬态激发温度和辐射温度的方法。该方法通过查找可信度更高的目标物理特性数据以及更为精确的多光谱直线拟合方法,精准计算得到目标激发温度。通过建立更加准确的数学模型和算法,减小光谱发射率对整个测温过程的影响实现高精度的辐射温度测量。通过相关测温实验表明,系统测温精度达到3%。 相似文献
103.
Xi Nian FANG Jian Hua WANG 《数学学报(英文版)》2006,22(6):1819-1824
In this paper, we study the extension of isometries between the unit spheres of normed space E and C(Ω). We obtain that any surjective isometry between the unit spheres of normed space E and C(Ω) can be extended to be a linear isometry on the whole space E and give an affirmative answer to the corresponding Tingley's problem (where Ω be a compact metric space). 相似文献
104.
Screening of High-Affinity scFvs From a Ribosome Displayed Library Using BIAcore Biosensor 总被引:2,自引:0,他引:2
An experimental protocol was developed to screen high-affinity single-chain Fv antibody fragments (scFvs) from a Xanthomonas axonopodis pv. citri (Xac) immunized ribosome display library using BIAcore biosensor. The screening methods involved immobilizing antigen [lipopolysaccharides
(LPS) of Xac] on sensor chip HPA and then unpurified expression products of scFvs flowing over the immobilized sensor chip.
The affinity-improved scFvs were selected based on dissociation rate constants (k
d). Thirty-five enzyme-linked immunosorbent assay-positive scFvs were analyzed by BIAcore, and three of those (scFv A1, B2,
and C5) with lower k
d were screened. To demonstrate the accuracy of the screening method, the three scFvs were expressed in Escherichia coli HB2151 and purified. The purified scFvs were subsequently further identified according to association rate and affinity constants.
The results showed that the three scFvs (A1, B2, and C5) had high affinity for LPS of Xac (3.51 × 10−11, 1.13 × 10−10, 5.06 × 10−10 M, respectively). Furthermore, the scFv B2 was highly specific for LPS of Xac and had no any cross-reactions with bovine
serum albumin and LPS from Xac-related bacteria. This provided evidence that the information from the BIAcore screening assay
could be accurate. 相似文献
105.
A poly(thionine) thin film modified electrode was successfully assembled on the surface of the glassy carbon electrode by means of electrochemical polymerization, which was carried out with cyclic voltammetric sweeping in the potential range 0 to +1.4 V (vs. Ag/AgCl) in perchloric acid solution containing 0.1 mmol L?1 thionine. The film modified electrode exhibited a couple of well-defined redox peaks, and the redox peaks decreased correspondingly without a shift of the peak potential after the addition of heparin. The conditions of the binding reaction and the electrochemical detection were optimized. Under the optimum conditions the decrease of the peak current was proportional to the concentration of heparin in the range 4.0 to 22.0 μg mL?1 and the detection limit was 0.28 μg mL?1. The relative standard deviation (RSD) for five parallel determinations of 10.0 μg mL?1 heparin was 0.93%. The effects of potentially interfering species were investigated and the method was successfully applied to the determination of heparin in a pharmaceutical formulation. 相似文献
106.
Nian Sun Jiangxiao Sun Elena N. Kitova John S. Klassen 《Journal of the American Society for Mass Spectrometry》2009,20(7):1242-1250
The application of the reporter molecule (Mrep) method for identifying nonspecific complexes in the ES-MS analysis of protein-ligand and DNA-ligand interactions in vitro
is described. To test the reliability of the method, it was applied to the ES-MS analysis of protein-carbohydrate complexes
originating from specific interactions in solution and from nonspecific interactions in the ES process. These control experiments
confirm the basic assumptions underlying the Mrep method, namely that nonspecific ligand binding is a random process, and that the ES droplet histories for specific and nonspecific
complexes are distinct. The application of the Mrep method to the ES-MS analysis of the sequential binding of the ethidium cation, a DNA intercalator, to single and double strand
oligodeoxynucleotides is also described, and highlights the general utility of the method. 相似文献
107.
Arnaud Guillin Christian Léonard Liming Wu Nian Yao 《Probability Theory and Related Fields》2009,144(3-4):669-695
In this paper, one investigates the transportation-information T c I inequalities: α(T c (ν, μ)) ≤ I (ν|μ) for all probability measures ν on a metric space ${(\mathcal{X}, d)}$ , where μ is a given probability measure, T c (ν, μ) is the transportation cost from ν to μ with respect to the cost function c(x, y) on ${\mathcal{X}^2}$ , I(ν|μ) is the Fisher–Donsker–Varadhan information of ν with respect to μ and α : [0, ∞) → [0, ∞] is a left continuous increasing function. Using large deviation techniques, it is shown that T c I is equivalent to some concentration inequality for the occupation measure of a μ-reversible ergodic Markov process related to I(·|μ). The tensorization property of T c I and comparisons of T c I with Poincaré and log-Sobolev inequalities are investigated. Several easy-to-check sufficient conditions are provided for special important cases of T c I and several examples are worked out. 相似文献
108.
A molecularly imprinted polymer (MIP) capable of detecting bilirubin was successfully synthesized. Bilirubin template was imprinted in poly(methacrylic acid-co-ethylene glycol dimethylacrylate) [poly(MAA-co-EGDMA)]. MAA and EGDMA were used as the monomer and the cross-linker, respectively. The optimal solvent conditions to maintain its stability were discussed. Solvent system based on ethylenediamine tetraacetic acid (EDTA) and ascorbic acid was compared with respect to the stability of bilirubin. pH and bilirubin concentration were both investigated for the bilirubin stability. Blue light as well as aeration was applied to inspect the regarding effects. The cross-linking effect was further confirmed by the thermogravimetric analysis (TGA). The effect of salts, such as NaCl and KCl on the binding capacity of the molecularly imprinted polymer was also discussed. Further, the rat serum and bile samples were applied and the binding of the MIPs for bilirubin was thus confirmed. 相似文献
109.
以Ce(NO3)3.5H2O,Y(NO3)3.5H2O和A l(NO3)3.9H2O为原料,NH4HCO3为沉淀剂,采用醇水溶剂共沉淀法在弱还原气氛下1100℃煅烧2 h合成纯相1 at%Ce∶YAG纳米粉体,经1800℃真空烧结10 h后得到了Ce∶YAG透明陶瓷。通过XRD、TEM、SEM和荧光光谱仪对粉体和陶瓷进行了表征。结果表明:所制得的Ce∶YAG纳米粉体散均匀、团聚程度轻、结晶良好,平均晶粒尺寸约35 nm。Ce∶YAG透明陶瓷和纳米粉体的发射峰都在530 nm附近,但透明陶瓷的发射强度明显比纳米粉体强,且发射峰向长波方向移动。 相似文献
110.
Solid-state resonant tunneling thermoelectric refrigeration in the cylindrical double-barrier nanostructure 下载免费PDF全文
A solid-state thermoelectric refrigerator with a cylindrical InP/InAs/InP double-barrier heterostructure is proposed.Based on the ballistic electron transport and the asymmetrical transmission, we derive the expressions of the performance parameters of this refrigerator. The cooling rate rather than the coefficient of performance is affected by the area of the inner cylinder. Then through the numerical simulation, a triangular cooling rate region is found with respect to the chemical potential and bias voltage; further, that it is because of the small full width at half maximum of the transmission resonance and the linear relationship between the energy position of resonance and the bias voltage. These tunable results might supply some guide to the cooling in tiny components or devices. 相似文献