NMR study of catechol and some catecholamines

The proton magnetic resonance spectra of catechol, epinephrine, dopamine, synephrine, tyramine and amphetamine have been analysed by computer simulation. The chemical shifts and coupling constants are discussed, with their molecular implications. Convenient parameters are introduced for the additivity of substituent effects on chemical shifts of aromatic protons. The additivity rule of coupling constants is extended to these compounds.     

Soft π−p and pp elastic scattering in the energy range 30 to 345 GeV

Differential cross sections for π^?p and pp elastic scattering have been measured at incident momenta ranging from 30 to 345 GeV and in the t range 0.002 (GeV/c)² ? |t| ? 0.04 (GeV/c)². From the analysis of the data, the ratio ? (t = 0) of the real to the imaginary parts of the forward scattering amplitude was determined together with the logarithmic slope b of the diffraction cone.The results on the real parts confirm the validity of the forward dispersion relations at high energies. Using the dispersion relations, it was shown that the experimental data on ?_{π?p(t = 0)} require a continuous rise of the total πp cross sections, at least up to the energy of 2000 GeV, thus revealing a close similarity in high-energy behaviour of πp and pp interactions.The results on the slope parameters from this experiment together with the analysis of the available world data demonstrate that the existing experimental data are consistent with the hypothesis of a universal shrinkage of the hadronic diffraction cone at high energies. The value of the asymptotic shrinkage parameter α_p^′ was found to be independent of the kind of the incident hadron and of the momentum transfer in the t range |t| ? 0.2 (GeV/c)² : 2α_{p^′ = ± 3 (GeV/c)^?2}.     

High resolution infrared spectra and structure of borine carbonyl: The analysis of the ν2 bands of 11BD3CO and 10BD3CO

The high resolution infrared spectra of ¹⁰BD₃CO and ¹¹BD₃CO were obtained in the region of the ν₂ fundamentals. The K = 0 subbands of the ν₂ and ν₂ + ν₈ ? ν₈ bands were assigned. The K structure is unresolvable with a spectral slit width of 0.03 cm^?1. A series, almost as strong as the main series, has been identified in the spectrum of the ¹¹B molecule but has not been assigned. It is shown that the four structural parameters of borine carbonyl cannot be accurately determined using only the B₀ rotational constants of four isotopically substituted molecules.     

Metalloproteinase inhibitors for the disintegrin-like metalloproteinases ADAM10 and ADAM17 that differentially block constitutive and phorbol ester-inducible shedding of cell surface molecules

The transmembrane metzinkin-proteases of the ADAM (a disintegrin and a metalloproteinase)-family ADAM10 and ADAM 17 are both implicated in the ectodomain shedding of various cell surface molecules including the IL6-receptor and the transmembrane chemokines CX3CL1 and CXCL16. These molecules are constitutively released from cultured cells, a process that can be rapidly enhanced by cell stimulation with phorbol esters such as PMA. Recent research supports the view that the constitutive cleavage predominantly involves ADAM10 while the inducible one is mediated to a large extent by ADAM17. We here describe the discovery of hydroxamate compounds with different potency against ADAM10 and ADAM17 and different ability to block constitutive and inducible cleavage of IL6R, CX3CL1 and CXCL16 by the two proteases. By screening a number of hydroxamate inhibitors for the inhibition of recombinant metalloproteinases, a compound was found inhibiting ADAM10 with more than 100-fold higher potency than ADAM17, which may be explained by an improved fit of the compound to the S1' specificity pocket of ADAM10 as compared to that of ADAM17. In cell-based cleavage experiments this compound (GI254023X) potently blocked the constitutive release of IL6R, CX3CL1 and CXCL16, which was in line with the reported involvement of ADAM10 but not ADAM17 in this process. By contrast, the compound did not affect the PMA-induced shedding, which was only blocked by GW280264X, a potent inhibitor of ADAM17. As expected, GI254023X did not further decrease the residual release of CX3CL1 and CXCL16 in ADAM10-deficient cells verifying that the compound's effect on the constitutive shedding of these molecules was exclusively due to the inhibition of ADAM10. Thus, GI254023X may by of use as a preferential inhibitor of constitutive shedding events without effecting the inducible shedding in response to agonists acting similar to PMA.