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201.
202.
Metabolic monitoring of the electrically stimulated single heart cell within a microfluidic platform 总被引:2,自引:0,他引:2
A device based on five individually addressable microelectrodes, fully integrated within a microfluidic system, has been fabricated to enable the real-time measurement of ionic and metabolic fluxes from electrically active, beating single heart cells. The electrode array comprised one pair of pacing microelectrodes, used for field-stimulation of the cell, and three other microelectrodes, configured as an electrochemical lactate microbiosensor, that were used to measure the amounts of lactate produced by the heart cell. The device also allowed simultaneous in-situ microscopy, enabling optical measurements of cell contractility and fluorescence measurements of extracellular pH and cellular Ca2+. Initial experiments aimed to create a metabolic profile of the beating heart cell, and results show well defined excitation-contraction (EC) coupling at different rates. Ca2+ transients and extracellular pH measurements were obtained from continually paced single myocytes, both as a function of the rate of cell contraction. Finally, the relative amounts of intra- and extra-cellular lactate produced during field stimulation were determined, using cell electroporation where necessary. 相似文献
203.
Godfrey JD Fox RT Buono FG Gougoutas JZ Malley MF 《The Journal of organic chemistry》2006,71(22):8647-8650
Conversion of an alpha,alpha-dichloroester to the corresponding alpha-keto acid was unexpectedly complicated by a novel 1,4-homofragmentation. Investigation of the kinetics of this reaction revealed a mechanism involving an alpha-lactone intermediate, which can lead to both the desired alpha-keto acid and the 1,4-homofragmentation, with the product distribution being dependent upon reaction conditions. This information allowed development of a process that affords the alpha-keto acid exclusively and should be generally applicable to the preparation of alpha-keto acids from alpha,alpha-dichloroesters or acids. 相似文献
204.
We investigated the transmission of Dirac electrons through a potential barrier in the presence of circularly polarized light. An anomalous photon-assisted enhanced transmission is predicted and explained. It is demonstrated that the perfect transmission for nearly head-on collision in infinite graphene is suppressed in gapped dressed states of electrons, which is further accompanied by a shift of peaks as a function of the incident angle away from head-on collision. In addition, the perfect transmission is partially suppressed by a photon-induced gap in illuminated graphene. After the effect of rough edges of the potential barrier or impurity scattering is included, the perfect transmission with no potential barrier becomes completely suppressed and the energy range for the photon-assisted transmission is reduced at the same time. 相似文献
205.
This study describes the vocal repertoire of the Guyanan golden rocket frog, Anomaloglossus beebei, a bromeliad specialist with biparental care. Using multivariate analyses of nine call properties, as well as the occurrence of nonlinear phenomena, three signal types were distinguished-advertisement, courtship, and aggressive calls. Although all three call types were composed of a short series of rapidly repeated pulses, advertisement calls were produced at higher amplitudes and had longer pulse durations than both courtship calls and aggressive calls. Courtship calls exhibited lower dominant frequencies than both advertisement and aggressive calls, which had similar dominant frequencies. Aggressive calls had more pulses per call, had longer intervals between calls, and occasionally contained one or two introductory pulses preceding the pulsed call. Several acoustic properties predicted aspects of the signaler's body size and condition. Our study demonstrates the reliability of human observers to differentiate the multiple call types of A. beebei based on hearing calls and observing the social context in which they are produced under field conditions. 相似文献
206.
In this article we present a fourth‐order finite difference scheme, for a system of two‐dimensional, second‐order, nonlinear elliptic partial differential equations with mixed spatial derivative terms, using 13‐point stencils with a uniform mesh size h on a square region R subject to Dirichlet boundary conditions. The scheme of order h4 is derived using the local solution of the system on a single stencil. The resulting system of algebraic equations can be solved by iterative methods. The difference scheme can be easily modified to obtain formulae for grid points near the boundary. Computational results are given to demonstrate the performance of the scheme on some problems including Navier‐Stokes equations. © 2001 John Wiley & Sons, Inc. Numer Methods Partial Differential Eq 17: 43–53, 2001 相似文献