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541.
Theodoridis G Lasáková M Skeríková V Tegou A Giantsiou N Jandera P 《Journal of separation science》2006,29(15):2310-2321
As shown in the past years, SPE based on molecularly imprinted polymers (MIPs) may provide significant enhancement of selectivity in sample preparation and analyte preconcentration. The objective of this work was the fabrication of MIPs for the specific adsorption of rutin and quercetin. The two flavonoids were used as the template molecules for the preparation of MIP phases in a self-assembly (noncovalent) approach. The produced MIPs were validated with regard to the imprinting efficiency as media for LC and SPE. The retention behavior of several flavonoid compounds was studied using as stationary phases imprinted, control nonimprinted polymers, and commercial silica-based materials. MIPs were applied as materials for the selective SPE and preconcentration of the flavonoids from white and red wine, orange juice, and tea. The collected fractions were analyzed by high-pressure LC. MIP-SPE facilitated specific analyte isolation and effective sample clean-up. The results show that molecularly imprinted SPE can be a useful tool for the simple, selective, and cost-effective pretreatment of samples containing natural antioxidants. 相似文献
542.
Zacharis CK Tempels FW Theodoridis GA Voulgaropoulos AN Underberg WJ Somsen GW de Jong GJ 《Journal of chromatography. A》2006,1132(1-2):297-303
The on-line coupling of sequential injection analysis (SIA) and capillary electrophoresis (CE) via an in-line injection valve is presented. The SIA system is used for automated derivatization of amino acids and peptides. Dichlorotriazinylaminofluorescein serves as the derivatization agent, thus enabling sensitive laser-induced fluorescence detection of the derivatized analytes. The SIA procedure includes the following steps: (a) introduction of reagent and sample zones in a holding coil, (b) sample and reagent mixing in a reaction coil, (c) stop-flow step for increase of the reaction time, and (d) delivery of derivatized sample into the loop of the micro-valve interface. A small portion of the analyte zone is introduced electrokinetically in the separation capillary via the valve interface and CE analysis is performed. Factors affecting the CE separation, such as pH, the borate and sodium dodecyl sulphate concentration of the background electrolyte have been optimized. The derivatization conditions have been studied to obtain a high reaction yield in a relative short time. The transfer of a part of the reaction plug into the loop of the valve interface has been optimized. Using des-Tyr(1)-[Met]-enkephalinamide as test compound, it is demonstrated that after automated derivatization, on-line electrophoretic analysis could be achieved. Glycine has been selected as the internal standard in order to correct for variations in reaction time and filling of the injection loop. For the enkephalin, good reproducibility (RSD<4.5% calculated by the ratio of the peak areas) and linearity (0.5-5 microg mL(-1), R(2)>or=0.994) are obtained with a detection limit of 30 ng mL(-1) (S/N=3). 相似文献
543.
Chiu ME Kyriakou G Williams FJ Watson DJ Tikhov MS Lambert RM 《Chemical communications (Cambridge, England)》2006,(12):1283-1285
Sulfur adatoms strongly activate the otherwise inert Cu(111) surface towards chemoselective hydrogenation of crotonaldehyde by electronically perturbing and strongly tilting the reactant. 相似文献
544.
The decatungstate W10O32(4-) homogeneous photocatalyzed oxygenation of tetrasubstituted alkenes has been mechanistically studied. In all cases, allylic hydroperoxides are the major products. The primary inter- and intramolecular as well as the remote delta-secondary deuterium kinetic isotope effects for the photooxidation of the 2,3-dimethyl-2-butene and 1,1,1-trideuterio-7-methyl-2-(trideuteriomethyl)octa-2,6-diene along with product analysis suggest a hydrogen abstraction in the rate-determining step. For comparison, singlet oxygen photosensitized oxidations of the above substrates were also studied. 相似文献
545.
546.
Panagiota Zakidou Fotini Plati Anthia Matsakidou Evdoxia-Maria Varka Georgios Blekas Adamantini Paraskevopoulou 《Molecules (Basel, Switzerland)》2021,26(15)
In this study, the aroma profile of 10 single origin Arabica coffees originating from eight different growing locations, from Central America to Indonesia, was analyzed using Headspace SPME-GC-MS as the analytical method. Their roasting was performed under temperature–time conditions, customized for each sample to reach specific sensory brew characteristics in an attempt to underline the customization of roast profiles and implementation of separate roastings followed by subsequent blending as a means to tailor cup quality. A total of 138 volatile compounds were identified in all coffee samples, mainly furan (~24–41%) and pyrazine (~25–39%) derivatives, many of which are recognized as coffee key odorants, while the main formation mechanism was the Maillard reaction. Volatile compounds’ composition data were also chemometrically processed using the HCA Heatmap, PCA and HCA aiming to explore if they meet the expected aroma quality attributes and if they can be an indicator of coffee origin. The desired brew characteristics of the samples were satisfactorily captured from the volatile compounds formed, contributing to the aroma potential of each sample. Furthermore, the volatile compounds presented a strong variation with the applied roasting conditions, meaning lighter roasted samples were efficiently differentiated from darker roasted samples, while roasting degree exceeded the geographical origin of the coffee. The coffee samples were distinguished into two groups, with the first two PCs accounting for 73.66% of the total variation, attributed mainly to the presence of higher quantities of furans and pyrazines, as well as to other chemical classes (e.g., dihydrofuranone and phenol derivatives), while HCA confirmed the above results rendering roasting conditions as the underlying criterion for differentiation. 相似文献
547.
Adamantios Krokos Olga Deda Christina Virgiliou Helen Gika Nikolaos Raikos Eleni Aggelidou Aristeidis Kritis Georgios Theodoridis 《Molecules (Basel, Switzerland)》2021,26(15)
Cocaine toxicity has been a subject of study because cocaine is one of the most common and potent drugs of abuse. In the current study the effect of cocaine on human liver cancer cell line (HepG2) was assessed. Cocaine toxicity (IC50) on HepG2 cells was experimentally calculated using an XTT assay at 2.428 mM. The metabolic profile of HepG2 cells was further evaluated to investigate the cytotoxic activity of cocaine at 2 mM at three different time points. Cell medium and intracellular material samples were analyzed with a validated HILIC-MS/MS method for targeted metabolomics on an ACQUITY Amide column in gradient mode with detection on a triple quadrupole mass spectrometer in multiple reaction monitoring. About 106 hydrophilic metabolites from different metabolic pathways were monitored. Multivariate analysis clearly separated the studied groups (cocaine-treated and control samples) and revealed potential biomarkers in the extracellular and intracellular samples. A predominant effect of cocaine administration on alanine, aspartate, and glutamate metabolic pathway was observed. Moreover, taurine and hypotaurine metabolism were found to be affected in cocaine-treated cells. Targeted metabolomics managed to reveal metabolic changes upon cocaine administration, however deciphering the exact cocaine cytotoxic mechanism is still challenging. 相似文献
548.
Philip Murgatroyd Bart Craenen Georgios Theodoropoulos Vincent Gaffney John Haldon 《Computational & Mathematical Organization Theory》2012,18(4):488-506
Although historical studies are frequently perceived as clear narratives defined by a series of fixed events; in reality, even where critical historical events may be identified, historic documentation frequently lacks corroborative detail to support verifiable interpretation. Consequently, interpretation rarely rises above the level of unproven assertion and is rarely tested against a range of evidence. Agent-based simulation can provide an opportunity to break these cycles of academic claim and counter-claim. This paper discusses the development of an agent-based simulation designed to investigate medieval military logistics so that new evidence may be generated to supplement existing historical analysis. It uses as a use-case the Byzantine army’s march to the battle of Manzikert (AD 1071), a key event in medieval history. The paper focuses primarily on the design of the agents and the environment they interact with, as well as how the agent-based simulation as a whole can be used to generate new parameters with which historical evidence can be situated. 相似文献
549.
Numerical experiments on the efficiency of local grid refinement based on truncation error estimates
Alexandros Syrakos Georgios Efthimiou John G. Bartzis Apostolos Goulas 《Journal of computational physics》2012,231(20):6725-6753
Local grid refinement aims to optimise the relationship between accuracy of the results and number of grid nodes. In the context of the finite volume method no single local refinement criterion has been globally established as optimum for the selection of the control volumes to subdivide, since it is not easy to associate the discretisation error with an easily computable quantity in each control volume. Often the grid refinement criterion is based on an estimate of the truncation error in each control volume, because the truncation error is a natural measure of the discrepancy between the algebraic finite-volume equations and the original differential equations. However, it is not a straightforward task to associate the truncation error with the optimum grid density because of the complexity of the relationship between truncation and discretisation errors. In the present work several criteria based on a truncation error estimate are tested and compared on a regularised lid-driven cavity case at various Reynolds numbers. It is shown that criteria where the truncation error is weighted by the volume of the grid cells perform better than using just the truncation error as the criterion. Also it is observed that the efficiency of local refinement increases with the Reynolds number. The truncation error is estimated by restricting the solution to a coarser grid and applying the coarse grid discrete operator. The complication that high truncation error develops at grid level interfaces is also investigated and several treatments are tested. 相似文献
550.
In the simulation of shell problems in which plastic stresses in thickness direction are developed, the necessity of a more accurate integration through thickness using more integration points e.g. Gauss points becomes obvious, often increasing respectively the computational time and effort. A new algorithm is proposed for the automatic adaptation of the integration formula in thickness direction during the computation. Simple engineering criteria are found to define the time-point and the regions to increase the number of integration points. (© 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim) 相似文献