Semi-realistic shell model studies in the lead region. II. 211Bi

The semi-realistic approach described earlier is applied to a calculation of the ²⁰⁸Pb ± 3 nucleus ²¹¹Bi. It is shown that the anomalously low lying $\text{7}\text{2}{}^{\mathrm{?}}$ first excited state arises from the particularly strong coupling between the $\text{f}{}_{\mathrm{<mtext>7</mtext><mtext>2</mtext>}}$ single-proton state and the $\text{7}\text{2}{}^{\mathrm{?}}$ member of the $\text{i}{}_{\mathrm{<mtext>13</mtext><mtext>2</mtext>}}$ ? ²⁰⁸Pb(3^?) core multiplet.     

A validated positive chemical ionization GC/MS method for the identification and quantification of amphetamine, opiates, cocaine, and metabolites in human postmortem brain

A sensitive and specific method for the simultaneous detection and quantification of amphetamine, opiates, and cocaine and metabolites in human postmortem brain was developed and validated. Analytes of interest included amphetamine, morphine, codeine, 6-acetylmorphine, cocaine, benzoylecgonine, ecgonine methyl ester, ecgonine ethyl ester, cocaethylene, and anhydroecgonine methyl ester. The method employed ultrasonic homogenization of brain tissue in pH 4.0 sodium acetate buffer and solid phase extraction. Extracts were derivatized with N-methyl-N-(tert-butyldimethylsilyl) trifluoroacetamide and N,O-bis(trimethylsilyl) trifluoroacetamide. Separation and quantification were accomplished on a bench-top positive chemical ionization capillary gas chromatograph/mass spectrometer with selected ion monitoring. Eight deuterated analogs were used as internal standards. Limits of quantification were 50 ng/g of brain. Calibration curves were linear to 1000 ng/g for anhydroecgonine methyl ester and 6-acetylmorphine, and to 2000 ng/g for all other analytes. Accuracy across the linear range of the assay ranged from 90.2 to 112.2%, and precision, as percent relative standard deviation, was less than 16.6%. Quantification of drug concentrations in brain is a useful research tool in neurobiology and in forensic and postmortem toxicology, identifying the type, relative magnitude, and recency of abused drug exposure. This method will be employed to quantify drug concentrations in human postmortem brain in support of basic and clinical research on the physiologic, biochemical, and behavioral effects of drugs in humans.     

Analytic density-functional self-consistent-field theory of diblock copolymers near patterned surfaces

Analytical solutions are derived for the density profiles and the free energies of compressible diblock copolymer melts (or incompressible copolymer solutions) near patterned surfaces. The density-functional self-consistent-field theory is employed along with a Gaussian chain model for bonding constraints and a random mixing approximation for nonbonded interactions. An analytical solution is rendered possible by expanding the chain distribution function around an inhomogeneous reference state with a nontrivial analytical solution, by retaining the linear terms, and by requiring consistency with the homopolymer limit. The density profiles are determined by both real and complex roots of a sixth-degree polynomial that may easily be obtained by solving a generalized eigenvalue problem. This analytical formulation enables one to efficiently explore the large nine-dimensional parameter space and can serve as a first approximation to computationally intensive studies with more detailed models. Illustrative computations are provided for uniform and patterned surfaces above the order-disorder transition. The results are consistent with the previous self-consistent-field calculations in that lamellar ordering appears near the surface above the order-disorder transition and the lamella order perpendicular or parallel to the surface depending on the commensurability between the periods of the surface pattern and the density oscillations.     

Investigation of the metabolism of substance P at the blood-brain barrier using capillary electrophoresis with laser-induced fluorescence detection.

Substance P (SP) metabolism was investigated upon exposure to a monolayer of bovine brain microvessel endothelial cells (BBMECs), a cell culture model of the blood-brain barrier. SP was incubated with the BBMECs and its metabolism was followed as a function of time over a 5-h period. The resulting samples were derivatized with naphthalene-2,3-dicarboxaldehyde (NDA)/cyanide, separated, and detected using cyclodextrin-modified electrokinetic chromatography with laser-induced fluorescence detection (CDMEKC-LIF). Upon exposure to the BBMEC monolayer, SP rapidly degraded to produce the N-terminal (1-9), (1-4) and (1-7) and C-terminal (2-11) and (3-11) fragments. These results were compared with those in an earlier report from our laboratory, where SP metabolism was investigated in vivo by microdialysis sampling in rat striatum.     

AN UPPER BOUND ON RANDOM BUFFETING FORCES CAUSED BY TWO-PHASE FLOWS ACROSS TUBES

This paper investigates the random buffeting excitation forces that apply to tubes in two-phase cross-flows. The spectral magnitude of these forces is studied with the help of a database that includes most of the available experimental data on direct or indirect force measurements. Different fluid mixtures, namely steam–water, air–water and various types of Freon, as well as different thermohydraulic or geometrical situations are included in the database. Using a formalism similar in principle to that used successfully in the modelling of buffeting in single-phase flows, the scaling of the data is undertaken. While dynamic pressure, viscosity or surface tension are found not to be relevant parameters, gravity forces allow us to define appropriate dimensionless spectra for all cases. The meaning of these parameters as well as the effects of flow regimes or fluid mixtures are discussed. Finally, an upper bound on the magnitude of these forces, which is of practical applicability, is proposed.     

ChemInform Abstract: Protonated Nitric Acid. Experimental Evidence for the Existence of Two Isomers.

The protonization of HNO₃ by H3⁺, CH₅⁺, and H₃O⁺ is studied by Fourier-transform (FT-ICR) and chemical ionization (CI) mass spectrometry.