全文获取类型
收费全文 | 1198篇 |
免费 | 34篇 |
国内免费 | 2篇 |
专业分类
化学 | 893篇 |
晶体学 | 13篇 |
力学 | 28篇 |
数学 | 127篇 |
物理学 | 173篇 |
出版年
2023年 | 8篇 |
2022年 | 16篇 |
2021年 | 48篇 |
2020年 | 37篇 |
2019年 | 26篇 |
2018年 | 20篇 |
2017年 | 16篇 |
2016年 | 30篇 |
2015年 | 49篇 |
2014年 | 39篇 |
2013年 | 89篇 |
2012年 | 96篇 |
2011年 | 90篇 |
2010年 | 48篇 |
2009年 | 64篇 |
2008年 | 98篇 |
2007年 | 71篇 |
2006年 | 56篇 |
2005年 | 38篇 |
2004年 | 57篇 |
2003年 | 41篇 |
2002年 | 29篇 |
2001年 | 21篇 |
2000年 | 22篇 |
1999年 | 16篇 |
1998年 | 8篇 |
1997年 | 15篇 |
1996年 | 8篇 |
1995年 | 5篇 |
1994年 | 9篇 |
1993年 | 6篇 |
1991年 | 2篇 |
1990年 | 10篇 |
1989年 | 6篇 |
1988年 | 4篇 |
1987年 | 2篇 |
1985年 | 8篇 |
1984年 | 1篇 |
1983年 | 5篇 |
1981年 | 2篇 |
1980年 | 3篇 |
1978年 | 2篇 |
1977年 | 2篇 |
1975年 | 1篇 |
1957年 | 1篇 |
1948年 | 1篇 |
1938年 | 1篇 |
1934年 | 2篇 |
1925年 | 2篇 |
1924年 | 2篇 |
排序方式: 共有1234条查询结果,搜索用时 390 毫秒
991.
Sushama Patil Julio Fernandes Rudreshwar Tangasali Irene Furtado 《Journal of Cluster Science》2014,25(2):423-433
In the present study we report the use of cells of a Haloarchaeon for the green synthesis of silver nanoparticles. Biosynthesis of AgNPs occurred within 30 s on exposure of cells of Haloferax alexandrinus to silver nitrate in direct sunlight. Maximum AgNPs production was achieved within 4 min of exposure of silver nitrate (0.05 %) to cells (5 mg/ml), at pH 7, at ambient day temperature (26–34 °C). The AgNPs had characteristic surface plasmon resonance at 420 nm in UV–Vis spectra. Spherical and irregular crystals ranging from 2 to 60 nm in size with an average size of 18 nm were observed in TEM analysis. The FTIR spectral analysis indicated involvement of N–H, –OH, C=O, C–O functional groups present in cells of Haloferax alexandrinus MTCC 3265. The biogenic AgNPs exhibited broad spectrum antimicrobial activity against human and mammalian pathogens, in the order of Pseudomonas aeruginosa ATCC 9027 > Bordetella bronchiseptica ATCC 4617 > Bacillus subtilis ATCC 6633 > Staphylococcus aureus ATCC 6538P > Staphylococcus epidermidis ATCC 12228 > Escherichia coli ATCC 8739 > Salmonella typhimurium ATCC 14028. 相似文献
992.
Luciana Carvalheira Paulo Bechara Dutra Paula Fernandes de Aguiar 《Journal of Radioanalytical and Nuclear Chemistry》2014,299(3):1805-1811
The HPLC method originally applied at the Nuclear Engineering Institute (IEN) for the radiochemical purity determination of 123iodine labeled m-iodobenzylguanidine (123I-mIBG) takes 18.5 min. The final product release also depends on this result, and to facilitate this stage, we aimed to decrease this analysis time. We also intended to use fewer toxic compounds, if feasible. The optimization approach used herein was a combination of factorial and mixture designs to study simultaneously the selected variables. Analysis time, resolution and chromatograms aspect were the measured responses. The qualitative analysis of these responses provided the best chromatographic separation conditions that were 52 mM KH2PO4 in a solution of ethanol and water (1:1), applying a flow rate of 0.50 mL min?1 and C18 column (4.6 × 250 mm, 5 μm). These optimum conditions not only decreased the analysis time in 61 %, but also allowed the reduction of mobile phase toxicity. To assure reliable data, method validation was performed for these conditions. The method has proved its specificity, the detection limit found was 3.70 × 10?4 MBq mL?1 and the quantification limit has corresponded to 1.11 × 10?3 MBq mL?1. Repeatability and intermediate precision has not exceeded 3 and 5 %, respectively, and the accuracy has matched the interval of 95–105 %. This new method has been routinely applied in the radiochemical purity determination of 123I-mIBG at IEN. 相似文献
993.
Alexandre Fernandes 《Results in Mathematics》2011,60(1-4):361-367
We show the existence of separating sets on a class of semi-weighted homogeneous hypersurface singularities with weights w 1 ≥ w 2 > w 3. In particular we show that these hypersurfaces are not metrically conical. 相似文献
994.
Pyruvate cellular uptake and enzymatic conversion probed by dissolution DNP‐NMR: the impact of overexpressed membrane transporters 下载免费PDF全文
Riccardo Balzan Laetitia Fernandes Laetitia Pidial Arnaud Comment Bertrand Tavitian Paul R. Vasos 《Magnetic resonance in chemistry : MRC》2017,55(6):579-583
Pyruvate membrane crossing and its lactate dehydrogenase‐mediated conversion to lactate in cells featuring different levels of expression of membrane monocarboxylate transporters (MCT4) were probed by dissolution dynamic nuclear polarization‐enhanced NMR. Hyperpolarized 13C‐1‐labeled pyruvate was transferred to suspensions of rodent tumor cell carcinoma, cell line 39. The pyruvate‐to‐lactate conversion rate monitored by dissolution dynamic nuclear polarization‐NMR in carcinoma cells featuring native MCT4 expression level was lower than the rate observed for cells in which the human MCT4 gene was overexpressed. The enzymatic activity of lactate dehydrogenase was also assessed in buffer solutions, following the real‐time pyruvate‐to‐lactate conversion speeds at different enzyme concentrations. Copyright © 2016 John Wiley & Sons, Ltd. 相似文献
995.
Kesia K. V. Castro Aneliese L. Figueiredo Amanda D. Gondim Ana C. F. Coriolano Ana P. M. Alves Valter J. Fernandes Jr. Antonio S. Araujo 《Journal of Thermal Analysis and Calorimetry》2014,117(2):953-959
The mesoporous material AlSBA-15 with Si/Al molar ratio of 25 was synthesized by the hydrothermal method and characterized by X-ray diffraction and nitrogen adsorption. This is a promising material in the field of catalysis, particularly for petroleum refining, since their mesopores facilitate the accessibility of large hydrocarbon molecules to the active sites of AlSBA-15, producing light hydrocarbons. In order to evaluating the catalytic activity of AlSBA-15, a sample of ca. 10 % in mass of this solid was physically mixed with atmospheric residue of petroleum (ATR) and heated in a thermobalance from room temperature up to 900 °C, under nitrogen atmosphere. From TG curves, it was observed a reduction in the onset temperature, when AlSBA-15 was used as catalyst for the degradation process of ATR. The kinetic model proposed by Ozawa–Flynn–Wall yielded some kinetic parameters to determine the apparent activation energy of the degradation, evidencing the efficiency of the mesoporous material, since there was a decrease in the activation energy for the catalytic degradation. The ATR alone and mixed with AlSBA-15 was also heated in a pyrolysis reactor coupled to a gas chromatograph and mass spectrometer (Py-GC/MS). From the chromatograms, it was observed an increase in the yield of hydrocarbons in the range of gasoline and diesel derived from the catalytic process. These results are due to the combination of the mesoporosity and acidity of the AlSBA-15 for application in the process of pyrolysis of hydrocarbons molecules constituents of the ATR. 相似文献
996.
A. J. D. Fernandes J. H. P. Barbosa O. G. Silva M. G. Fonseca L. H. N. Arakaki J. G. P. Espínola 《Journal of Thermal Analysis and Calorimetry》2011,106(2):463-467
Silica/monetite nanocomposites were synthesized through controlled hydrolysis of tetraethoxysilane at concentrations of 5,
10, 15, and 20% mol/mol of calcium phosphate forming the solids named CaPSil1, CaPSil2, CaPSil3, and CaPSil4, respectively.
XRD patterns showed formation of nanocomposites with a decrease in crystallinity. The NMR 29Si spectra suggested an increase in the content of incorporated silica with reduction of Q3 (–SiOH) signal, which contributes for mass loss, in agreement with thermogravimetry. The incorporation of silica increased
the chemical stability of the precursor phosphate in an acidic medium. 相似文献
997.
Rodney A. Fernandes Mahesh B. Halle Asim K. Chowdhury Arun B. Ingle 《Tetrahedron: Asymmetry》2012,23(1):60-66
A diastereoselective synthesis of (+)-nephrosterinic acid and (+)-protolichesterinic acid, common members of the paraconic acids is described. The synthesis is based on a diastereoselective orthoester Johnson–Claisen rearrangement of a (Z)-allyl alcohol with a vicinal dioxolane moiety as key steps. The synthesis is completed in 10 steps and with overall yields of 15.9% for (+)-nephrosterinic acid and 16.4% for (+)-protolichesterinic acid. 相似文献
998.
Joana P. B. Rodrigues ngela Fernandes Maria Inês Dias Carla Pereira Tnia C. S. P. Pires Ricardo C. Calhelha Ana Maria Carvalho Isabel C. F. R. Ferreira Lillian Barros 《Molecules (Basel, Switzerland)》2021,26(7)
Ruscus aculeatus L. is a subshrub used in traditional medicine in different parts of the world, namely in Europe and the Iberian Peninsula. According to reported folk knowledge, the aerial parts are mainly used as diuretics and the underground organs are used for the treatment of disorders of the urinary system and as a laxative. In this work, the aerial part and the roots and rhizomes of R. aculeatus were chemically characterized with regard to the content of phenolic compounds and bioactive properties. Aqueous (infusions and decoctions) preparations and hydroethanolic extracts from the two mentioned parts of the plant were prepared. Nine phenolic compounds were detected in all the extracts. Apigenin-C-hexoside-C-pentoside isomer II was the major compound in aqueous extracts and, in the hydroethanolic extract was quercetin-O-deoxyhexoside-hexoside followed by apigenin-C-hexoside-C-pentoside isomer II. All extracts revealed antioxidant activity and potential to inhibit some of the assayed bacteria; aqueous extracts of the aerial part and infusions of roots and rhizomes did not show cytotoxic effects on a non-tumor primary cell culture. This preliminary study provides suggestions of the biological potential associated with the empirical uses and knowledge of this species, in particular its bioactivities. 相似文献
999.
Rut Fernndez-Marín Susana C. M. Fernandes María A. Andrs Jalel Labidi 《Molecules (Basel, Switzerland)》2021,26(6)
Curcuma root (Curcuma longa L.) is a very important plant in gastronomy and medicine for its unique antiseptic, anti-inflammatory, antimicrobial and antioxidant properties. Conventional methods for the extraction of curcuma oil require long extraction times and high temperatures that can degrade the active substances. Therefore, the objectives of the present study were: (i) first, to optimize the extraction yield of curcuma oil by applying a Box-Behnken experimental design using surface response methodology to the microwave-assisted extraction (MAE) technique (the independent variables studied were reaction time (10–30 min), microwave power (150–200 W) and curcuma powder/ethanol ratio (1:5–1:20; w/v); and, (ii) second, to assess the total phenolic content (TPC) and their antioxidant activity of the oil (at the optimum conditions point) and compare with the conventional Soxhlet technique. The optimum conditions for the MAE were found to be 29.99 min, 160 W and 1:20 w/v to obtain an optimum yield of 10.32%. Interestingly, the oil extracted by microwave-assisted extraction showed higher TPC and better antioxidant properties than the oil extracted with conventional Soxhlet technique. Thus, it was demonstrated that the method applied for extraction influences the final properties of the extracted Curcuma longa L. oil. 相似文献
1000.
Danielle van der Westhuizen Dr. Cathryn A. Slabber Dr. Manuel A. Fernandes Daniël F. Joubert Dr. George Kleinhans Dr. C. Johan van der Westhuizen Dr. André Stander Prof. Orde Q. Munro Dr. Daniela I. Bezuidenhout 《Chemistry (Weinheim an der Bergstrasse, Germany)》2021,27(32):8295-8307
The syntheses of bis(triazolium)carbazole precursors and their corresponding coinage metal (Au, Ag) complexes are reported. For alkylated triazolium salts, di- or tetranuclear complexes with bridging ligands were isolated, while the bis(aryl) analogue afforded a bis(carbene) AuI-CNC pincer complex suitable for oxidation to the redox-stable [AuIII(CNC)Cl]+ cation. Although the ligand salt and the [AuIII(CNC)Cl]+ complex were both notably cytotoxic toward the breast cancer cell line MDA-MB-231, the AuIII complex was somewhat more selective. Electrophoresis, viscometry, UV-vis, CD and LD spectroscopy suggest the cytotoxic [AuIII(CNC)Cl]+ complex behaves as a partial DNA intercalator. In silico screening indicated that the [AuIII(CNC)Cl]+ complex can target DNA three-way junctions with good specificity, several other regular B-DNA forms, and Z-DNA. Multiple hydrophobic π-type interactions involving T and A bases appear to be important for B-form DNA binding, while phosphate O⋅⋅⋅Au interactions evidently underpin Z-DNA binding. The CNC ligand effectively stabilizes the AuIII ion, preventing reduction in the presence of glutathione. Both the redox stability and DNA affinity of the hit compound might be key factors underpinning its cytotoxicity in vitro. 相似文献