Enumeration of lactic acid bacteria: lacuna and improvement areas highlighted by proficiency testing

Accreditation and Quality Assurance - During the 2011 to 2018 food microbiology proficiency testing (PT) schemes organized by REQUASUD, a systematic analytical issue was detected. For the...     

Synthesis of the Zn1.9Cu0.1SiO4 pigment via the sol–gel and coprecipitation methods

Journal of Sol-Gel Science and Technology - The Zn1.9Cu0.1SiO4 pigment was obtained by two variants of “soft” chemistry methods: using sol–gel synthesis in ethanol from TEOS and...     

Purification of human recombinant anti-mullerian hormone and its derivatives

Anti-mullerian hormone (AMH) is a cytokine of transforming growth factor β (TGF-β) superfamily able to induce apoptosis in cells bearing specific AMH type II receptors (AMHRII). AMHRII is overexpressed in some malignant cells, so at present recombinant AMH (rAMH) is considered as a new candidate antineoplastic drug. The use of rAMH may be especially effective in case of such severe diseases as ovarian, prostate and breast cancer. However, the development of a new drug is hampered by the laboriousness of obtaining highly purified rAMH and by the lack of data about the pharmacological characteristics of rAMH derivatives. In this work, we obtained preparations of prohormone, half-cleaved rAMH and a C-terminal fragment of rAMH, which was confirmed by qualitative and quantitative analyses. To obtain rAMH and its derivatives we used a previously developed highly effective producer strain containing the optimized human AMH gene. The production process has been divided into several stages: (a) rAMH biosynthesis in the bioreactor; (b) culture media preparation; (c) purification of rAMH and its derivatives using immunoaffinity chromatography and reversed-phase HPLC; (d) identification of the purified proteins by immunoblotting and analytical reversed-phase HPLC; and (e) evaluation of the hormone forms activity. The obtained proteins may be used in preclinical trials and in vitro study of rAMH derivatives properties.     

Energy-Preserving Integrators Applied to Nonholonomic Systems

We introduce energy-preserving integrators for nonholonomic mechanical systems. We will see that the nonholonomic dynamics is completely determined by a triple \(({{\mathcal {D}}}^*, \varPi , \mathcal {H})\), where \({{\mathcal {D}}}^*\) is the dual of the vector bundle determined by the nonholonomic constraints, \(\varPi \) is an almost-Poisson bracket (the nonholonomic bracket) and \( \mathcal {H}: {{\mathcal {D}}}^*\rightarrow \mathbb {R}\) is a Hamiltonian function. For this triple, we can apply energy-preserving integrators, in particular, we show that discrete gradients can be used in the numerical integration of nonholonomic dynamics. By construction, we achieve preservation of the constraints and of the energy of the nonholonomic system. Moreover, to facilitate their applicability to complex systems which cannot be easily transformed into the aforementioned almost-Poisson form, we rewrite our integrators using just the initial information of the nonholonomic system. The derived procedures are tested on several examples: a chaotic quartic nonholonomic mechanical system, the Chaplygin sleigh system, the Suslov problem and a continuous gearbox driven by an asymmetric pendulum. Their performance is compared with other standard methods in nonholonomic dynamics, and their merits verified in practice.

     

New mesomorphic organocyclosiloxanes II. Thermal behaviour and mesophase structure of organocyclohexasiloxanes

Investigations of thermotropic phase transitions performed on organocyclosiloxanes [PhSi(O)OSiR]₆, where R is Me₃, Me₂(CH₂Cl) or Me₂(CH≃CH₂), have revealed that all these hexamers are mesomorphic compounds. The hexamers exhibit uncommon polymesomorphic behaviour forming two quite different mesomorphic structures. The molecular arrangement in the low temperature (LT) modification is characterized by two-dimensional (2D) long-range order with hexagonal packing. The X-ray diffraction pattern and peculiarities of molecular packing in the crystal lead us to suggest that the LT-mesophase is columnar, presumably of the Col_hd type. The LT-mesophase is formed by dimeric moieties, which associate with each other in column-like substructures, the ring planes not orthogonal to the stack axis. The high temperature (HT) mesophase is a plastic crystal (3D-order), where molecules take up positions in a face-centred cubic lattice. This is a very uncommon example of thermal behaviour for plastic crystals that provides a unique opportunity to bridge the gap between plastic crystalline and liquid crystalline mesomorphic behaviour. The thermal and structural properties of the mesophases depend upon the type of side groups of the hexamers. The size of the ring also affects the phase behaviour and the mesomorphic structure. This conclusion is consistent with data obtained by us earlier for cyclotetrasiloxanes.     

Equation of state and thermal stability of Al3BC

The lattice parameters of Al₃BC have been measured up to 5 GPa at ambient temperature using energy-dispersive X-ray powder diffraction with synchrotron radiation. A fit to the experimental p-V data using Birch-Murnaghan equation of state gives values of the Al₃BC bulk modulus 116(4) GPa and its first pressure derivative 9(2). In the 1.6-4.8 GPa range at temperatures above 1700 K Al₃BC undergoes incongruent melting that results in the formation of Al₃BC₃, AlB₂ and liquid aluminum.     

Studying Baskakov–Durrmeyer operators and quasi-interpolants via special functions

We prove that the kernels of the Baskakov–Durrmeyer and the Szász–Mirakjan–Durrmeyer operators are completely monotonic functions. We establish a Bernstein type inequality for these operators and apply the results to the quasi-interpolants recently introduced by Abel. For the Baskakov–Durrmeyer quasi-interpolants, we give a representation as linear combinations of the original Baskakov–Durrmeyer operators and prove an estimate of Jackson–Favard type and a direct theorem in terms of an appropriate K-functional.