Determination of free carrier protein in protein-polysaccharide conjugate vaccines by micellar electrokinetic chromatography

Protein-polysaccharide conjugate vaccines offer the prospect of reducing morbidity and mortality due to bacterial pneumonia and meningitis but, because of their size and heterogeneity, are often a challenge to characterize by traditional analytical methods. Vaccines consisting of Streptococcus pneumoniae, or Neisseria meningiditis polysaccharide covalently linked to formaldehyde-inactivated diphtheria toxoid carrier protein were resolved from non-conjugated toxoid by micellar electrokinetic chromatography. Separation was achieved using alkaline sodium borate solutions containing sodium dodecyl sulfate in excess of the critical micellar concentration. No sample pretreatment was required prior to analysis. Diphtheria toxoid peak migration times were highly reproducible. Measurement of absolute toxoid peak area showed poor precision, but good precision was observed when peak area was normalized against an internal standard (myoglobin). Good linearity was observed over useful ranges of both protein content and injection time.     

Detection of stable isotopes with A (n, α) nuclear reaction: Application to the measurement of unidirectional fluxes of borate in a plant

The unidirectional influx of borate has been studied inLemna minor plants with the aid of purified boron isotopes,¹⁰B and¹¹B. Isotope¹⁰B was detected specifically by nuclear reaction¹⁰B(n, α)⁷Li in the presence of “homogeneous” detectors. Despite technical difficulties in performing the¹⁰B-estimations in the plant samples themselves, the results obtained here were consistent with those of efflux experiments, published previously, where the¹⁰B-measurements were performed in the external solution. Kinetic parameters of borate transports inLemna minor have been calculated.     

Etude des acides aminés sur une goutte de solution aqueuse par spectrographie infrarouge

Résumé L'auteur donne les spectres infrarouges des solutions neutres, acides et basiques d'un certain nombre d'amino-acides; il fournit les bandesclés utiles pour un futur dosage et examine l'influence de l'alanine et de l'acide glutamique quand on les mélange au glycocolle.

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Summary The infra red spectra of neutral, acid, and basic solutions of a certain number of amino, acids are given. The author also provides the key bands to be used for a future determination. He examines the influence of alanine and glutamic acid when they are mixed with glycine.

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Zusammenfassung Die Infrarotspektren neutraler, saurer und basischer Lösungen einer Reihe von Aminosäuren werden angegeben. Die für die künftige Bestimmung geeigneten Schlüsselbanden wurden festgestellt und der Einfluß von Alanin und Glutaminsäure in Mischungen mit Glykokoll untersucht.

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A M. le Professeur de l'Université Dr.Hans Lieb à l'occasion de son 70ème anniversaire.     

Synthesis and structural characterization of a uranyl(VI) complex possessing unsupported unidentate thiolate ligands

The synthesis and structural characterization of the first example of a uranyl(VI) complex possessing unsupported unidentate thiolate ligands, UO2(S-2,6-Cl2C6H3)2L2 (2b, L=N,N-diisobutylisopropylamide), are reported. Isolation of 2b as a stable mononuclear complex is provided by the alkyl substituents of the organic amide ligands, which offer enhanced solubility, electron-releasing properties, and steric protection to help saturate the uranyl(VI) coordination sphere.     

Analogues of cytotoxic squamocin using reliable reactions: new insights into the reactivity and role of the α,β-unsaturated lactone of the annonaceous acetogenins

A small library of squamocin analogues has been prepared and screened biologically (cytotoxicity, inhibition of mitochondrial complex I and complex III). To centre diversity on a crucial part of the molecule (i.e., the α,β-unsaturated lactone), an original and reliable lactone opening reaction has been discovered and exploited among other efficient reactions.     

Sur la thermogravimétrie des précipités analytiques : Dosage de L'ammonium

The temperature of 110° C is quite suitable for drying ammonium chloride, prior to weighing. If the residual platinum from the pyrolysis of ammonium chloroplatinate is weighed it. is necessary to ignite, either between 407° and 538° C, or above 811° C.