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11.
Stevens SM Chung AY Chow MC McClung SH Strachan CN Harmon AC Denslow ND Prokai L 《Rapid communications in mass spectrometry : RCM》2005,19(15):2157-2162
A fluorescent affinity tag (FAT) was synthesized and was utilized to selectively modify phosphorylated serine and threonine residues via beta-elimination and Michael addition chemistries in a 'one-step' reaction. This labeling technique was used for covalent modification of both phosphoproteins and phosphopeptides, allowing identification of these molecular species by fluorescence imaging after solution- or gel-based separation methods. In addition to the strong fluorescence of the rhodamine tag, a commercially available antibody can be used to enrich low-abundance post-labeled phosphopeptides present in complex mixtures. Application of this methodology to phosphorylation-site mapping has been evaluated for a phosphoprotein standard, bovine beta-casein. Initial results demonstrated low femtomole detection limits after fluorescence image analysis of FAT-labeled proteins or peptides. 相似文献
12.
(Z, E)-9, 11-Tetradecadienyl-l-acetate (1), a major component of the sex pheromone of Spodoptera litura (F.), and (Z, E}-9, 11-pentadecadienyl-1-acetate (2) were synthesized by the Wittig reaction between (E)-2-alkenal (3) and the ylid derived from 9-hydroxynonyltriphenylphosphonium bromide (4). 相似文献
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14.
Rate constants for quenching of 1O2 by a number of strained molecules have been determined by the competitive rubrene photooxidation method; the rate constant for quenching by Q may be evaluated by adaption of a kinetic analysis already in the literature for the rubrene photooxidation method. 相似文献
15.
Chow CK 《Physical review D: Particles and fields》1995,51(7):3541-3543
16.
R. F. Kiefl J. Sonier D. A. Bonn J. H. Brewer J. F. Carolan K. H. Chow P. Dosanjh W. N. Hardy Ruixing Liang W. A. MacFarlane P. Mendels G. D. Morris T. M. Riseman J. W. Schneider 《Hyperfine Interactions》1994,86(1):537-542
We report precision transverse fieldSR measurements of the internal field distribution in the vortex state of crystalline YBa2Cu3O6.95. A novel low background apparatus was used to study a mosaic sample of three high quality single crystals (Tc=93.3K). The observed frequency spectra in magnetic fields of 5kG and 15 kG applied along the c-axis have the characteristic features expected for a regular vortex lattice with some additional broadening. From a preliminary analysis we find that [(0)/(T)]2 has a linear temperature coefficient forT<30 K. Such a term is inconsistent with simple s-wave pairing in the superconducting state. These results support recent microwave measurements of(T) on similar crystals in zero applied field but differ significantly from previousSR reports on sintered powders and crystals with lower Tc.This research was supported by the Natural Sciences and Engineering Research Council of Canada. 相似文献
17.
R. L. Lichti K. H. Chow D. W. Cooke S. F. J. Cox E. A. Davis R. C. DuVarney T. L. Estle B. Hitti S. R. Kreitzman R. Macrae C. Schwab A. Singh 《Hyperfine Interactions》1994,86(1):711-716
Longitudinal-field muon-spin depolarization rates in high-purityGe and semiinsulatingGaAs are reported and compared to similar data for intrinsicSi. Depolarization onset temperatures provide a comparison of charge carrier concentrations leading to rapid charge exchange and a shift in the onset, for n-typeGaAs verifies anelectron process. The temperature dependence of the low-field rate constants imply more complicated dynamics inGe than observed earlier inSi. Features near 750K inGaAsTe appear consistent with dissociation of aMu-Te pair.This work was supported by the US National Science Foundation (DMR-8917639 [TLE, BH]), the Science and Engineering Research Council of the UK (EAD, AS, SFJC), the Robert A. Welch Foundation (D-1053 [RLL], and C-1048 [TLE]), and a NATO Collaborative Research Grant (RLL, SFJC, RCD, CS). We wish to thank D.A. Vanderwater of Hewlett Packard for providing theGaAsTe sample. 相似文献
18.
B. Hitti S. R. Kreitzman T. L. Estle R. L. Lichti K. H. Chow J. W. Schneider C. D. Lamp P. Mendels 《Hyperfine Interactions》1994,86(1):673-679
The radio frequencySR technique developed at TRIUMF was used to measure the temperature dependence of the diamagnetic muon, Mu, and Mu* amplitudes in silicon between 10 K and 500 K. Six samples doped with phosphorus (n-type) and boron (p-type) in the concentration range 1011 to 1015 cm–3 were studied. In pure Si a very good fit over the whole temperature range is obtained from a model that includes the ionization of Mu* and Mu to a bond centered
+ followed at high temperature by charge exchange involving Mu. 相似文献
19.
R. Kadono J. H. Brewer K. Chow S. R. Kreitzman Ch. Niedermayer T. M. Riseman J. W. Schneider T. Yamazaki 《Hyperfine Interactions》1994,85(1):259-264
The electric quadrupole interaction of55Mn nuclei was studied in the weakly ferromagnetic system MnSi using muon level-crossing resonance (LCR) technique. The temperature dependence of the electric field gradient (EFG) shows a critical behavior near the ferromagnetic transition temperature, indicating that the EFG due to the conduction electron is strongly correlated with the magnetic susceptibility in the itinerant electron magnetism. The temperature dependence of EFG is in reasonable agreement with the self-consistent renormalization theory developed by Moriya and coworkers.We gratefully acknowledge helpful discussion with Dr. N. Nishida. We also wish to thank Keith Hoyle and Curtis Ballard for technical support. 相似文献
20.