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61.
The peptide mass fingerprinting technique is commonly used for identifying proteins analyzed by mass spectrometry (MS) after enzymatic digestion. Our goal is to build a theoretical model that predicts the mass spectra of such digestion products in order to improve the identification and characterization of proteins using this technique. We present here the first step towards a full MS model. We have modeled MS spectra using the atomic composition of peptides and evaluated the influence that this composition may have on the MS signals. Peptides deduced from the SWISS-PROT protein sequence database were used for the calculation. To validate the model, the variability of the peptide mass distribution in SWISS-PROT was compared to two theoretical, randomly generated databases. Functions have been built that describe the behavior of the isotopic distribution according to the mass of peptides. The variability of these functions was analyzed. In particular, the influence of sulfur was studied. This work, while representing only a first step in the construction of an MS model, yields immediate practical results, as the new isotopic distribution model significantly improves peak detection in MS spectra used by protein identification algorithms. 相似文献
62.
Prof. Dr. E. Binz 《Monatshefte für Mathematik》1980,89(4):275-288
OnE(M,
n
), the Fréchet-manifold of all smooth embeddings of a smooth, compact, closed, orientable manifoldM (of dimensionn-1) into
n
two natural metricsG and
are considered. The metric
plays a central rôle in elasticity theory. Using a generalised notion of the Fréchet derivative their respective spraysS and
and the correspoonding Levi-Civita connections are computed. BothS and
are smooth in a well defined sense. In contrast toS the spray
turns out to be trivial. 相似文献
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Ohne Zusammenfassung 相似文献
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