Simultaneous quantitation of the highly lipophilic atovaquone and hydrophilic strong basic proguanil and its metabolites using a new mixed-mode SPE approach and steep-gradient LC

A bioanalytical method is described for the simultaneous quantitative analysis of the highly lipophilic atovaquone and the strong basic proguanil with metabolites in plasma. The drugs are extracted from protein precipitated plasma samples on a novel mixed-mode solid-phase extraction (SPE) column containing carboxypropyl and octyl silica as functional groups. The analytes are further separated and quantitated using a steep-gradient liquid chromatographic method on a Zorbax SB-CN column with UV detection at 245 nm. Two different internal standards (IS) are used in the method to compensate for both types of analytes. A structurally similar IS to atovaquone is added with acetonitrile to precipitate proteins from plasma. A structurally similar IS to proguanil and its metabolites is added with phosphate buffer before samples are loaded onto the SPE columns. A single elution step is sufficient to elute all analytes. The method is validated according to published guidelines and shows excellent performance. The within-day precisions, expressed as relative standard deviation, are lower than 5% for all analytes at three tested concentrations within the calibration range. The between-day precisions are lower than 13% for all analytes at the same tested concentrations. The limit of quantitation is 25 nM for the basic substances and 50 nM for atovaquone. Several considerations regarding development and optimization of a method for determination of analytes with such a difference in physiochemical properties are discussed.     

Reversed-phase liquid chromatographic method for the simultaneous determination of the antimalarial drugs sulfadoxine, pyrimethamine, mefloquine and its major carboxylic metabolite in plasma.

A high-performance liquid chromatographic (HPLC) method for the simultaneous determination of sulfadoxine, pyrimethamine, mefloquine and the carboxylic metabolite of mefloquine in plasma is described. After the proteins have been precipitated with a combination of zinc sulphate and acetonitrile containing two internal standards, pyrimethamine and mefloquine are extracted as bases and sulfadoxine and the carboxylic metabolite of mefloquine as ion-pairs with tetrabutylammonium. The drugs are separated by HPLC on a 3 micron octadecylsilica column with ultraviolet detection at 229 nm. The method is simple and reliable and enables the simultaneous determination of the drugs in 600-microliter plasma samples with a sensitivity suitable for standard drug monitoring purposes.     

非平衡态/不可逆过程的热力学理论(Ⅱ)

<正> 7 解的唯一性 如果不考虑非平衡态和不可逆性,则等能密度理论中的基本命题与经典弹性理论中的在形式上完全相同。无论整个系统的动能是大还是小,图6中(sumform)_o上的面力T_i和在(sumform)_c和在的位移u_i将始终在空间坐标系x_i和时刻i下给出。与一般力学中相同,必须知道有关位移u_i及其时间导数的初始条件。它们的影响则可通过(38)和(39)向等能面转化,从而得到     

Dependence of 14 MeV radiative neutron capture on mass number

The 14 MeV neutron radiative capture cross section has been measured for six nuclei in the mass range from A = 155 to A = 238. The integrated radiative cross sections are found to have a value near 1 mb. These results are at variance with the results obtained by the activation technique. Detailed captude γ-ray spectra have been measured for one closed-shell and two deformed nuclei to illustrate their similarity in shape and magnitude.     

Radiative capture of fast neutrons by 89Y and 140Ce

The γ-ray spectra from the reactions ⁸⁹Y(n, γ)⁹⁰Y and ¹⁴⁰Ce(n, γ)¹⁴¹Ce have been measured in the neutron energy range of 6.2–15.6 MeV. The pulse-height spectra were recorded with NaI(Tl) spectrometers and time-of-flight techniques were used to improve signal-to-background ratio. Capture cross sections were determined for γ-ray transitions to the two $\text{2}\text{d}{}_{\mathrm{<mtext>5</mtext><mtext>2</mtext>}}$ levels at 0 and 203 keV of ⁹⁰Y and to the $\text{2}\text{f}{}_{\mathrm{<mtext>7</mtext><mtext>2</mtext>}}$ ground state of ¹⁴¹Ce as well as integrated cross sections to bound states in these nuclei. The observed γ-ray spectra and partial radiative capture cross sections were compared with predictions of the direct-semidirect capture theory. The resonance behaviour with neutron energy of both the ground-state and integrated partial capture cross sections shows the validity of the semidirect model for ⁸⁹Y and ¹⁴⁰Ce in the region of neutron energy encompassing the giant-dipole resonance. The observed symmetry of the cross sections about the peak of the resonance argues strongly for the complex form of the particle-vibration coupling interaction. A detailed comparison of the predictions of the DSD model using the complex coupling interaction shows that the capture cross sections are relatively insensitive to the real part of the interaction.