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171.
Edward-Sam Edwin Prabhakaran Vasantha-Srinivasan Sengottayan Senthil-Nathan Muthiah Chellappandian Sengodan Karthi Radhakrishnan Narayanaswamy Vethamonickam Stanley-Raja Haridoss Sivanesh Ramakrishnan Ramasubramanian Asma A. Al-Huqail Faheema Khan Patcharin Krutmuang Ahmed Abdel-Megeed Aml Ghaith Chae-Hoon Paik 《Molecules (Basel, Switzerland)》2021,26(19)
Spodoptera litura Fab. is a polyphagous pest causing damage to many agriculture crops leading to yield loss. Recurrent usage of synthetic pesticides to control this pest has resulted in resistance development. Plant-derived diterpenoid compound andrographolide was isolated from the leaves of Andrographis paniculata. It was analysed by gas chromatography-mass spectroscopy and quantified by HPLC. Nutritional indices and digestive enzymatic profile were evaluated. Third, fourth and fifth instar larvae were treated with different concentrations of andrographolide. At 3, 6 and 9 ppm-treated concentrations the larvae showed decreased RGR, RCR, ECI, ECD values with adverse increase in AD. The digestive enzymes were significantly inhibited when compared with control. Conspicuously, andrographolide showed pronounced mortality of S. litura by inhibition of enzyme secretion and intake of food. The binding ability of andrographolide with CYTP450 showed high affinity with low binding energy. Andrographolide has the potential to be exploited as a biocontrol agent against S. litura as an eco-friendly pesticide. 相似文献