高效毛细管区带电泳分离Cu(Ⅱ),Zn(Ⅱ),Pb(Ⅱ)和Al(Ⅲ)-EDTA配合物

建立了用高效毛细管区带电泳（ＣＺＥ）分离Ｃｕ（Ⅱ），Ｚｎ（Ⅱ），Ｐｂ（Ⅱ）和Ａｌ（Ⅲ）－ＥＤＴＡ配合物的方法。对影响Ｍ－ＥＤＴＡ配合物分离的主要因素，如ｐＨ值、ＥＤＴＡ用量和阳离子表面活性剂用量等进行了讨论。采用控制加权可变步长单纯形算法动态寻优对影响［Ａｌ－ＥＤＴＡ］－形成的因素进行了优化。     

固相法氯化聚乙烯对PVC／LLDPE共混体系性能和形态的影响

采用固相法氯化聚乙烯（ＣＰＥ）对聚氯乙烯／线型低密度聚乙烯（ＰＶＣ／ＬＬＤＰＥ）共混体系进行增容改性。扫描电子显微镜、透射电子显微镜、动态力学分析和力学性能测试结果表明，ＣＰＥ对ＰＶＣ／ＬＬＤＰＥ共混体系具有很好的增容作用。     

高效毛细管区带电泳分离Cu(Ⅱ),Zn(Ⅱ),Pb(Ⅱ)和Al(Ⅲ)-EDTA配合物

 建立了用高效毛细管区带电泳（ＣＺＥ）分离Ｃｕ（Ⅱ），Ｚｎ（Ⅱ），Ｐｂ（Ⅱ）和Ａｌ（Ⅲ）－ＥＤＴＡ配合物的方法。对影响Ｍ－ＥＤＴＡ配合物分离的主要因素，如ｐＨ值、ＥＤＴＡ用量和阳离子表面活性剂用量等进行了讨论。采用控制加权可变步长单纯形算法动态寻优对影响［Ａｌ－ＥＤＴＡ］－形成的因素进行了优化。     

聚氯乙烯／线性低密度聚乙烯共混体系的相容性

用动态力学分析（ＤＭＡ）和傅利叶变换红外光谱（ＦＴＩＲ）研究了氢化聚丁二烯－ｂ－聚甲基丙烯酸甲酯（ＨＰＢＤ－ｂ－ＰＭＭＡ）共聚物增容剂对聚氯乙烯（ＰＶＣ）与线性低密度聚乙烯（ＬＬＤＰＥ）共混体系的增容作用．增容剂使共混物中两相的玻璃化温度发生变化，说明其相容性增加．ＦＴＩＲ的结果表明，增容剂中羰基与ＰＶＣ的α氢形成氢键，使ＣＯ，Ｈ─Ｃ及Ｃ─Ｃｌ的振动频率变化，峰形加宽．     

脑显像剂~（99m）Tc－ECD的制备研究

~（９９ｍ）Ｔｃ－ＥＣＤ是目前脑显像剂中一种较为理想的放射性诊断药物。本文介绍了从基本原料（Ｌ）半胱氨酸开始合成配体ＥＣＤ（Ｎ，Ｎ＇－乙撑－双－Ｌ半胱氨酸乙酯），采用锡作还原剂实现了一步法~（９９ｍ）Ｔｃ标记ＥＣＤ，并对制备的~（９９ｍ）Ｔｃ－ＥＣＤ进行了初步动物实验以评价其生物学性能。     

HPLC检测器的线性动态范围及其测试方法的研究

简单论述了ＨＰＬＣ检测器（ＨＰＬＣＤ）的线性动态范围（ＬＤＲ）的重要性，提出了对光学式ＨＰＬＣＤ的ＬＤ测试的一种新方法，同时对有关问题进行了讨论。     

脱氧核糖核酸变性和损伤的吸附伏安法研究

本文用汞电极（ＨＭＤＥ）二次导数阴极吸附伏安（ＳＤ－ＡｄＣＳＶ）和碳电极（ＧＣＥ、ＣＰＥ）导数循环伏安（ＦＤ－ＣＶ）法研究了核酸受热、紫外线、超声波和丝裂霉素Ｃ（ＭＭＣ）作用下的变性作用。在０．１ｍｏｌ／Ｌ（Ｋ２ＨＰＯ４＋ＫＨ２ＰＯ４）－０．１ｍｏｌ．Ｌ ＮａＣｌ（ｐＨ７．０）底液中，吸附的单股（ｓｓ－）和双螺旋（ｄｓ－）ＤＮＡ分别在ＨＭＤＥ上得到特征还原峰Ｐ３和Ｐ２，和在碳电极上得到氧化峰Ａ。物     

BOND LENGTHS OF BUCKY-BALLS C_(60), C_(240), C_(540), AND C_(960)

ＢＯＮＤＬＥＮＧＴＨＳＯＦＢＵＣＫＹ－ＢＡＬＬＳＣ_（６０），Ｃ_（２４０），Ｃ_（５４０），ＡＮＤＣ_（９６０）￥ＬｅｉＬＩＵ；ＫａｉＴａｉＣＨＥＮ；ａｎｄＹｕＦｅｎＬＩ（ＤｅｐａｒｔｍｅｎｔｏｆＰｈｙｓｉｃｓ，ＦｕｄａｎＵｎｉｖｅｒｓｉｔｙ，Ｓｈａｎ...     

刚性链嵌段共聚物的研究（Ⅳ）—聚醚醚酮／苯酞圈型聚芳醚砜…

以聚醚醚酮（ＰＥＥＫ）和苯酞圈型聚芳醚砜（ＰＤＣ）齐聚物为原料，通过溶液缩聚法制备了ＰＥＥＫ／ＰＤＣ有规嵌段共聚物系列样品。经ＤＳＣ、ＷＡＸＤ、ＴＧＡ、ＤＭＡ等方法研究表明：共聚物没有微相分离现象；其结晶属于简单正交晶系，ＰＤＣ含量对共聚物结晶行为产生很大影响；热稳定性随ＰＤＣ含量增加而明显降低；共聚物具有优异的力学性能，拉伸强度和储能模量高于ＰＥＥＫ。     

Author Index Vol. 7, 1996

ＡｕｔｈｏｒＩｎｄｅｘＶｏｌ．７，１９９６￥／／ＡｌＸ．Ｃ．（７１）ＣＨＥＮＮ．Ｙ．（７８１）ＤＥＮＧＣ．Ｈ．（５６９）ＦＵＸ．Ｙ．（６３１）ＡＮＬ．Ｄ．（７３７）ＣＨＥＮＰ．（３６１）ＤＥＮＧＤ．Ｌ．（８４５）ＦＵＹ．Ｈ．（８５３）ＢＡＩＤ．Ｌ．（...     

一种基于共振角度测量的小型SPR传感器

合成了一种光学有机玻璃并进行了性能表征，用其制作了一种不规则六面体光学棱镜，采用这种光学棱镜，以发光二极管为光源，线阵CCD为检测器，组装了一种共振角度同时测量的小型SPR传感装置，讨论了影响该传感器灵敏度，分辨率和线性范围的因素，将其用于乙醇和磷酸二氢钾的检测，获得了较好的结果。     

Determination of Rutin with UV-Vis Spectrophotometric and Laser-Induced Fluorimetric Detections Using a Non-Scanning Spectrometer

This study presents a linear charge-coupled device (CCD) spectrometer and its application to quantitative determination of rutin with UV-Vis spectrophotometry and fluorimetry detections. The spectrometer can obtain spectrum with the range of 271.44 nm from one exposure and the 1.7 nm resolution. With the same spectrometer, the determination of rutin in rutin tablet was successfully studied by measuring the absorption and laser-induced fluorescence emission. The data shows that there is no significant difference for determining rutin between the two methods. The result implies that the spectrometer, which realizes two different analytical methods, can be widely used in trace analysis.     

微型CCD光谱仪在光谱分析中的应用

现代分析仪器的一个重要发展趋势是仪器的小型化（miniaturization）、微型化（microminiaturiza．ion），甚至于芯片实验室（lab-on-a-chip；或称微全分析系统，micro total analysis system，μ-TAS）。因此，许多科学工作者在提高光谱分析仪器的基本部件——辐射源、样品室、分光元件和检测器的性能和减小其体积方面做了大量的研究工作。半导体检测器在光谱分析中的应用越来越广泛，在实验室和实际工作中常常会接触到摄谱仪、光电倍增管（photomultiplier tube，PMT）和电荷耦合器件（charge coupled device，CCD）等，本文介绍微型CCD光谱仪在本科教学中的一些应用。     

Single-step calibration, prediction and real samples data acquisition for artificial neural network using a CCD camera

An artificial neural network (ANN) model is developed for simultaneous determination of Al(III) and Fe(III) in alloys by using chrome azurol S (CAS) as the chromogenic reagent and CCD camera as the detection system. All calibration, prediction and real samples data were obtained by taking a single image. Experimental conditions were established to reduce interferences and increase sensitivity and selectivity in the analysis of Al(III) and Fe(III). In this way, an artificial neural network consisting of three layers of nodes was trained by applying a back-propagation learning rule. Sigmoid transfer functions were used in the hidden and output layers to facilitate nonlinear calibration. Both Al(III) and Fe(III) can be determined in the concentration range of 0.25-4 μg ml⁻¹ with satisfactory accuracy and precision. The proposed method was also applied satisfactorily to the determination of considered metal ions in two synthetic alloys.     

Integration of cell phone imaging with microchip ELISA to detect ovarian cancer HE4 biomarker in urine at the point-of-care

Ovarian cancer is asymptomatic in the early stages and most patients present with advanced levels of disease. The lack of cost-effective methods that can achieve frequent, simple and non-invasive testing hinders early detection and causes high mortality in ovarian cancer patients. Here, we report a simple and inexpensive microchip ELISA-based detection module that employs a portable detection system, i.e., a cell phone/charge-coupled device (CCD) to quantify an ovarian cancer biomarker, HE4, in urine. Integration of a mobile application with a cell phone enabled immediate processing of microchip ELISA results, which eliminated the need for a bulky, expensive spectrophotometer. The HE4 level detected by a cell phone or a lensless CCD system was significantly elevated in urine samples from cancer patients (n = 19) than healthy controls (n = 20) (p < 0.001). Receiver operating characteristic (ROC) analyses showed that the microchip ELISA coupled with a cell phone running an automated analysis mobile application had a sensitivity of 89.5% at a specificity of 90%. Under the same specificity, the microchip ELISA coupled with a CCD had a sensitivity of 84.2%. In conclusion, integration of microchip ELISA with cell phone/CCD-based colorimetric measurement technology can be used to detect HE4 biomarker at the point-of-care (POC), paving the way to create bedside technologies for diagnostics and treatment monitoring.     

CCD camera full range pH sensor array

Changes in colors of an array of optical sensors that responds in full pH range were recorded using a CCD camera. The data of the camera were transferred to the computer through a capture card. Simple software was written to read the specific color of each sensor. In order to associate sensor array responses with pH values, a number of different mathematics and chemometrics methods were investigated and compared. The results show that the use of “Microsoft Excel's Solver” provides results which are in very good agreement with those obtained with chemometric methods such as artificial neural network (ANN) and partial least square (PLS) methods.     

CCD近红外光谱分析技术在测定红烟硝酸中的应用

从化学计量学角度研究了CCD近红外光谱技术在无机物领域分析中的应用。使用偏最小二乘法(PLS)建立了红烟硝酸的密度、水分含量等五项指标的快速测定模型,验证了近红外光谱测定红烟硝酸各项指标的可靠性。试验证明,CCD近红外光谱方法具有重现性好、分析速度快、使用样品量少、可以在线分析等优点,可以用于无机物的常规分析。     

Detection of Multiple Herbicide Residues Using A Planar Array Evanescent Field Immunosensor

ABSTRACT

A planar array evanescent immunosensor, equipped with a charge-coupled device (CCD) as a detector was used to simultaneously detect pesticides, paraoxon, carbofuran and tetrachloroethylene. Wells approximately 2.5 mm in diameter were formed on glass slides using a photoactivated optical adhesive. Coating haptens were covalently attached to the bottoms of the circular wells to form a sensing surface. The detection of the hapten was completed through competitive immuno-reaction between the coasting haptens, and haptens in solution, with the corresponding antibody in solution. The identification and amount of antibody bound at each location on the slide were determined by quantitative image analysis. Concentrations as low as 0.5ng/mL of paraoxon, 0.Ing/mL of carbofuran, and 0.2ng/mL of tetrachloroethlene could be measured.     

烟用香精的气相色谱法质量控制研究

 采用配有氢焰检测器或催燃检测器的气相色谱仪，对烟用香精进行了快速分析。方法操作简便，结果可靠，可作为烟用香精原料、产品质量监控的有效手段。     

The use of experimental design in the development of an HPLC-ECD method for the analysis of captopril

An accurate, sensitive and specific high performance liquid chromatography-electrochemical detection (HPLC-ECD) method that was developed and validated for captopril (CPT) is presented. Separation was achieved using a Phenomenex^® Luna 5 μm (C₁₈) column and a mobile phase comprised of phosphate buffer (adjusted to pH 3.0): acetonitrile in a ratio of 70:30 (v/v). Detection was accomplished using a full scan multi channel ESA Coulometric detector in the “oxidative-screen” mode with the upstream electrode (E₁) set at +600 mV and the downstream (analytical) electrode (E₂) set at +950 mV, while the potential of the guard cell was maintained at +1050 mV. The detector gain was set at 300. Experimental design using central composite design (CCD) was used to facilitate method development. Mobile phase pH, molarity and concentration of acetonitrile (ACN) were considered the critical factors to be studied to establish the retention time of CPT and cyclizine (CYC) that was used as the internal standard. Twenty experiments including centre points were undertaken and a quadratic model was derived for the retention time for CPT using the experimental data. The method was validated for linearity, accuracy, precision, limits of quantitation and detection, as per the ICH guidelines. The system was found to produce sharp and well-resolved peaks for CPT and CYC with retention times of 3.08 and 7.56 min, respectively. Linear regression analysis for the calibration curve showed a good linear relationship with a regression coefficient of 0.978 in the concentration range of 2-70 μg/mL. The linear regression equation was y = 0.0131x + 0.0275. The limits of detection (LOQ) and quantitation (LOD) were found to be 2.27 and 0.6 μg/mL, respectively. The method was used to analyze CPT in tablets. The wide range for linearity, accuracy, sensitivity, short retention time and composition of the mobile phase indicated that this method is better for the quantification of CPT than the pharmacopoeial methods.