Variety characteristics of soybean seeds in relation to protein and oil contents and activities of proteinase inhibitors

The protein and oil contents and the activity of proteinase inhibitors in six varieties of soybean have been studied. It has been found that the specific amidase activity of trypsin inhibitors ranges from 170 to 320 nominal units. Electrophoretic results indicates the presence in the water-soluble fraction of seven or eight components possessing inhibitor activity in relation to trypsin and chymotrypsin.Institute of Plant Physiology and Biophysics, Academy of Sciences of the Tadzhik SSR, Dushanbe. Institute of Molecular Biology, Academy of Sciences of the USSR, Moscow. Translated from Khimiya Prirodnykh Soedinenii, No. 6, pp. 761–765, November–December, 1984.     

Amounts of protein and oil and activity of the trypsin inhibitor in different varieties of soybean

Fifteen varieties of soybean have been investigated for the amounts of protein, oil, and trypsin inhibitor that they contain. A high thermal stability of the latter has been detected. Information is given on the kinetics of the inhibition of the amidase and proteinase activity of trypsin. It has been shown that the trypsin inhibitor consists of a mixture of six proteins.Institute of Plant Biophysics and Physiology, Academy of Sciences of the Tadzhik SSR, Dushanbe. Institute of Molecular Biology, Academy of Sciences of the USSR, Moscow. Translated from Khimiya Prirodnykh Soedinenii, No. 1, pp. 84–88, January–February, 1980.     

Determination of the cystine and cysteine contents of proteins with the aid of an amino acid analyzer

A method is proposed which permits the determination of the amounts of cystine an cysteine in the form pf systeic acid in various proteins of animal and plant origin — even in those in which, on the analysis of standard hydrolysates, the peaks of cysteine are not detected because of their low concentration.Institute of Chemistry, Academy of Sciences of the Tadzhik SSR, Dushanbe. Translated from Khimiya Prirodnykh Soedinenii, No. 4, pp. 551–554, July–August, 1985.     

Demethylenedelpheline — A new diterpene alkaloid from Delphinium corumbosum

Abu Ali Ibn Sina [Avicenna] Tadzhik State Medical Institute, Dushanbe. Institute of the Chemistry of Plant Substances, Uzbek SSR Academy of Sciences, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 1, pp. 50–51, January–February, 1989.     

Synthesis of protected oligopeptides representing fragements of histone fraction HI

Summary Protected oligopeptides corresponding to sequences (31–40) of histone fraction HI of rabbit thymus and (34–41) of histone fraction HI of calf thymus and a number of their analogs have been synthesized.Institute of Molecular Biology, Academy of Sciences of the USSR, Moscow. Institute of Chemistry, Academy of Sciences of the Tadzhik SSR, Dushanbe. Translated from Khimiya Prirodnykh Soedinenii, No. 1, pp. 87–93, January–February, 1977.     

14-Acetylnudicaulidine and 18-deoxylycoctonine — New diterpene alkaloids from Delphinium confusum

Abu Ali Ibn Sina [Avicenna] Tadzhik State Medical Institute, Dushanbe. Institute of the Chemistry of Plant Substances, Uzbek SSR Academy of Sciences, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 1, pp. 48–50, January–February, 1989.     

Isolation and physicochemical characterization of highly polymeric cotton-plant nuclear DNA

A highly polymeric fraction of nuclear DNA has been isolated from two-day shoots of the cotton plant. The DNA has been characterized by the methods of circular dichroism (CD), thermal denaturation, and ultracentrifugation. It has been established that the DNA preparation isolated has a molecular mass of 18·10⁶ daltons, melts at 85.5°C in a fairly narrow interval, and reveals no heterogeneity of its intramolecular nucleotide composition. It has been shown by the CD method that in a solution of the cotton-plant DNA with relatively low ionic strength there is a tendency to the transformation of the B form into the C form.V. I. Nikitin Institute of Chemistry, Academy of Sciences of Tadzhik SSR, Dushanbe, Institute of the Physiology and Biophysics of Plants, Academy of Sciences of the Tadzhik SSR, Dushanbe. Translated from Khimiya Prirodnykh Soedinenii, No. 5, pp. 641–644, September–October, 1984.     

Protein content,activity, and electrophoretic composition of the inhibitor-containing fraction of pea seeds

The amounts of protein and the activities of the proteinase (trypsin) inhibitors of 16 varieties of pea have been studied. The amount of protein ranged from 19.3 to 25.2% and the amidase activities of the trypsin inhibitors from 18 to 40.8 IU. It was found that the electrophoretic spectrum of the inhibitor-containing fraction of the pea seed protein consisted of 12–16 components with molecular masses of 14–89 kDa. Features of the electrophoretic spectrum of some varieties of pea have been established.V. F. Kuprevich Institute of Experimental Botany, Belorussian SSR Academy of Sciences, Minsk. Translated from Khimiya Prirodnykh Soedinenii, No. 2, pp. 243–247, March–April, 1989.     

Reactions of lycoctonine alkaloids containing a 7,8-methylenedioxy group with Na in liquid ammonia

The reactions of 6-dehydroeldelidine and 6-dehydrodelcorine with sodium in liquid ammonia have been studied. Structures have been put forward for the compounds obtained. Features of the fragmentation of these compounds under electron impact are discussed.Abu Ali Ibn Sina [Avicenna] Tadzhik State Medical Institute, Dushanbe. Institute of the Chemistry of Plant Substances, Uzbek SSR Academy of Sciences, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 4, pp. 527–532, July–August, 1989.     

Structure of oxosecodelterine

Periodic acid does not oxidize delterine, an alkaloid fromDelphinium ternatum. However, the further oxidation of 19-oxodelterine, obtained on oxidation by Marion's method, led to oxosecodelterine, which is a semiacetal formed through the C-7 carbonyl group and the C-10 hydroxy group. The treatment of this compound with sulfuric acid led to oxosecodemethanoldelterine.Abu Ali ibn Sina [Avicenna] Tadzhik State Medical Institute, Dushanbe. Institute of the Chemistry of Plant Substances, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 1, pp. 104–107, January–February, 1988.     

Structure of terdeline

A new alkaloid which has been called terdeline has been isolated from the epigeal part ofDelphinium ternatum. Its structure has been demonstrated on the basis of spectral characteristics and the preparation of a demethylene derivative, and also by direct passage from eldelidine to terdeline.Abu Ali Ibn Sina [Avicenna] Tadzhik State Medical Institute, Dushanbe. Institute of the Chemistry of Plant Substances, Uzbek SSR Academy of Sciences, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 3, pp. 396–399, May–June, 1988.     

Histones of the cotton plant. Molecular characteristics of histone H1

A characterization of the histones of two varieties of the cotton plant,Gossypium hirsutum andG. barbadense, has been given with the aid of various electrophoretic systems. Their molecular weights have been determined. An analysis has been made of the cleavage of histone H1 at tyrosine residues. The positive charge and the molecular length of histone H1 have been determined by the method of incomplete succinylation.V. I. Nikitin Institute of Chemistry, Academy of Sciences of the Tadzhik SSR, Dushanbe. Translated from Khimiya Prirodnykh Soedinenii, No. 4, pp. 491–497, July–August, 1986.     

Conformational features of peptide fragments of the C-end of histone H1

The conformational possibilities of four synthesized oligopeptides with the amino acid sequences 165–172, 173–184, 152–172, and 152–184 of the C-end of histone H1 of calf thymus above been studied in solution under various conditions by the method of circular dichroism.V. I. Nikitin Institute of Chemistry, Academy of Sciences of the Tadchik SSR, Dushanbe. Institute of Cytology, Academy of Sciences of the USSR, Leningrad. Translated from Khimiya Prirodnykh Soedinenii, No. 6, pp. 793–798, November–December, 1990.     

Reaction of C19-diterpene alkaloids with sodium in liquid ammonia

The reaction of C₁₉-diterpene alkaloids with sodium and liquid ammonia in the presence and in the absence of a proton donor — an alcohol — has been investigated. In the absence of the proton donor the hydroxy group is converted into a carbonyl group, which is characteristic only for C-6-hydroxy or -acetoxy derivatives.Abu Ali Ibn Sina [Avicenna] Tadzhik State Medical Institute, Dushanbe. Institute of the Chemistry of Plant Substances, Uzbek SSR Academy of Sciences, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 3, pp. 372–375, May–June, 1989.     

Synthesis of the polypeptides (Ala-Orn-Orn)n and (Ala-Orn-Lys)n and their study as models of histones

The regular polypeptides (Ala-Orn-Orn)_n and (Ala-Orn-Lys)_n have been synthesized by the polycondensation of the 2,4,5-trichlorophenyl esters of the corresponding tripeptides, which were obtained by the mixed-anhydride method. Benzyloxycarbonyl and tert-butoxycarbonyl groups were used for blocking the amino groups. The polycondensation was performed in dimethylformamide in the presence of triethylamine at room temperature for 6 days. The benzyloxycarbonyl groups were eliminated from the side chains of the polypeptides with 35% hydrogen bromide in glacial acetic acid. It has been shown by the method of circular dichroism that the adoption of the conformation of an extended left-hand helix is characteristic for both polypeptides, which model the terminal sections of histones.V. I. Nikitin Institute of Chemistry, Academy of Sciences of the Tadzhik SSR, Dushanbe. Institute of Cytology, Adademy of Sciences of the USSR, Leningrad. Translated from Khimiya Prirodnykh Soedinenii, No. 2, pp. 206–211, March–April, 1981.     

Principles of modeling in the study of conformations of histones

A study has been made by the CD method of the conformational potentialities of the polypeptides (Gly-Lys-Gly)_n and (Ala-Lys-Ala)_n and fragments of the terminal sections of histones H4 (the sequence 1–16), H2B (1–21), and H1 (152–184), and they have been used as models of histones in complex formation with DNA under various conditions of the medium.V. I. Nikitin Institute of Chemistry, Tadzhik SSR Academy of Sciences, Dushanbe. Translated from Khimiya Prirodnykh Soedinenii, No. 3, pp. 391–398, May–June, 1989.     

Crystal and molecular structure of 2-hydroxy-5-methylphenyl cyclohexyl ketone

Physicotechnical Institute, Academy of Sciences of the Tadzhik SSR. Institute of Inorganic Chemistry, Siberian Branch, Academy of Sciences of the USSR. Translated from Zhurnal Strukturnoi Khimii, Vol. 31, No. 5, pp. 152–155, September–October, 1990.     

A comparative study of the histones of some varieties of cotton plant

Tetraploid varieties of the cotton plant belonging to the medium- and thin-fibered types have been studied with respect to their histone/DNA ratio, their content of histone fractions, and the amino acid composition of the total histones. It has been shown that these varieties differ with respect to their contents of the lysine-rich histone fraction Hl, and also by the amounts of certain amino acids in the total histone.V. I. Nikitin Institute of Chemistry, Academy of Sciences of the TadzhSSR, Dushanbe. Translated from Khimiya Prirodnykh Soedinenii, No. 1, pp. 99–102, January–February, 1985.