双波长分光光度法研究——直线法同时确定K系数及工作曲线斜率

本文根据一组不同组成的已知混合溶液的吸光度,采用线性回归方法同时确定双波长K系数法的系数K及工作曲线斜率,此法可适用于吸光度加和性差的显色体系,对于加和性较好的体系,测定精度亦能进一步提高。     

比值导教光谱法同时测定苯酚间苯二酚对苯二酚

以比值导数光谱法对苯酚、间苯二酚、对苯二酚的二组分和三组分混合体系进行了分析。方法以混合物的波谱除以干扰组分的波谱得到比值波谱,再用“减(加)法技术”以比值波谱对波长求导得到比值导数波谱,方法兼具导数光谱法能消除低频背景和高频噪声干扰的优点。由此得到的比值导数波谱可完全消除干扰组分的吸光度的贡献。此法可很方便对二组分体系进行测定。进一步以求得的比导数波谱对波长二次求导,可对三组分体系进行分析,用此法对以上的二组分和三组混合体系进行分析均得到较好结果。     

比值导数光谱法同时测定苯酚间苯二酚对苯二酚

以比值导数光谱法对苯酚、间苯二酚、对苯二酚的二组分和三组分混合体系进行了分析。方法以混合物的波谱除以干扰组分的波普得到比值波谱,再用“减（加）法技术”以比值波谱对波长求导得到比值导数波谱,方法兼具导数光谱能消除低频背景和高频噪声干扰的优点。由此得到的幽会导数波谱可完全消除干扰组分的吸光度的贡献,此法可很方便地对二组分体系进行测定。进一步以求得的比导数波谱对波长二次求导,可对三组分体系进行分析,用此     

多波长K系数法的改进研究

本文提出了测定混合干扰组分改进的多波长Ｋ系数法。首先采用参比波长组及测量波长组，组中各波长以吸光度的和参与Ｋ系数和△Ａ的计算，以减少测量误差；其次Ｋ系数的测定在含有所有干扰组分的溶液中进行，以减少组分吸光度非严格１００％加和引起的误差，Ｋ系数的求解使用算术平均值法，而不用量小二乘法，以减少计算误差。     

计算机差谱法测定桔子粉中混合色素含量

将红外光谱分析中“独立峰”差谱法应用于可见光区混合物谱峰的分离和测定。数据用微机处理。结果表明:此方法对于吸光度具有加合性的混合体系中单一组分含量的测定,准确度良好。对桔子粉中柠檬黄、胭脂红两种色素联合测定,其回收率分别为93.1％～99.3％和99.1％～112.6％。相对标准偏差分别为1.25％和0.00％。     

吸光度比值-导数光谱法同时测定苯酚和间苯二酚

吸光度比值-导数光谱是近年来提出的多组分光谱分析法.该法以二元混合物的混合光谱与其中某一组分(被视为干扰组分)的标准光谱的比值对波长求导,从而得到吸光度比值-导数光谱.由于干扰组分对吸光度比值-导数光谱的贡献为零,所以可利用吸光度比值-导数值测出待测组分的含量.本文在普通分光光度计上用计算法完成了吸光度比值导数运算,同时测定了复方雷琐辛涂剂中苯酚(P)和间苯二酚(R)的含量,取得了较为满意的结果.     

PLS–分光光度法同时测定三组分阳离子染料

将偏最小二乘法用于可见分光光度分析,建立一种同时测定阳离子黄X–8GL、阳离子红X–FG、阳离子艳蓝RL三组分混合染料含量的新方法。在380～780 nm范围内,将测定的26组混合溶液的吸光度值作为校正集,另8组混合溶液的吸光度值作为预测集,结合二阶差分法确定最佳主成分数。测得三组分混合体系中校正集的相关系数分别为0.9988,0.9994,0.9964;交互验证均方根误差(RMSECV)分别为0.0754,0.1852,0.2168;预测集的相关系数分别为0.9984,0.9996,0.9981;预测均方根误差(RMSEP)分别为0.1086,0.1877,0.2515。该方法无需样品分离,可为染色过程中多组分染料浓度的在线监测提供技术支撑。     

三波长线性回归分光光度法同时测定解热镇痛药物中的各组份

1引言本文结合三波长法和K系数法的基本原理，使在适宜的测量波长组合下，与待测组分的浓度成线性关系．本文依据一组含有不同比例待测和干扰组份的标准混合溶液的吸光度值，采用一元线性回归方法处理，在选择各组份最佳测定波长组合的同时建立其浓度计算模型．该法可直接测定混合体系中某一组份或各组份，方法简便实用。2方法原理根据三波长分光光度法原理，在适宜的测量波长组合下，混合多组份体系中的某一组份在其测定波长和处的吸光度值与该组份的浓度之间的关系为：式（4）为一直线方程，其斜率为K，截距则为系数k。为减少误差，获得…     

因子分析-氢化物发生原子吸收法分析砷的形态

报道了用流动注射氢化物发生原子吸收法（ＦＩ－ＨＧＡＡＳ）对砷４种形态,Ａｓ,ＤＭＡ,ＭＭＡ）的混合体系进行测量后,根据吸光度加和性原则,用因子分析法（ＴＦＡ）对测定值进行解析,从而测定了混合体系中砷存在形态的数目、种类和含量。实验结果表明,不同酸度介质中不同形态砷校正曲线的斜率可作为相应的目标检测向量,应用于μｇ／Ｌ级低浓度砷的形态分析,平均回收率为９３．９％,结果令人满意。     

比值导数波谱法分析食品中混合色素

利用混合物的波谱除以干扰组分的波谱得比值波谱,对波长求导得比值导数波谱,由此可消除干扰组分的吸光度叠加效应。该法能有效地对三组分混合体系进行分析。利用此法对亮蓝、日落黄、胭脂及柠檬黄等色素所组成的二组分和三组分混合体系进行分析,取得满意效果。     

血红蛋白作为过氧化物模拟酶催化显色体系的研究与应用

研究了以血红蛋白（Ｈｅｍｏｇｌｏｂｉｎ,Ｈｂ）作为过氧化物模拟酶对过氧化氢－４－氨基安替比林（４－Ａｍｉｎｏａｎｔｉｐｙｒｉｎｅ,４－ＡＡＰ）氯取代苯酚衍生物显色体系的催化反应性能,探讨了不同氯取代苯酚类衍生物作为酶催化反应氢供体底物的构效关系及酶催化反应的可能机理。拟定了Ｈｂ催化Ｈ２Ｏ２氧化４－ＡＡＰ－２,３,个三氯苯酚（２,３,４－Ｔｒｉｃｈｌｏｒｏｐｈｅｎｏｌ,ＴＣＰ）显色体系用于Ｈ２Ｏ２的测定方法。该方法测定Ｈ２Ｏ２灵敏度高,表观摩尔吸光系数为 ２．２１×１０４ Ｌ·ｍｏｌ－１·ｃｍ－１。将拟定方法与葡萄糖氧化酶催化反应偶联,用于人血清样品中葡萄糖含量的测定,得到满意的结果。     

Raman spectroscopic investigation of film thickness

The determination of film thickness is of prime importance in the quality assurance of coated pharmaceutical preparations. The rapid measurement of this parameter is problematic for multi-particulate pellet systems. The aim of this study was to apply the Raman spectroscopic method for the determination of the thickness of polymer coating on pellets. The change of Raman intensity was compared with measured film thickness, which was calculated from the change of the geometric parameters of the pellets, measured with an image-analyzing system. The results revealed that despite some difficulties Raman spectroscopy is a suitable method for the fast and accurate determination of film thickness on multi-particulate systems.     

非线性主成分回归分光光度法同时测定复方制剂中两组分的含量

构造多组分体系的非线性模型,并用主成分回归法来解析,建立了同时测定两组分的非线性主成分回归分光光度法,方法用于复方苯甲酸制剂中苯甲酸和水杨酸含量的测定,结果优于线性主成分回归分光光度法。     

Calculation of wetting angles in crude oil/water/quartz systems

A method for the determination of water-advancing wetting angles has been developed and tested. The method allows measurements in black oils, as opposed to traditional techniques which substitute transparent model oils prior to measurements. The method is based on the Laplace equation and axisymmetric drop shape analysis. The main source of error is the determination of the drop volume. Results in transparent systems are comparable to results using other techniques. Wetting angles are determined for water in two different crude oil systems, using quartz as the substrate. The quartz surfaces are water wet over large pH ranges, but it is possible to accurately identify pH intervals where the surfaces are intermediate or oil wet.     

Chain transfer in ethylene polymerization. III. An improved method of calculating polymerization chain-transfer constants

The method of determining chain-transfer constants in polymer systems, originally developed by Mayo, has been extended to the simultaneous determination of constants for several transfer agents. The validity of various calculation methods was examined. Particular attention was devoted to the question of how precisely chain-transfer constants are known. This extended method was applied to the determination of chain-transfer constants in ethylene polymerization under conditions where the limiting molecular weight in the absence of transfer agents cannot be directly measured, and it was shown that precision is improved (uncertanty reduced) by simultaneously determining more than one transfer constant.     

某些分子光谱分析法测定蛋白质的进展

评述包括吸光光度法、荧光光度法及新近发展起来的共振光散射法等分子光谱分析方法定量测定蛋白质的某些进展。表列了重要的反应体系及分析特征。引用文献97种。     

Polarographie des periodates applications analytiques

The polarography of periodates is described and that of hypoiodites is outlined. The first is applied to the determination of traces of iodates in periodates and conversely; in addition, it allows the micro determination of potassium by the method of willard and BOYLE to be adapted to the polarograph, giving a rapid and sensitive method. Possible applications of the second to the determination of hypoiodites in solution and to similar unstable systems in general are given.     

多元光度测定病态系统的岭回归估计

考察了含多种酚类的有机混合体系的同时测定，发现对于这类化学与光谱性质皆十分相近的混合物体系，最小二乘法和卡尔曼滤波方法都不能给出令人满意的结果，甚至出现负值，而脾岭回归估计方法对此类病态体系进行分析，所得结果明显优于最小二乘法与卡尔曼滤波方法。     

Batch and Flow Injection Fluorimetric Determination of Fluoxetine

Abstract

A method for the fluorimetric determination of fluoxetine in continuous and discontinuous systems is reported. The method is based on the hydrolysis of fluoxetine in acid medium. The fluorescent product has a spectrum with excitation and emission maxima at 253 and 306 nm, respectively. The method was applied to the determination of fluoxetine in pharmaceutical products.     

双波长双指示剂催化光度法测定铜的研究

研究了在pH 4.0的HAc～NaAc缓冲介质中,利用痕量铜(Ⅱ)催化过氧化氢氧化罗丹明B和亚甲基蓝褪色的指示反应,通过测量540 nm和660 nm下,催化体系和非催化体系吸光度的变化,建立了双波长双指示剂催化动力学光度法测定痕量铜的新方法.方法的线性范围为0.00080～0.048μg/mL,检出限为4.0×10~(-11) g/mL.方法可用于水中铜的测定.