聚合物AQ—二甲基二茂铁修饰微组合电极的电化学行为及葡萄糖传感器的研究

本文用聚合物ＡＱ将二甲基二茂铁、葡萄糖氧化酶固定在微组合电极上制成了葡萄糖微传感器。二甲基二茂铁作为一个介体将电子从电极传递给葡萄糖氧化酶（ＧＯＤ）。催化电流与葡萄糖浓度在１～１２ｍｍｏｌ／Ｌ呈线性关系。探讨了温度、ｐＨ对催化电流的影响。微传感器的响应时间小于１５秒，ＡＱ膜的阳离子交换特性和很强的附着力以及二甲基二茂铁的工作电位低，使微传感器的稳定性有所提高，并且消除了抗坏血酸、尿酸的干扰。血清中     

二茂铁及其衍生物作为电化学传感器的研究进展

本文对近二十年来国内外对二茂铁及其衍生物作为电化学传感器的研究进展做了较全面的综述。主要讨论了二茂铁及其衍生物修饰电极的制备、表征,介绍了二茂铁及其衍生物作为电化学传感器的应用情况,并探讨了今后的研究方向。     

Nafion-二茂铁-双酶修饰的葡萄糖传感器

用二茂铁作为过氧化物酶与玻碳电极的电子传递体,通过牛血清白蛋白-戊二醛交联剂把葡萄糖氧化酶和过氧化物酶固定在Nafion-二茂铁修饰玻碳电极上,制备成葡萄糖传感器。由于工作电位低,电活性物质如抗坏血酸、尿酸等对测定无干扰。该传感器的线性范围为5.0×10～(-4)～2.5×10～(-2)mol/L,响应时间小于30s.     

基于纳米MnO_2二茂铁修饰丝网印刷碳电极的尿酸生物传感器研究

在丝网印刷碳电极上修饰纳米MnO2,并利用戊二醛和β-环糊精交联固定尿酸酶,以二茂铁作为电子媒介体,研制用于测定尿酸浓度的生物传感器.实验结果表明,纳米MnO2降低了电子媒介体二茂铁的氧化还原反应电位,且纳米MnO2与电子媒介体二茂铁在尿酸生物传感器中表现出协同增效效应.该尿酸生物传感器线性响应范围是6.0×10-6～1.2×10-3 mol/L,检出限为3.0×10-6 mol/L.用纳米MnO2修饰酶电极,改善了电极表面条件,加快了电极反应速率,提高了尿酸传感器的灵敏度.     

二茂铁-Nafion修饰葡萄糖传感器的研究

1 引言 第二代电流式酶传感器采用非生理的氧化还原介体如二茂铁及其衍生物、铁氰化钾、四硫富瓦烯、醌及其衍生物、钉化合物及麦尔多蓝等代替氧,起着酶与电极之间的电子传递作用,克服了第一代电流式酶传感器背景电流大,响应特性差,易受环境中氧浓度的影响,干扰大等缺点。但目前第二代电流式酶电极还存在着介体流失,电极污染及一些电活性物质干扰等问题。商品化的Nafion甲醇溶液不易溶解酶且能使部分酶失活,本文把Nafion甲醇溶液制成Nafion水溶液,将葡萄糖氧化酶溶解在Nafion水溶液,然后把它修饰在修饰有二茂铁的玻碳电极的表面,最后再修饰一层Nafion膜,成功地制备了二茂铁-Nafion修饰葡萄糖传感器,Nafion膜不仅能把抗坏血酸、尿酸等电活性物质阻挡在电极外,防止其干扰,同时具有防污性能,而且能防止二茂铁的流失,提高电极的稳定性。该传感器具有抗干扰能力强,响应快等特点。     

离子结合物在二茂铁介体电流型葡萄糖生物传感器中的应用研究

二茂铁及其衍生物由于氧化还原可逆性好,不溶于水,因此可利用其作介体电流型生物传感器的电子转移介体。但该类传感器的重现性、稳定性差。本文首次提出以含(C_6H_5)_4·(CH_3)_4离子缔合物的碳糊电极为基础电极,以二茂铁(FeCp_2)为电子转移介体制成葡萄糖生物传感器。该传感器重现性、稳定性好,响应迅速,测量准确度高。     

一次性唾液α-淀粉酶生物传感器的研制

基于丝网印刷技术在PVC薄膜上制备了一次性碳电极,用Nafion固定二茂铁(Fc)作为电子介体,将α-糖苷酶和葡萄糖氧化酶(GOD)滴加于二茂铁修饰的电极上,滴加明胶晾干,戊二醛间接交联固定制成一次性唾液α-淀粉酶生物传感器,用计时电流法测定对α-淀粉酶的响应.实验结果表明,该传感器响应电流与α-淀粉酶活性在60～840 U/L之间呈现良好的线性关系,检出限为17 U/L.该生物传感器响应时间短,达到95%稳态响应时间不超过30 s,具有良好的一致性、准确性和稳定性.探讨了pH、缓冲液、温度及其它干扰物质等对该传感器的影响.该传感器可用于唾液α-淀粉酶浓度的快速、准确检测.     

主客体葡萄糖、乳糖生物传感器的研制

以β－环糊精与戊二醛缩合而成的聚合物（β－CDP）为主体，二茂铁为客体，使之形成稳定的包埋络合物，以此包络物中的二茂铁为电子传递介体，制成了葡萄糖，乳糖生物传感器，由于包络物的形成，避免了电子传递介体的流失，生物传感器的稳定性得到提高，使用寿命得到延长。     

基于微带阵列电极的微型葡萄糖传感器研究

用微电子薄膜技术制作了微带阵列电极（ＭＡＥ）,考察了该电极在铁氰化钾,过氧化氢溶液中的电化学行为。在微带阵列电极表面,修饰一层全氟代磺酸酯膜作为基底电极,并把电子介体二茂铁及葡萄糖氧化酶固定在基底电极上制备了微型葡萄糖传感器,探讨了微酶电极对葡萄糖氧化过程的催化作用。该微酶电极响应时间小于１０ｓ,检测线性上限为８ｍｍｏｌ／Ｌ。     

基于双二茂铁和β-环糊精之间主客体识别模式信号放大的均相电化学适体传感器用于凝血酶的灵敏检测

合成了双二茂铁化合物并用于修饰在凝血酶适配体(TBA)的两端作为电化学信号标记物,构建了一款基于双二茂铁与β-环糊精(β-CD)之间主客体识别原理进行信号扩增的均相电化学凝血酶传感器。当电化学TBA探针与凝血酶发生特异性结合后,TBA探针由原来的茎环结构变成"G-四链体",双二茂铁分子通过主客体识别作用进入修饰在金电极表面的β-CD的空腔内,产生了稳定的电化学电流响应信号。该凝血酶电化学均相传感器在0.02～62.5 nmol/L范围内对凝血酶呈良好的线性关系,检出限为8.4 pmol/L。该传感器对凝血酶可为凝血酶的快速检测提供了一个可行的方案。     

表面等离子体共振生物传感器及其应用

表面等离子体共振(Surface Plasmon Resonance,SPR)生物传感器在检测生物分子特异性结合方面具有的高灵敏度、免标记及检测快速等优点,使其在过去的20年中在生命科学、医药科学、食品安全等领域取得了快速发展。本文对SPR生物传感器进行了简要介绍,并着重对其在生命科学、医药学、环境分析、食品安全等领域的应用进行了综述,最后对该领域的研究前景进行了展望。     

Derivatography A complex method in thermal analysis

This review gives a survey of the theoretical considerations which led to the introduction of derivative methods in thennoanalysis, and of the development of complex thennoanalytical methods. The paper introduces the method of derivatography and its development, and surveys the wide field of its application in chemistry, technology, physical chemistry and biology.     

Trendwatcher or trendsetter; 50 years of MTAA

The development on neutron activation analysis (NAA) into a technique of practical interest effectively started about 60 years ago, when nuclear reactors became available and widely accessible as intense sources of neutrons. During 50 out these 60 years, the series of Modern Trends in Activation Analysis (MTAA) Conferences acted as a true companion and facilitator of this growth. As trendwatcher they signalized the many initiatives that contributed to the development of activation analysis and its applications. A period has come to an end of impressive development resulting from sometimes revolutionary changes in radiation detection and data processing, and much improved irradiation facilities, NAA has reached a full stage of development, with emphasis on routine application and with remaining developments of in marginal impact. NAA is being challenged increasingly in the last 30 years by alternative techniques for multi trace element analysis. The MTAA Conference and with it the ICAA, the International Committee on Activation Analysis, can play an important and active role in this process of identifying and selecting key areas, and even promoting concerted action in those areas. Such an evolution of focus from retrospective to prospective, from trendwatcher to trendsetter, may well allow the MTAA Conference to continue and even expand its role in future development of NAA and its applications. The ideas about the future of the MTAA Conferences and its organization are elaborated upon and some possible subjects for focused development activities are indicated.     

Development of Chemical Probes for Functional Analysis of Anticancer Saponin OSW‐1

Chemical probe‐based approaches have proven powerful in recent years in the target identification studies of natural products. OSW‐1 is a saponin class of natural products with highly potent and selective cytotoxicity against various cancer cell lines. Understanding its mechanism of action is important for the development of anticancer drugs with potentially novel target pathways. This account reviews recent progress in the development of OSW‐1 derived probes for exploring the mechanism of its action. The key to the probe development is a judicious choice of functionalization sites and a selective functionalization strategy. The types of probes include fluorescent probes for cellular imaging analysis and affinity probes for target identification analysis.     

克拉霉素的合成进展

克拉霉素属大环内酯类抗生素，是第二代红霉素产品，其抗菌谱广，抗菌活性 高，药代动力性能好，在国内外受到广泛重视，有着极其重要的应用和发展前景。 对克拉霉素研究的起源与发展、研制历程、合成过程中6－羟基甲基化选择性、活 泼基团的保护与脱保护、各种克拉霉素合成工艺路线以及最新合成方法进行了较为 详细的综述。     

超级电容电池

本文比较了超级电容器、锂离子电池和超级电容电池的结构、原理、研究现状和发展前景。超级电容电池的正极具有超级电容器电极的结构和双电层储能机制,负极具有类似锂离子电池负极的结构和快速电化学储能机制。超级电容器和锂离子电池的发展空间都很有限,而作为两者结合的产物的超级电容电池可兼具高比功率、高比能量、高放电电压和长循环寿命的优点,是未来储能电池的发展方向之一,但还面临缺乏具有高分解电压的电解液和高充电电压下电解液中离子枯竭的问题。     

Aurora Kinase B Inhibition: A Potential Therapeutic Strategy for Cancer

Aurora kinase B (AURKB) is a mitotic serine/threonine protein kinase that belongs to the aurora kinase family along with aurora kinase A (AURKA) and aurora kinase C (AURKC). AURKB is a member of the chromosomal passenger protein complex and plays a role in cell cycle progression. Deregulation of AURKB is observed in several tumors and its overexpression is frequently linked to tumor cell invasion, metastasis and drug resistance. AURKB has emerged as an attractive drug target leading to the development of small molecule inhibitors. This review summarizes recent findings pertaining to the role of AURKB in tumor development, therapy related drug resistance, and its inhibition as a potential therapeutic strategy for cancer. We discuss AURKB inhibitors that are in preclinical and clinical development and combination studies of AURKB inhibition with other therapeutic strategies.     

The Cambridge Structural Database in Retrospect and Prospect

The Cambridge Crystallographic Data Centre (CCDC) was established in 1965 to record numerical, chemical and bibliographic data relating to published organic and metal–organic crystal structures. The Cambridge Structural Database (CSD) now stores data for nearly 700 000 structures and is a comprehensive and fully retrospective historical archive of small‐molecule crystallography. Nearly 40 000 new structures are added each year. As X‐ray crystallography celebrates its centenary as a subject, and the CCDC approaches its own 50th year, this article traces the origins of the CCDC as a publicly funded organization and its onward development into a self‐financing charitable institution. Principally, however, we describe the growth of the CSD and its extensive associated software system, and summarize its impact and value as a basis for research in structural chemistry, materials science and the life sciences, including drug discovery and drug development. Finally, the article considers the CCDC’s funding model in relation to open access and open data paradigms.     

C60系列结构,制备分离方法与性质的研究进展

本文综述地评述了Ｃ６０的结构，制备分离方法，及其衍生物表现出的异常的物理、化学性质，其碱金属盐呈现导电性或超导性、铁磁性、光学非线性、耐磨擦性能等。为进一步研究Ｃ６０及其衍生物提供了信息。     

Selective Monitoring of the Enzymatic Activity of the Tumor Suppressor Fhit

Cancer is a leading cause of death worldwide. Functional inactivation of tumor suppressor proteins, mainly by mutations in the corresponding genes, is a key event in cancer development. The fragile histidine triade protein (Fhit) is a tumor suppressor that is frequently affected in different cancer types. Fhit possesses diadenosine triphosphate hydrolase activity, but although reduction of its enzymatic activity appears to be important for exerting its tumor suppressor function, the regulation of Fhit activity is poorly understood. Here, we introduce a novel fluorogenic probe that is suited to selectively analyze the enzymatic activity of Fhit in extracts derived from human cells. This novel method will allow in‐depth insight into the mechanisms involved in Fhit regulation in biologically relevant setups and, thus, into its role in the development of cancer.