冠心病患者全血微量元素锌、铜、铁的分析

用原子吸收法测定５０例健康人、５２例冠心病患者全血微量元素锌、铜、铁的含量，并对５２例冠心病患者全血微量元素锌、铜、铁的含量及锌／铜比值，与其血脂总胆固醇（ＣＨ）、甘油三酯（ＴＧ）、高密度脂蛋白（ＨＤＬ）、低密度脂蛋白（ＬＤＬ）的浓度分别作线性相关分析。结果表明：（１）冠心病患者全血锌、铁、锌／铜比值均高于健康人（Ｐ＜０．０５），全血铜低于健康人（Ｐ＜０．０５）。（２）全血锌与ＨＤＬ呈负相关（Ｐ＜０．０１）、锌／铜比值与总胆固醇呈正相关（Ｐ＜０．０５），铁与ＬＤＬ呈正相关（Ｐ＜０．０１）．提示锌／铜比值增高及体内铁储存增加均可能是冠心病易患因素．     

小儿甲型肝炎早期血清锌、铜、铁、镁含量变化的探讨

通过对９８例甲型肝炎早期住院患儿血清锌、铜、铁、镁含量的检测，显示在小儿甲型肝炎早期，血清锌、镁含量与对照组无显著差异（Ｐ＞０．０５），血清铜、铁含量明显增高，与对照组存在非常显著差异（Ｐ＜０．０１）．文章对甲型肝炎患儿血清锌、铜、铁含量的变化和临床意义进行了探讨．     

中山地区厌食儿与发锌,铁,铜,钙的相关性临床研究——附387例分析

对中山地区厌食儿２２２例的发锌、铁、铜、钙含量进行测定，并与１６５例健康儿对照分析。结果表明：厌食儿组发锌均值比健康儿组明显偏低，铜／锌均值明显偏高，Ｐ均〈０．０１；发钙偏低，Ｐ〈０．０５；发铁、发铜无明显差异，Ｐ〉０．０５。厌食儿组发锌下降率为９６．３６％，健康儿组为１３．３３％，两组间存在高度显著性差异，Ｐ〈０．０１。说明厌食儿体内微量元素变化以锌最明显。另外对２２２例厌食儿中的１５１例缺锌儿     

通脉降脂灵对实验性小白鼠虚证模型血清锌,铜浓度的影响

研究了通脉降脂灵对实验性小白鼠虚证模型血清锌、铜浓度的影响。结果表明，肌注氢化考的松１．０ｍｇ／只鼠，从第２￣３天起，可出现阴虚证候。血清锌同对照组相比较，明显降低３倍，铜及铜锌比值亦下降，Ｐ〈０．０１。而预先服用通脉降脂灵生药或提取物者，氢化考的松不能复制出阴虚证模型，血清锌、铜浓度及铜锌比值与对照鼠无显著差异，Ｐ〉０．０５。说明通脉降脂灵生药及提取物具有较强防治阴虚证的作用。可用于高血脂的防治     

糖尿病患者血清中微量元素分析

对２０例糖尿病患者血清锌、铁、锰、钙、铜含量检测发现，糖尿病组较对照组血清铁、铜高，差异显著（Ｐ〈０．０５），血清锌、锰略低（Ｐ〉０．０５），血清钙略高（Ｐ〉０．０５）。表明糖尿病可导致微量元素代谢紊乱，在治疗糖尿病时要适当补充微量元素；同时微量元素含量可作为糖尿病的一个控制指标。     

脊髓小脑变性疾病病人血清铜,锌,铝,铁含量的研究

脊髓小脑变性疾病（ＳＣＤ）的病因可能与遗传、神经生化紊乱、感染、微量元素平衡障碍及自由基损伤等因素有关，其中微量元素平衡障碍的研究具有重要意义。检测了ＳＣＤ中发病率较高的橄榄桥小脑萎缩症（ＯＰＣＡ）及晚发性小脑皮质萎缩症（ＬＣＣＡ）病人１５例血清中铜、锌、铝、铁等微量元素含量。发现病人比对照组铜明显降低，Ｐ〈０．０１；锌明显降低，Ｐ〈０．０１；铝明显升高，Ｐ〈０．０１；铁无明显差别。     

复发口疮治疗前后血清SOD,MDA等改变研究

选取了３５例ＲＡＵ患者与３０例正常对照，作锌、铁制剂辅助中草药雷公藤、丹参片治疗前后血清ＳＯＤ、ＭＤＡ的改变，实验组与对照组有显著性差异（Ｐ〈０．０１），治疗前后对比又有显著性差异（Ｐ〈０．０１）。而ＰＥＧ－ＣＩＣ的改变，实验组比对照组有显著性增高（Ｐ〈０．０１），治疗前后对比又有显著性差异（Ｐ〈０．０１）。     

糖尿病人血清与尿中微量元素变化与营养保健的探讨

通过对１１７例糖尿病人血清及尿液分析：发现血清中铜、铁、锌、镁四元素明显低于正常人对照组，差异极为显著（Ｐ＜０．００１）；尿中２４小时排出总量除铁元素无差异外，其他三元素都明显高于正常人对照组（Ｐ＜０．００１）。铜、锌的排出总量是正常人２倍以上，伴随糖尿病增加重随之进一步增加，而血清中铜、锌的含量却随之进一步减少，呈负相关趋势．进而又加重了体内微量元素的缺乏．铜、锌是人体多种酶数的辅基成份或激活因子，对丘脑至靶器官等各级内分泌组组分泌激素有着重要的作用．在常规治疗中补充铜、锌营养对促进糖尿病患者的康复有重要的作用．     

恶性骨肿瘤病人血清铜,锌测定

对１９名恶性骨肿瘤病人进行了血清铜、锌测定，与正常对照组进行比较，结果显示患病组血清铜２１．２±６．４４μｍｏｌ／Ｌ高于对照组的１４．２±３．６０μｍｏｌ／Ｌ（Ｐ＜０．０１），且铜／锌比值明显增高（Ｐ＜０．０１）。血清锌两组间差异无显著性意义。     

口腔粘膜免疫性疾病患者血清微量元素变化研究

选择了４５例免疫性口腔粘膜病人进行血清微量元素含量变化研究，以探讨日腔粘膜免疫性疾病患者血清微量元素的变化，给临床提供使用微量元素作辅助治疗的实验依据。结果显示血清锌、铜含量均显著低于对照参考值（Ｐ＜０．０１ｆ）．铁含量低于参考值（０．０１＜Ｐ＜０．０５），而血清镁含量高于对照参考值（Ｐ＜０．００１）。     

母血、脐血、母乳锌、铁、硒水平配对分析

目的:了解母血、脐血、母乳之间锌、铁、硒含量的相关性。方法:对5 6例正常产妇进行了母血、脐血、母乳锌、铁、硒含量的配对分析。结果:各样本中锌在母乳中的含量比脐血中高,其差异具有显著性,P <0. 0 5 ;锌在脐血中的含量显著高于母血中,P <0 . 0 1 ;铁在脐血中含量明显高于母血,而母血又明显高于母乳,P <0 . 0 1 ;脐血、母血、母乳硒含量依次升高,其间差异不明显;母血与脐血中的锌含量具有明显的相关性(r =0 .478,P <0 .0 1 ) ,铁也具有相似的变化(r=0 . 5 2 2 ,P <0 . 0 1 ) ;母血与母乳中的锌、铁也具有相关性(r =0. 3 5 6) ,P <0 . 0 5 ;r =0. 42 1 ,P <0 . 0 1 )。结论:锌、铁、硒为胎儿生长发育中的重要元素,在母乳中含有大量的锌和硒,故应提倡母乳喂养,但4个月后应添加含铁辅食,补充母乳中铁的不足。     

胎儿脐带血铅水平与钙、锌、铁的关系研究

目的研究胎儿脐带血中的铅与钙、锌、铁的含量以及两两之间的相关性。方法采用原子吸收光谱法对398例脐带血铅和钙、锌、铁的含量进行测定,并对它们进行相关性分析。结果 398例脐带血铅和钙、锌、铁的质量浓度分别为铅(37.96±17.78)μg/L、钙(1.51±0.29)mmol/L、锌(30.01±11.14)μmol/L、铁(8.63±1.39)mmol/L。线性回归分析显示:铅与钙(r=-0.563,P0.01)、铅与锌(r=-0.424,P0.01)有相关性,铅与铁无显著相关(r=0.018,P0.05)。结论随着胎儿体内血铅水平的上升,二价元素钙、锌含量有下降趋势,即中毒元素铅会干扰胎儿体内的必需二价元素的代谢。     

130名哈萨克族牧民血清铁、血红蛋白含量与高血压关系的分析

为探讨乌鲁木齐南山130名哈萨克族牧民血清铁及血红蛋白含量与原发性高血压的关系,给哈萨克族原发性高血压的病因学研究提供科学依据,对这些牧民进行了血压测定和血清铁、血红蛋白含量分析。结果表明,130名哈萨克族牧民高血压检出率为53.3%;高血压组血清铁水平明显高于正常组(P=0.000);高血压组血红蛋白含量也明显高于正常组(P=0.004);进一步分析表明,高血压组男性和女性血清铁水平明显高于正常组男性和女性(P0.01和P0.001);血红蛋白含量高血压组女性较正常血压组女性高,差异有统计学意义(P0.05),而男性两组差异不明显。提示哈萨克族高血压患者血清铁和血红蛋白的含量与高血压的发生可能有关。     

Determination of Serum Iron and Iron Binding Capacity Using 4-(2-Pyridylazo) Resorcinol (Par)

Abstract

Procedures are described for the determination of serum iron and iron binding capacity using the chromogenic reagent 4-(2-pyridylazo) resorcinol (PAR). The high molar absorptivity of the iron (II) PAR complex makes possible an approximate two fold increase in sensitivity over previous methods. The described procedures have few interferences, and give linear results for samples containing 0 – 750 μg/dl of iron.     

Minimalism approach for determination of Cu, Fe and Zn in serum by simultaneous electrothermal atomic absorption spectrometry

Copper, iron and zinc were determined in serum by simultaneous atomic absorption spectrometry (SIMAAS). The minimalism approach was adopted throughout this analytical method, to reduce time, costs, sample, reagent, energy requirements, and residue production. Samples were 80-fold diluted with 0.01% (w/v) Triton X-100+1% (v/v) HNO₃ directly in the autosampler cups. Three strategies were implemented to match the final diluted analyte concentrations with the SIMAAS linear concentration ranges: a reduced 5 μl aliquot of analytical reference or diluted sample solution was introduced into the preheated graphite tube at 100 °C; a super-estimated pyrolysis temperature was chosen for selective zinc volatilization; and a mini argon flow of 50 ml min⁻¹ was used during the atomization step. The pyrolysis and atomization temperatures for the simultaneous heating program were 700 and 2300 °C, respectively. The characteristic masses for copper (26 pg), iron (16 pg), and zinc (2.7 pg) were estimated from the analytical graphs. The detection limits (n=20, 3σ) were 4.0, 2.2, and 0.4 μg l⁻¹ for copper, iron and zinc, respectively. The reliability of the entire procedure was checked with the analysis of Seronorm™ trace elements in serum (Sero AS). Serum samples of five volunteers were analyzed and the recovery tests for additions of 2.0, 2.0 and 1.0 mg l⁻¹ were 100±4, 99±6, and 95±5% for copper, iron and zinc, respectively.     

低锌小儿的血红蛋白与微量元素锌、铁相互作用的探讨

通过对缺锌小儿服用锌剂和铁剂后发锌和外周血血红蛋白变化,研究了锌剂与铁剂在体内的相互作用。结果表明,低锌小儿贫血发生率与健康儿童无异,常规锌剂治疗对血红蛋白无明显影响。提示缺锌小儿合并贫血时可同时用常规剂量的锌剂和铁剂治疗,无锌、铁在体内相互拮抗问题。     

甲状腺功能亢进症患者血清铜、锌、铁、钙、镁的研究

为研究甲状腺功能亢进症患者血清中 5种微量元素的变化及其与甲状腺功能激素间的关系 ,用分光光度分析和放射免疫分析分别检测了 1 2 2例甲状腺疾病患者血清中微量元素Cu、Zn、Fe、Ca、Mg含量和血清中甲状腺激素 (T3、T4 )水平。结果表明 ,甲状腺功能亢进症患者血清中Cu、Ca元素水平明显高于对照组 (P <0 0 1 ) ,Zn元素水平明显低于对照组 (P <0 0 0 1 ) ,Fe、Mg元素两组比较无统计学意义 ;血清Cu与T3、T4 浓度呈现一定程度的正相关 ,提示微量元素在甲状腺激素的合成、代谢和生物作用中可能有重要意义 ,甲状腺功能亢进症患者过多释放甲状腺素导致血清中元素的异常变化 ,此变化既能反映病人机体代谢紊乱程度 ,同时也可作为病情控制的参考指标。     

Influence of heavy metals, especially lead, on lipid metabolism, serum alpha-tocopherol level, total antioxidant status, and erythrocyte redox status of copper smelter workers

An assessment of influence of the occupational exposure to heavy metals, especially lead, on serum lipids (including lipid peroxides), total antioxidant status, erythrocyte redox status, and serum alpha-tocopherol level was performed in a group of 141 healthy male copper smelter workers. The following parameters were measured: blood lead and cadmium levels, serum manganese, copper, zinc, calcium and magnesium levels, free erythrocyte protoporphyrins (FEP), total cholesterol, HDL₂-, HDL₃-cholesterol, triglycerides and lipid peroxides in serum, erythrocyte superoxide dismutase (SOD_E), catalase (Cat_E) and glutathion peroxidase (PxGSH_E) activities, erythrocyte reduced glutathione level (GSH_E), serum alpha-tocopherol level, and serum total antioxidant status (TAS). Mean Pb_B was within the norm range (328.2 ± 141.7 μg/L), but mean Mn_S concentration slightly exceeded 10 μg/L (11.04 ± 3.79 μg/L). Mean cholesterol and triglycerides concentrations were near the highest borderline values. We found a significantly negative correlation between lead levels and HDL₃-cholesterol (r = 0.253, P < 0.05). Erythocyte catalase activity and TAS were lowered. TAS showed significant negative correlation with Pb_B. A group of workers with Pb_B≥ 400 μg/L had significantly lower Cat_E, lower TAS, and lower HDL₃-cholesterol, compared to the workers with Pb < 400 μg/L. We have also found positive correlation between alpha-tocopherol and total cholesterol (r = 0.267, P < 0.05) and between alpha-tocopherol and LDL-cholesterol (r = 0.207, P < 0.05).     

再障头发、血清和骨髓组织钴、锂元素含量的变化

采用原子吸收光谱技术测定再障４３例患者头发、血清和骨髓组织的钴、锂元素含量，并与急性白血病２０例、缺铁性贫血１０例和正常人４４例作对照比较，再障头发、骨髓钴、锂和血清钴含量均低于正常人，Ｐ＜０．０５，而与急性白血病和缺铁性贫血之间，以及各种类型再障之间则无差异性．再障头发锂含量与血红蛋白、红细胞数呈负相关，Ｐ＜０．０５，说明再障确实存在着钴、锂元素代谢异常．     

肾病综合征与锌铜铁

以临床典型肾病综合征病案为例，地中医中药传统理论和微量元素研究关系，重点了微量元素锌、铜、铁与肾病综合征的病因病机和治疗关系。