用表面增强喇曼光谱研究8种席夫碱化合物及其对脱氧核糖核酸的作用

研究了Ｎ－β－萘酚醛－Ｄ－氨基葡萄糖夫碱及其７种新金属络合物的表面增强喇曼光谱，探讨了它们的振动光谱特征，发现它们在银胶上的吸附方式并不相同，因而在ＳＥＲＳ光谱中存在着明显差异。用ＳＥＲＳ光谱研究了它们与ＤＮＡ的作用。     

D—氨基葡萄糖单配金属配合物与DNA作用的光谱研究比较

研究了５种Ｄ－氨基葡萄糖金属配合物的表面增强喇曼光谱（ＳＥＲＳ），发现它们在银胶上的吸附方式基本相同，因而有相似的ＳＥＲＳ光谱，并用ＳＥＲＳ光谱研究了它们对ＤＮＡ的作用。     

自适应平方根卡尔曼滤波法校正ICP－AES光谱干扰的研究

自适应平方根卡尔曼滤波法校正ＩＣＰ－ＡＥＳ光谱干扰的研究李划新，朱明华，邵济才（华东理工大学分析测试中心上海２００２３７）关键词自适应平方根卡尔曼滤波，ＩＣＰ－ＡＥＳ，光谱干扰光谱干扰是ＩＣＰ－ＡＥＳ分析中不可忽视的问题，特别对光谱复杂物测定时更为严...     

Cu（Ⅱ）NEAE和Cu（Ⅱ）NACN的NIR FT－SERS光谱研究

用沉积银胶的银镜作活性载体，获得了中位－四－（４－Ｎ－乙氧羧甲基吡啶）卟啉络铜（Ⅱ）（Ｃｕ（Ⅱ）ＮＥＡＥ）、中位－四－（４－Ｎ－氰甲基吡啶）卟啉络酮（Ⅱ）（Ｃｕ（Ⅱ）ＮＡＣＮ）的近红外富里叶变换－表面增强喇曼光谱（ＮＩＲＦＴＳＥＲＳ），对其谱峰位移作了归属，并与它们的可见激光表面增强共振喇曼光谱（ＳＥＲＲＳ）作了比较。     

杂原子Me—VPI—5（Me=Mg,Ti,Sn,Si）相转变及其对Eu（Ⅲ）光谱…

用水热同晶置换法合成了杂原子磷酸名分子筛Ｍｅ－ＶＰＩ－５（Ｍｅ＝Ｍｇ，Ｔｉ，Ｓｎ，Ｓｉ）。通过水相离子交换法掺杂稀土离子，考察了高温和高压下的相变行为。讨论了相变过程对稀土离子光谱的影响，随着温度升高Ｅｕ（Ⅲ）Ｍｇ－ＶＰＩ－５先转变为Ｅｕ（Ⅲ）－ＡｌＰＯ４－８，然后又转变成致密的磷石英相。压力对Ｅｕ（Ⅲ）光谱结构具有显著影响。随着压力增加，Ｅｕ（Ⅲ）光谱结构发生规律性变化。     

杂原子Me－VPI－5（Me＝Mg，Ti，Sn，Si）相转变及其对Eu（Ⅲ）光谱结构的研究

用水热同晶置换法合成了杂原子磷酸铝分子筛Ｍｅ－ＶＰＩ－５（Ｍｅ＝Ｍｇ，Ｔｉ，Ｓｎ，Ｓｉ）．通过水相离子交换法掺杂稀土离子，考察了高温和高压下的相变行为．讨论了相变过程对稀土离子光谱的影响．随着温度升高，Ｅｕ（Ⅲ）Ｍｇ－ＶＰＩ－５先转变为Ｅｕ（Ⅲ）－ＡｌＰＯ４－８，然后又转变成致密的磷石英相．压力对Ｅｕ（Ⅲ）光谱结构具有显著影响．随着压力增加，Ｅｕ（Ⅲ）光谱结构发生规律性变化．     

异喹啉及其衍生物的表面增强喇曼光谱研究

采用表面增强喇曼光谱（ＳＥＲＳ）技术获得了异喹啉及其衍生物（３－羧基异喹啉、１－羧基异喹啉、甲基－３－异喹啉羧酸酯、１－羟基异喹啉、５－羟基异喹啉、１，５－二羟基异喹啉）的ＳＥＲＳ谱图，谱带分析表明由于异喹啉环上取代基不同、取代位置不同，均可导致其ＳＥＲＳ光谱的变化。其中１－羟基异喹啉、甲基－３－异喹啉羧酸酯、３－羧基异喹啉、异喹啉是垂直吸附于银胶表面。而１，５－二羟基异喹啉与５－羟基异喹啉则是通过环上π电子云平躺吸附于银胶表面。     

沙棘果皮水溶性多糖Hn的结构研究

沙棘果皮水溶性多糖经酸性乙醇分级和ＤＥＡＥ－ＳｅｐｈａｄｅｘＡ－２５ｓｙｇｇ层析纯化,得到多糖Ｈｎ,经ＳｅｐｈａｒｏｓｅＣＬ－４Ｂ柱层析、玻璃纤维纸电泳、比旋光度测定等方法鉴定Ｈｎ为均一多糖。经唾液淀粉酶解、部分酸水解、高碘酸氧化、Ｓｍｉｔｈ降解、甲基化分析及ＩＲ,＾１３Ｃ－ＮＭＲ,ＧＣ和ＧＣ－ＭＳ等方法,确定其基本结构中主链由１→４ｌｃ基,１→６Ｍａｎ基和１→６Ｇａｌ基构成。三者摩尔比为ｎＧｌｃ     

中位—四（4—N—氰甲基吡啶）卟啉—铜（Ⅱ）络合物及其与脱氧核糖核酸…

本文在银胶活性基质上获得了Ｃｕ（Ⅱ）的中位－四（４－Ｎ－氰甲基吡啶）卟啉络合物Ｃｕ（Ⅱ）ＮＡＣＮ的表面增强喇曼光谱光谱，并与其ＲＲＳ光谱作了比较，讨论了ＰＨ值和Ｃｕ（Ⅱ）ＮＡＣＮ的复合物的ＳＥＲＳ光谱的影响，研究了小牛胸腺双链ＤＮＡ及其变性ＤＮＡ与Ｃｕ（Ⅱ）ＮＡＣＮ的复合物的ＳＥＲＳ光谱，指出Ｃｕ（Ⅱ）ＮＡＣＮ是通过吡啶氮和卟啉核共同与银胶作用的，Ｃｕ（Ⅱ）ＮＡＣＮ是斜向吸附在银表面上的，最佳ＰＨ     

人体基因组课题（HGP）计划给分析化学带来的挑战与机会

本文综述了在ＤＮＡ测序方面分析化学的最新进展，包括电泳，毛细管电泳（ＣＥ），质谱（ＭＳ），电喷雾电离－质谱（ＥＳＩ－ＭＳ），荧光光谱、共振离子谱（ＲＩＳ）和单分子测定，同时还叙述了两维技术和多段测序方法的进展。     

Purification and characterization of anti-complementary polysaccharide from leaves of Thymus vulgaris L.

For the purification of the anti-complementary polysaccharide from Thymus vulgaris L., the hot-water extract of thyme leaves was successively fractionated by ethanol precipitation and ultra-filtration. The retentate with 300-kDa membrane cartridge showed a potent anti-complementary activity. It was further purified by open column chromatographies on DEAE-Toyopearl 650C, Butyl-Toyopearl 650M and Sephadex G-100, obtaining TV3-IIA-I, the purified anti-complementary polysaccharide. The anti-complementary polysaccharide exhibited the anti-complementary activity via both classical and alternative pathways. Based upon the methylation analysis and the reaction with beta-glucosyl Yariv reagent, the anti-complementary polysaccharide from thyme might contain an arabinogalactan moiety, at least in part.     

枸杞功能性成分的提取及其组成的液相色谱分析

对枸杞子中的水溶性多糖成分进行了分离提取及组成分析。采用水提、醇沉等方法提取枸杞子中的多糖成分,用Sevage法除去蛋白质后,用凝胶色谱法进行多糖的纯化,制备3-甲基-1-苯基-2-吡唑啉酮（PMP）衍生物进行液相色谱分析。结果表明,枸杞多糖由鼠李糖、阿拉伯糖、木糖、葡萄糖、半乳糖5种单糖组成,其含量分别为5．28％,17．87％,38．29％,17．82％,20．74％。     

兽疫链球菌变异株产生的透明质酸的纯化及表征

用N-甲基-N′-硝基-N-亚硝基胍(NTG)对兽疫链球菌进行诱变,获得高产菌株.经过对该菌株的发酵培养,将产生的多糖经Savage法、季铵复合物沉淀法、DEAE-纤维素(DE52)离子交换层析法及SephadexG-75凝胶过滤法分离纯化.纯化的多糖结构经化学组成分析、核磁共振波谱、红外光谱及圆二色谱鉴定,证明了诱变得到的高产菌株(StreptococcuszooepidemicusJ18)再经发酵,得到的多糖为透明质酸.通过刚果红实验证明了透明质酸的构象为单股螺旋结构,其平均分子量约为1.16×106.     

白花蛇舌草免疫多糖结构的研究

白花蛇舌草(Hedgotis diffusa willd)系茜草科(Rubiaceae)耳草植物,民间常用此草内服治疗小儿疳疾、毒蛇咬伤、癌肿及肠道疾病,外用治疗各种疮疥及跌打刀伤等症.本文首次报道从白花蛇舌草水溶性成份中分离得到一个具有免疫活性的多糖,并对它的结构进行了初步的探讨.     

Purification, characterization and antioxidant activities in vitro and in vivo of the polysaccharides from Boletus edulis bull

A water-soluble polysaccharide (BEBP) was extracted from Boletus edulis Bull using hot water extraction followed by ethanol precipitation. The polysaccharide BEBP was further purified by chromatography on a DEAE-cellulose column, giving three major polysaccharide fractions termed BEBP-1, BEBP-2 and BEBP-3. In the next experiment, the average molecular weight (Mw), IR and monosaccharide compositional analysis of the three polysaccharide fractions were determined. The evaluation of antioxidant activities both in vitro and in vivo suggested that BEBP-3 had good potential antioxidant activity, and should be explored as a novel potential antioxidant.     

灵芝多糖的结构特征分析

采用沸水回流法从灵芝子实体中提取多糖,经Sevage法除蛋白,乙醇沉淀,离心、透析、膜分离,浓缩、冻干后得灵芝多糖。经HIO4氧化、Smith降解及甲基化反应,并利用多糖及刚果红混合液在碱性溶液中的波长的红移变化,通过UV-VIS,IR,GC,GC-MS,NMR对灵芝水提多糖的结构特征及三螺旋体结构进行分析研究。结果表明:灵芝多糖含有三螺旋体构型,GC-MS分析灵芝多糖的主要单糖组分为葡萄糖,还有少量的半乳糖、甘露糖、木糖和艾杜糖,IR及1HNMR分析多糖为β-构型,HIO4氧化、Smith降解和甲基化分析表明:多糖主要为(1→3)糖苷键连接构型,并伴有少量的1→6位支链键连接的结构,灵芝多糖是由D-葡萄糖单元通过β-(1→3)糖苷键连接葡聚多糖,其主要构型特征为(1→3)β-D-线性连接的骨架结构。     

佛手多糖的化学组成及体外抗氧化活性研究

佛手经热水提取和乙醇沉淀,得佛手多糖粗品(BP).BP经Servag法除蛋白,用DEAE离子交换柱梯度洗脱分离得到BP1和BP2两个组分,经凝胶色谱和SDS-聚丙烯酰胺凝胶电泳检验为均一组分.用凝胶渗透色谱法测得BP1和BP2的重均分子量分别为17773和65589.薄层色谱和高效液相色谱分析表明,BP1和BP2均由鼠李糖、甘露糖、葡萄糖、半乳糖和木糖组成.采用化学发光法在多种化学模拟体系中研究了佛手多糖清除活性氧的作用,观察了佛手多糖对HO.导致DNA链损伤的抑制作用.结果表明,佛手多糖能有效地清除O2-.和HO.等活性氧,对DNA链具有良好的保护作用.     

HPAEC‐PAD method for the analysis of alkaline hydrolyzates of Haemophilus influenzae type b capsular polysaccharide

A gradient method has been devised for the rapid analysis of alkaline hydrolyzates of Haemophilus influenzae type b (Hib) capsular polysaccharide‐based vaccines by high‐performance anion exchange chromatography with pulsed amperometric detection (HPAEC‐PAD). As compared with published procedures, peak shape and sensitivity were significantly improved with this approach, analysis time was short and there was little interference from impurities. The limits of detection and quantification were established with a purified reference polysaccharide. We propose this method as a practical alternative for the analysis of minute amounts of Hib polysaccharide, which can be lower than with the conventional approaches. Copyright © 2013 John Wiley & Sons, Ltd.     

High-sensitivity detection of polysaccharide using phosphodiesters quaternary ammonium salt as probe by decreased resonance light scattering

Phosphodiesters quaternary ammonium salt (PQAS) displayed quite intense light scattering in aqueous solution under the optimum condition. In addition, the resonance light scattering (RLS) signal of PQAS was remarkably decreased after adding trace amount polysaccharide with the maximum peak located at 391 nm. It was found that the decreased RLS intensity of the PQAS − PPGL system (ΔI_RLS) was in proportion to PPGL concentration in the range of 0.1-30 ng mL⁻¹, with a lower detection limit of 0.05 ng mL⁻¹. Based on this rare decreased RLS phenomenon, the novel method of the determination of purified polysaccharide of Gracilaria Lemaneiformis (PPGL) at nanogram level was proposed in this contribution. The proposed approach was used to determine purified polysaccharide extracted from Gracilaria Lemaneiformis with satisfactory results. Compared with the reported polysaccharide assays, this proposed method has good selectivity, high sensitivity and is especially simple and convenient. Moreover, the mechanism of the reaction between PQAS and polysaccharide was investigated by RLS, fluorescence, and fluorescence lifetime spectra.     

A study of the products of the hydrolysis of the xylan ofMelilotus albus by endo-1 4-β-xylanase

The enzymatic hydrolysis of a 4-0-methylglucuronoxylan of white sweetclover (Melilotus albus) by a highly purified homogeneous endo-1, 4-β-xylanase fromAspergillus niger 14 has shown that the enzyme hydrolyzes 97% of the polysaccharide in 72 h. Acidic and neutral oligosaccharides were found in the hydrolysate after the action of the enzyme. An investigation of the hexauronic acid isolated has shown that the glucuronic acid is attached to the nonreducing end of the xylooligosaccharide, which demonstrates the specific action of the enzyme on the polysaccharide.