共查询到20条相似文献,搜索用时 31 毫秒
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继文献[1],我们进一步研究了修饰剂同时也作用于辅助酶的情况下,偶联法测活体系中酶活性不可逆改变动力学。结果表明,在一定条件下,邹承鲁动力学方法仍可应用于偶联法测活体系。 相似文献
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吸附方波伏库仑法研究:I.简单吸附体系 总被引:1,自引:1,他引:0
对简单吸附体系方法伏库仑法进行了理论推导和实验验证,讨论了各种因素对伏库仑曲线的影响,研究表明,对于简单吸附体系,方法伏库仑法的灵敏度和检测限均优于方波伏安法。 相似文献
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对受前行化学反应控制的络合吸附催化体系循环叠式方波伏安法进行了理论推导和实验验证,讨论了电流的特性。结果表明,对于络合吸附催化体系的测定,循环叠式方波伏安法的灵敏度比现行方波伏安法高约25倍。 相似文献
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荧光光度法测定痕量铜的研究 总被引:5,自引:0,他引:5
本文撰写了荧光法测定铜的新方法,详细研究了Cu-硫胺素体系测铜的最佳条件,该法简便、选择性好,灵敏度高,检测限可达0.25ng/ml,用于人发和纯铝样品中铜含量的测定,结果令人满意。 相似文献
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雌激素受体(ER)测定方法及灵敏度问题早已引起临床医学家的关注。七十年代,国外采用饱和活性炭吸附法(DCC)测ER。DCC法简便但不精确,更不能区分受体亚型。蔗糖密度梯度离心法(SDGC)虽能克服DCC法的缺陷,但要求220,000g×16h的离心,受体部分失活降解,不可能作为常规分析用。国外于八十年代开始用HPLC分析ER。本文用大鼠子宫做实验材料,建立了排阻层析HPLC分析ER的方法,并对人子宫内膜及腺癌、乳腺癌组织中的ER做了分析。 相似文献
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本实验利用两测量波长法提高三波长光光度法的灵敏度,选择合适的波长组合,消除两种共存组分的干扰,使△A=Aλ1+Aλ2-KAλ3只与待测组分的浓度有关,灵敏度一般均高于单波长法。以相对误差为选择波长组合的标准,编制了程序,用计算机选择合适的波长组合,验证结果令人满意。 相似文献
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《Analytical letters》2012,45(2):201-211
Abstract A rapid, simple and specific procedure for the simultaneous assay and determination of enantiomeric purity of pharmaceutical grade dextroamphetamine is described. The procedure is faster, more precise and more specific for the dextroisomer than the U.S.P. method. 相似文献
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吡哆辛(VB6)在碱液中有强的光(λex316nm,λem444nm,寿命1.8s),经紫外光照射后光消失.光照前后光强度差(ΔP)与吡哆辛含量呈线性关系.建立了吡哆辛的低温光分析法,并应用于制剂及一些生物组织中吡哆辛含量分析. 相似文献
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The synthesis of a dendrimeric, internally quenched, fluorogenic peptide allowed signal amplification following enzymatic cleavage. 相似文献
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The effects of various classes of organic compounds and of metal ions on the catalytic activity of horseradish peroxidase in hydrogen peroxide-catalysed o-dianisidine oxidation and, on the activity of alkaline phosphatase in p-nitrophenyl phosphate hydrolysis have been studied. Enzymic methods have been developed for determination of sulphur compounds at 10(-5)-10(-4)M, nitrogen compounds at 2 x 10(-7)-3 x 10(-5)M mercury at 3 x 10(-7) mu/ml and lead at 6 x 10(-4) mu/ml concentration. 相似文献
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《Analytical letters》2012,45(14):1443-1449
Abstract Folates in milk are heat labile and methods used to protect these folates during sample preparation for microbiological estimation of this vitamin result in opaque solutions unsuitable for turbidimetric assays. This has necessitated the use of titrimetric assay for milk folates which are long and cumbersome. By the use of rennin precipitation of casein under conditions which preserve the folate activity, an optically clear solution is obtained which can then be used for turbidimetric assay. This method is described in this paper. 相似文献
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《Analytical letters》2012,45(9):757-761
Abstract A quantitative assay for linalool in a commercial sample of coriander oil is described. Good agreement was obtained with results found by the acetylation method. No interference from other oil constituents was noted. 相似文献
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《Analytical letters》2012,45(8):683-689
Abstract A very sensitive analytical technique is described for measuring phosphodiesterase (PDE) activity in mouse muscle, where levels of these enzymes are very low. After the action of PDE, a second enzyme, 5′nucleotidase, is employed to release inorganic phosphorus (Pi) which is assayed by a colorimetric method using malachite-green as dye solution. It is thus possible to measure very small amounts of Pi (0.1 – 0.5 μg) and to assay PDE activity with all the cyclic nucleotides even in the presence of methyl-xanthines. Phosphodiesterase assay in this manner gives a response that is linear with serial amounts of purified enzyme and different quantities of muscle homogenate. 相似文献